Optimization and Standardization of Plant-Derived Vascular Scaffolds DOI Open Access

Gianna Imeidopf,

Dara Khaimov,

Sashane John

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2752 - 2752

Published: March 19, 2025

Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable biocompatible alternative synthetic grafts autologous vessels. We refined decellularization protocols achieve >95% DNA removal while preserving mechanical properties comparable native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based Trypsin/Tergitol-based treatments, achieved via clearing in bleach Triton X-100 for 6 72 h. To assess the environmental influence on scaffold performance, from multiple collection sites processed protocols. Scaffold performance was evaluated through tensile testing histological analysis structural integrity, quantification recellularization measured biological compatibility. Sodium sulfate-treated with shorter durations demonstrated highest (≥95%) properties, outperforming Trypsin/Tergitol treatments. Longer times reduced fiber diameter by 60%, compromising strength. Shorter preserved integrity improved seeding efficiency. Larger supported higher densities than smaller leaves, highlighting need standardized material sources. Permeability tests minimal leakage at 120 mmHg stability under dynamic flow conditions, suggesting their suitability vascular applications. These findings establish reliable framework optimizing grafts, improving reproducibility tissue engineering

Language: Английский

Optimization and Standardization of Plant-Derived Vascular Scaffolds DOI Open Access

Gianna Imeidopf,

Dara Khaimov,

Sashane John

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2752 - 2752

Published: March 19, 2025

Vascular graft failure rates remain unacceptably high due to thrombosis and poor integration, necessitating innovative solutions. This study optimized plant-derived extracellular matrix scaffolds as a scalable biocompatible alternative synthetic grafts autologous vessels. We refined decellularization protocols achieve >95% DNA removal while preserving mechanical properties comparable native vessels, significantly enhancing endothelial cell seeding. Leatherleaf viburnum leaves were decellularized using sodium dodecyl sulfate-based Trypsin/Tergitol-based treatments, achieved via clearing in bleach Triton X-100 for 6 72 h. To assess the environmental influence on scaffold performance, from multiple collection sites processed protocols. Scaffold performance was evaluated through tensile testing histological analysis structural integrity, quantification recellularization measured biological compatibility. Sodium sulfate-treated with shorter durations demonstrated highest (≥95%) properties, outperforming Trypsin/Tergitol treatments. Longer times reduced fiber diameter by 60%, compromising strength. Shorter preserved integrity improved seeding efficiency. Larger supported higher densities than smaller leaves, highlighting need standardized material sources. Permeability tests minimal leakage at 120 mmHg stability under dynamic flow conditions, suggesting their suitability vascular applications. These findings establish reliable framework optimizing grafts, improving reproducibility tissue engineering

Language: Английский

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