Efficient production system for hydrogel-based transparent soil for plant root observation DOI Creative Commons
Naoyuki Sotta, P. R. Li, Toru Fujiwara

et al.

BioTechniques, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 5

Published: Jan. 31, 2025

Observation of plant root morphology in soil is fundamental importance research, but the lack transparency hampers direct observation roots. One approaches to overcome this technical limitation use "transparent soil" (TS), hydrogel-based beads produced by spherification gelling agents. However, production TS natural dripping solution can be labor intensive, time consuming and difficult maintain consistent product quality. Here we present a semi-automated system for production. A three-channel peristatic pump controls critical parameters spherification, such as drop height ionic strength, allowing larger-scale with less manual operation. This improves efficiency experiments using enables large-scale requiring large amounts TS.

Language: Английский

Efficient production system for hydrogel-based transparent soil for plant root observation DOI Creative Commons
Naoyuki Sotta, P. R. Li, Toru Fujiwara

et al.

BioTechniques, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 5

Published: Jan. 31, 2025

Observation of plant root morphology in soil is fundamental importance research, but the lack transparency hampers direct observation roots. One approaches to overcome this technical limitation use "transparent soil" (TS), hydrogel-based beads produced by spherification gelling agents. However, production TS natural dripping solution can be labor intensive, time consuming and difficult maintain consistent product quality. Here we present a semi-automated system for production. A three-channel peristatic pump controls critical parameters spherification, such as drop height ionic strength, allowing larger-scale with less manual operation. This improves efficiency experiments using enables large-scale requiring large amounts TS.

Language: Английский

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