Ecogenomic insights into the resilience of keystone Blastococcus Species in extreme environments: a comprehensive analysis

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 20, 2025

Language: Английский

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Insights into the genome of Azotobacter sp. strain CWF10, isolated from an agricultural field in Central India

Access Microbiology, Journal Year: 2025, Volume and Issue: 7(1)

Published: Jan. 1, 2025

Azotobacter sp. strain CWF10, an aerobic gram-negative, oval-shaped and motile bacterium, was isolated from the lateritic agricultural soil of Madhya Pradesh, India. The draft genome isolate is 5.7 Mb in size, consisting 14 contigs with 65.09% G+C content. Average nucleotide identity (94.66%) digital DNA–DNA hybridization (62%) calculation closest reference strains underpin bacterium as a potential novel species. has plethora plant growth-promoting genes that point towards ability to enhance available nitrogen biosynthesis folic acid, among others. Siderophores such vibrioferrin crochelin A are also present known regulate iron uptake. Overall, mining CWF10 revealed this for application regenerative agriculture sustaining health.

Language: Английский

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Pangenome analysis of Bacillus velezensis exploring the probiotic potential and plant growth promotion traits of strains isolated from fish intestines

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: Feb. 18, 2025

Language: Английский

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Genomic Blueprint of Four Soil Bacteria with Insights into their Potential Adaptation Mechanisms in Tropical Savanna

Ecological Genetics and Genomics, Journal Year: 2025, Volume and Issue: unknown, P. 100351 - 100351

Published: April 1, 2025

Language: Английский

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Plant pathogens, microbiomes, and soil health

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Pseudomonas machongensis sp. nov. and Stenotrophomonas capsici sp. nov., isolated from wilted pepper plants

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(11)

Published: Nov. 26, 2024

Two Gram-stain-negative, aerobic, rod-shaped, non-endospore-forming bacteria, designated as strain MH1 T and MH2 , were isolated from branches of wilted pepper plants ( Capsicum annuum ) collected a farmland in Machong town, Guangdong, China, investigated using polyphasic approach. grew at temperatures 4–42 °C (optimum 28 °C), with 0–6.0 % (w/v) NaCl pH 4.0–10.0 4.0). 0–6.0% 5.0). Analysis the 16S rRNA gene sequence indicated that belongs to Stenotrophomonas Pseudomonas . Genome-based phylogenetic analysis further established shares closest evolutionary relationships humi DSM 18929 terrae 18941, is sister wayambapalatensis RW3S1. Whole-genome comparisons between known species revealed average nucleotide identity (ANI) values up 84.5%, well digital DNA–DNA hybridization (dDDH) 28.3%, both substantially lower than accepted thresholds for delineation (ANI: 95%; dDDH: 70%). The ANI dDDH most 94.6 59.2 %, respectively. Additional biochemical physiological analyses support represent novel Notably, differences carbon source utilization could differentiate its close relatives major fatty acids iso-C 15 : 0 16 14 C 18 1 ω7c/C ω6c (summed feature 8), 3) 17 cyclo Therefore, we propose new capsici sp. nov., (=GDMCC 1.3749 =JCM 36317 type strain, machongensis 1.3750 36318 strain. genome has size 4.18 Mb GC-content 67.19 mol%, while 5.71 63.12 mol%.

Language: Английский

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Genomic Blueprint of Novel Slow-Growing Bacteria with Insights into their Potential Adaptation Mechanisms in Soil Ecosystems

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 11, 2024

Slow-growing bacteria play a crucial role in soil ecosystems, yet our understanding of their persistence complex environments remains limited. Do these organisms lack essential metabolic genes, or do they possess specific genes that enable survival under challenging conditions? Our study begins to fill this knowledge gap by analyzing the genomes four slow-growing isolated from tropical savanna soil, providing insights into capacities and adaptation mechanisms. findings revealed represent novel species, including new strains Bosea, Nocardioides, Cupriavidus, Enterobacter roggenkampii. Most encoded key pathways related central metabolism, particularly sugar amino acid suggesting slow growth may be an adaptive strategy. The also exhibited potential contributions vital biogeochemical processes, carbon, nitrogen, sulfur cycling, as well plant promotion. We identified 164 defense across 32 families at least one antimicrobial resistance (AMR) gene each strain, with strain CER55 showing highest number predicted AMR genes. Additionally, twelve biosynthetic clusters were three strains. combination mechanisms, resistance, secondary metabolite production indicates evolved multiple strategies thrive competitive microbial communities.

Language: Английский

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Genome Insights into Beneficial Microbial Strains Composing SIMBA Microbial Consortia Applied as Biofertilizers for Maize, Wheat and Tomato

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2562 - 2562

Published: Dec. 12, 2024

For the safe use of microbiome-based solutions in agriculture, genome sequencing strains composing inoculum is mandatory to avoid spread virulence and multidrug resistance genes carried by them through horizontal gene transfer other bacteria environment. Moreover, annotated genomes can enable design specific primers trace into soil provide insights molecular genetic mechanisms plant growth promotion biocontrol activity. In present work, sequences some members beneficial microbial consortia that have previously been tested greenhouse field trials as promising biofertilizers for maize, tomato wheat crops determined. Strains belong well-known plant-growth-promoting bacterial genera such Bacillus, Burkholderia, Pseudomonas Rahnella. The size ranged from 4.5 7.5 Mbp, carrying many spanning 4402 6697, a GC content 0.04% 3.3%. annotation revealed presence are implicated functions related antagonism, pathogenesis secondary metabolites possibly involved clusters protection against oxidative damage, confirming (PGP) activity selected strains. All target were found possess at least 3000 different PGP traits, belonging categories nitrogen acquisition, colonization plant-derived substrate usage, quorum sensing response biofilm formation and, lesser extent, fitness root colonization. No putatively identified. Overall, our study suggests application “plant probiotics” sustainable agriculture.

Language: Английский

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Is Pseudofrankia, the non-nitrogen-fixing and/or non-nodulating actinorhizal nodule dweller, mutualistic or parasitic? Insights from genome-predictive analysis

International Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

Language: Английский

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