Sustainability,
Journal Year:
2024,
Volume and Issue:
16(7), P. 3045 - 3045
Published: April 6, 2024
This
study
aimed
to
enhance
the
nutrient
balance
of
municipal
solid
waste
(MSW),
characterized
by
a
high
carbon-to-nitrogen
(C/N)
ratio,
which
is
critical
factor
in
anaerobic
digestion
process.
The
investigation
involved
addition
MSW,
rich
carbon
content,
food
(FW)
with
nitrogen
content.
goal
was
determine
an
optimal
co-substrate
mixing
ratio
MSW
and
FW
for
co-digestion
at
mesophilic
temperatures,
aiming
improve
process
stability
performance
achieve
higher
biogas
yield.
experiments
encompassed
five
ratios
C/N
20,
25,
30,
35,
40
under
conditions
laboratory.
results
indicated
that
highest
specific
yield,
reaching
827
L/kg
VS,
attained
when
feedstock
had
balanced
surpassing
520
vs.
obtained
from
alone.
As
proportion
increased
feedstock,
production
rate
decreased.
Additionally,
explored
substrate-to-inoculum
(S/I)
focusing
on
20.
Four
S/I
(0.5,
1.0,
1.5,
2.0)
were
examined,
revealing
642
occurred
0.5.
An
accumulation
volatile
fatty
acids
(VFAs)
observed
ratios,
attributed
lower
abundance
inoculum
microorganisms
Overall,
findings
suggested
optimum
falls
within
range
20–25/1,
while
preferred
Journal of Physics Conference Series,
Journal Year:
2025,
Volume and Issue:
2969(1), P. 012006 - 012006
Published: Feb. 1, 2025
Abstract
The
start-up
of
large
biogas
projects
required
a
number
inoculum,
which
need
high
cost
and
long
time.
This
study
investigated
three
methods
on
starting
the
Continuous
Stirred
Tank
Reactor
(CSTR)
corn
stalk.
used
domesticated
cow
dung
as
inoculum
feeding
frequency
included
every
2
d
(T2),
4
(T4),
8
(T8).
results
shown
that
CSTR
reactor
stalk
could
be
started
successfully
under
different
methods.
Among
them,
T4
achieved
highest
cumulative
methane
production
with
66.93L,
was
11.86%
36.15%
higher
than
T2
T8.
Compared
manure
system,
microbial
diversity
in
reactors
increased.
Firmicutes
dominant
bacterial
phyla,
Methanosarcina
archaea.
enrichment
corresponded
to
production.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 31, 2025
Abstract
High
total
ammonia
nitrogen
(TAN)
inhibits
anaerobic
digestion
(AD)
and
cannot
be
completely
eliminated
by
merely
enhancing
a
stage
of
AD.
This
study
incorporates
TAN‐tolerant
inoculum
into
substrates
hydrolyzed
Rhizopus
mixed
agents
to
simultaneously
enhance
hydrolysis‐acidogenesis‐methanogenesis.
The
results
show
16.46‐fold
increase
in
CH4
production
under
TAN‐inhibited
(6870.97
mg
L−1)
conditions,
even
exceeding
the
AD
without
TAN
21.10%.
Model
sodium
acetate
H2
confirm
hydrogenotrophic
methanogenesis
is
main
pathway,
with
reduced
inhibition.
Furthermore,
synergistic
metabolic
microbial
community
dominated
hydrolytic
bacteria
JAAYGG01
sp.
DTU014
sp.,
acidogenic
DTU015
DTU013
JAAYLO01
methanogens
Methanosarcina
mazei
an
unclassified
species
Methanoculleus
reconstructed
resist
Metagenomic
combined
metatranscriptomic
sequencing
identifies
that
this
carries
xynD
bglB
regulate
substrate
hydrolysis,
leading
through
glycolysis,
butyrate,
pyruvate
metabolism
high
kinase
activity,
thereby
produced
primarily
via
coenzyme
F420
facilitated
efficient
mass
transfer
processes
quorum
sensing
regulation.
cleaner
strategy
obtains
higher
economic
benefit
(US$149.02)
than
conventional
can
154.64‐fold
energy
24
000
m3
biogas
plant,
guided
machine
learning.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(8), P. 1766 - 1766
Published: April 15, 2025
Anaerobic
digestion
(AD)
is
a
preferred
method
for
food
waste
(FW)
treatment
due
to
its
sustainability
and
potential
production
of
renewable
bioenergy.
However,
the
accumulation
volatile
fatty
acids
(VFAs)
ammonia
often
destabilizes
AD
process,
managing
digestate
byproduct
poses
additional
challenges.
This
study
investigates
use
co-pyrolysis
biochar
synthesized
from
rice
straw
(DRB)
enhance
methane
efficiency.
DRB
addition
increased
cumulative
yield
by
37.1%,
improved
VFA
conversion
efficiency,
achieved
42.3%
higher
NH3-N-removal
rate
compared
control
group.
The
COD-removal
was
68.7%
throughout
process.
Microbial
analysis
revealed
that
selectively
enriched
Fastidiosipila
Methanosarcina,
promoting
direct
interspecies
electron
transfer
(DIET)
yield.
These
findings
highlight
DRB’s
efficiency
support
closed-loop
resource
utilization.
