A twin of Polydora hoplura (Annelida: Spionidae) from the Arabian (Persian) Gulf, with review of primers used for barcoding of Spionidae DOI
Vasily I. Radashevsky, Manal Al‐Kandari,

Vasily V. Malyar

et al.

Zootaxa, Journal Year: 2024, Volume and Issue: 5529(2), P. 245 - 268

Published: Oct. 24, 2024

The spionid polychaete Polydora hoplura Claparède, 1868 has been widely recorded boring in shells of abalone, oysters, clams, barnacle tests and sponges temperate subtropical waters. Molecular studies have suggested conspecificity individuals collected worldwide but showed high genetic variability the species with highest diversity haplotypes South African population. We compared morphology data shell-boring worms from Kuwait, which were previously assigned to P. hoplura, American, Asian European individuals, including those type locality Italy. Kuwaiti share key diagnostic morphological characters differ ochre pigment on anterior chaetigers life, pattern pigmentation after fixation formalin, methyl green staining fixed specimens. They also dimensions mature spermatozoa. analysis sequence five gene fragments (total 3483 bp) that intraspecific Kuwait are less than divergences all studied genes, except for 28S rDNA, between these two groups. These data, as well absence common cytochrome c oxidase subunit I (COI) 16S haplotypes, differences allowed us conclude population is not conspecific hoplura. This conclusion was confirmed by results delimitation analysis. In Bayesian inference formed a well-supported clade sister described illustrated here new species, mohammadi sp. nov. Primers used successful amplification mitochondrial COI various Spionidae reviewed we suggest future use combination primer pairs (2F–spionid–LCO/1R–spionid–HCO Dorid_COI.3F/Dorid_COI.1R) target sequences include barcode covered “Folmer” “Dorid” primers.

Language: Английский

A twin of Polydora hoplura (Annelida: Spionidae) from the Arabian (Persian) Gulf, with review of primers used for barcoding of Spionidae DOI
Vasily I. Radashevsky, Manal Al‐Kandari,

Vasily V. Malyar

et al.

Zootaxa, Journal Year: 2024, Volume and Issue: 5529(2), P. 245 - 268

Published: Oct. 24, 2024

The spionid polychaete Polydora hoplura Claparède, 1868 has been widely recorded boring in shells of abalone, oysters, clams, barnacle tests and sponges temperate subtropical waters. Molecular studies have suggested conspecificity individuals collected worldwide but showed high genetic variability the species with highest diversity haplotypes South African population. We compared morphology data shell-boring worms from Kuwait, which were previously assigned to P. hoplura, American, Asian European individuals, including those type locality Italy. Kuwaiti share key diagnostic morphological characters differ ochre pigment on anterior chaetigers life, pattern pigmentation after fixation formalin, methyl green staining fixed specimens. They also dimensions mature spermatozoa. analysis sequence five gene fragments (total 3483 bp) that intraspecific Kuwait are less than divergences all studied genes, except for 28S rDNA, between these two groups. These data, as well absence common cytochrome c oxidase subunit I (COI) 16S haplotypes, differences allowed us conclude population is not conspecific hoplura. This conclusion was confirmed by results delimitation analysis. In Bayesian inference formed a well-supported clade sister described illustrated here new species, mohammadi sp. nov. Primers used successful amplification mitochondrial COI various Spionidae reviewed we suggest future use combination primer pairs (2F–spionid–LCO/1R–spionid–HCO Dorid_COI.3F/Dorid_COI.1R) target sequences include barcode covered “Folmer” “Dorid” primers.

Language: Английский

Citations

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