Follicular fluid supports cumulus expansion in post-mortem recovered cumulus-oocyte complexes of Hippopotamus amphibius

Theriogenology Wild, Journal Year: 2025, Volume and Issue: unknown, P. 100125 - 100125

Published: March 1, 2025

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А new technology for maturation of human oo cytes by injection of extracellular vesicles of follicular donor fluid under the shiny shell of the female gamete

Translational Medicine, Journal Year: 2025, Volume and Issue: 12(1), P. 17 - 26

Published: May 7, 2025

Background . In assisted reproductive technology (ART) programs, not all oocytes obtained during transvag inal puncture (TVP) are suitable for fertilization, as of them at MII stage. Oocytes germinal vesicle (GV) and MI stages discarded because, after cumulus cells (CC) removal, their maturation fertilization become impossible. Study proposes method maturing by introducing extracellular vesicles (EVs) from follicular fluid (FF) into the perivitelline space. Objective To evaluate clinical feasibility GV/MI oo cytes without injecting donor FF EVs under zona pellucida. Materials methods (5 ml) was collected 4 donors. were isolated using sequential centrifugation. Portion analyzed Nanoparticle Tracking Analysis (NTA), while another portion examined transmission electron microscopy (TEM). Total 53 immature selected main group 18 control group. Donor injected hours CC removal suspension After 17 hours, oocyte maturity assessed in both groups. Results rate statistically significantly higher than group, indicating potential effectiveness EV-IVM GV-stage oocytes. Conclusion Obtained data provide hope development a new vitro maturation.

Language: Английский

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Extracellular vesicles affecting embryo development in vitro: a potential culture medium supplement

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 18, 2024

Extracellular vesicles (EVs) are nanometer-sized lipid bilayer released by cells, playing a crucial role in mediating cellular communication. This review evaluates the effect of EVs on early embryonic development vitro systematically searching literature across three databases, Embase, PubMed, and Scopus, from inception (Embase, 1947; 1996; 2004) to 30 June 2024. A total 28 studies were considered relevant included this review. The these investigations have been recovered range sources, including oviduct fluid, follicular uterine seminal plasma, embryos, epithelial endometrial amniotic endometrial-derived mesenchymal stem cells collected primarily mice, rabbits, cattle pigs. diversity EV sources highlights broad interest potential applications embryo culture systems. These demonstrated that supplementation with derived physiologically normal biofluids medium system has positive effects development. Conversely, under pathological conditions shown negative impact. finding underscores importance source condition used media. Further, addition as supplement holds significant therapeutic for optimizing In conclusion, evaluation offers thorough assessment available data media challenges using production.

Language: Английский

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Preovulatory follicular fluid secretome added to in vitro maturation medium influences the metabolism of equine cumulus-oocyte complexes

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: June 25, 2024

Abstract Background In vitro embryo production is a highly demanded reproductive technology in horses, which requires the recovery (in vivo or post-mortem ) and maturation (IVM) of oocytes. Oocytes subjected to IVM exhibit poor developmental competence compared their counterparts, being this related suboptimal composition commercial media. The objective work was study effect different concentrations secretome obtained from equine preovulatory follicular fluid (FF) on cumulus-oocyte complexes (COCs) during IVM. COCs retrieved by ovum pick up (OPU) slaughterhouse (SLA) were presence absence (Control: 0 µg/ml, S20: 20 µg/ml S40: 40 µg/ml). After IVM, metabolome medium used for oocyte prior (Pre-IVM) after (Post-IVM), mRNA expression, meiotic analysed. Results leads lactic acid an acetic consumption OPU SLA. However, glucose higher when S40 added (Control Pre-IVM vs. Post-IVM: 117.24 ± 7.72 82.69 4.24; Mean µM SEM; p < 0.05), while not observed Likewise, enhanced uptake threonine S20 Post-IVM 4.93 0.33 3.04 0.25 2.84 0.27; 0.05) recovered OPU. Regarding relative expression candidate genes metabolism, Lactate dehydrogenase A (LDHA) significantly downregulated at 20–40 OPU-derived 1.77 0.14 1 1.23 0.14; fold change but SLA COCs. Conclusions addition affects gene LDHA, amino turnover (COCs), with diverging outcomes between using slaughterhouse-derived (SLA).

Language: Английский

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Preliminary construction of non-coding RNAs and ceRNA regulatory networks mediated by exosomes in porcine follicular fluid

Genomics, Journal Year: 2024, Volume and Issue: 116(5), P. 110920 - 110920

Published: Aug. 14, 2024

Follicles are fundamental units of the ovary, regulated intricately during development. Exosomes and ovarian granulosa cells (OGCs) play pivotal roles in follicular development, yet regulatory mechanisms governing exosomes remain elusive.

Language: Английский

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