The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response DOI Creative Commons
Lumin Yu, Yuzhong Zhao, Shanpeng Zhang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that responsible for high mortality, morbidity and severe economic losses to the industry globally, posing substantial risk health of poultry. APEC encounters reactive oxygen species (ROS) during infection process thus has evolved antioxidant defense mechanisms protect against oxidative damage. The imbalance ROS production defenses known as stress, which results in damage proteins, lipids DNA, even bacterial cell death. uses transcription factors (TFs) handle stress. While many TFs E. have been well characterized, mechanism YbdO TF on protecting regulating virulence pathogenicity not clarified. Here we focus regulatory APEC. from this study showed attenuated chicks models by inhibiting expression genes fepG ycgV using quantitative real-time reverse PCR (RT-qPCR) experiments. electrophoretic mobility shift assays (EMSA) confirmed specifically bound promoters ycgV. Additionally, increases H2O2-induced via repressing stress response sodA, soxR, ahpC, ahpF, katG, oxyR binding their promoter regions. repression effect facilitates host immune eliminate generate beneficial protection body, thereby indirectly attenuating These findings might provide further insights into offer new perspectives studies prevention control infections.

Language: Английский

Antimicrobial resistance and virulence factors analysis of a multidrug-resistant Acinetobacter baumannii isolated from chickens using whole-genome sequencing DOI Creative Commons
Lumin Yu, Yuzhong Zhao, Shanpeng Zhang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 19, 2024

Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is currently recognized not only as a significant nosocomial pathogen but also an emerging bacterial infection in food-producing animals, posing critical threat to global health. However, this hindrance detailed bioinformatic studies of MDR A. chicken origin due the lack its complete genome sequence. Here, we report whole-genome sequencing analysis Y03 isolated from chickens. The consists 1 circular chromosome and 4 plasmids, harbors 41 antimicrobial resistance genes conferring major classes antibiotics, including β-lactams, phenicols, macrolides, lincosamides, aminoglycosides, nitrofurans, well 135 virulence factors involved effector delivery system, immune modulation, adherence, stress survival, biofilm, exotoxin, nutritional/metabolic factor. vivo experiments certificated that was virulent Meanwhile, used PCR amplification method detect 10 abeM, adeB, adeH, adeK, blaapmC, blaOXA−90, catB9, macB, folP, parE, 14 lpxC, pilO, fimT, ompA, basA, bauA, gspL, csu, pgaC, plc2, tssA, tviB, bap, vgrG. Whole-genome revealed contained 46 horizontal gene transfer elements, 11 genomic islands, 30 transposons, 5 prophages, 518 mutations associated with reduced 44 resulting loss pathogenicity. Furthermore, there were 22 antibiotic targets 28 lethal on could be potential prevent, control, treat infections caused by Y03. Therefore, study contributes development strategies for prevention, treatment their spread

Language: Английский

Citations

0
The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response DOI Creative Commons
Lumin Yu, Yuzhong Zhao, Shanpeng Zhang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that responsible for high mortality, morbidity and severe economic losses to the industry globally, posing substantial risk health of poultry. APEC encounters reactive oxygen species (ROS) during infection process thus has evolved antioxidant defense mechanisms protect against oxidative damage. The imbalance ROS production defenses known as stress, which results in damage proteins, lipids DNA, even bacterial cell death. uses transcription factors (TFs) handle stress. While many TFs E. have been well characterized, mechanism YbdO TF on protecting regulating virulence pathogenicity not clarified. Here we focus regulatory APEC. from this study showed attenuated chicks models by inhibiting expression genes fepG ycgV using quantitative real-time reverse PCR (RT-qPCR) experiments. electrophoretic mobility shift assays (EMSA) confirmed specifically bound promoters ycgV. Additionally, increases H2O2-induced via repressing stress response sodA, soxR, ahpC, ahpF, katG, oxyR binding their promoter regions. repression effect facilitates host immune eliminate generate beneficial protection body, thereby indirectly attenuating These findings might provide further insights into offer new perspectives studies prevention control infections.

Language: Английский

Citations

0