Anticancer therapeutic potential of benzofuran scaffolds

RSC Advances, Journal Year: 2023, Volume and Issue: 13(16), P. 11096 - 11120

Published: Jan. 1, 2023

Benzofuran moiety is the main component of many biologically active natural and synthetic heterocycles.

Language: Английский

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Emergence of microbial resistance against nanoparticles: Mechanisms and strategies

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 26, 2023

Antimicrobial nanoparticles have gained the status of a new generation drugs that can kill bacterial pathogens by multiple means; however, nanoparticle resistance acquired some has evoked cause concern. Several reports suggested bacteria develop nanoparticles, specifically metal resistance, mechanisms: transformation-induced oxidative stress, membrane alterations, reversible adaptive irreversible modifications to cell division, and change in motility resistance. Surface properties, concentration aggregation biofilm forming exclusion capacity, R plasmid flagellin synthesis are crucial factors development bacteria. Studies reported reversal modifying surface corona or inhibiting production pathogens. Furthermore, strict regulation regarding use disposal nano-waste across globe, firm knowledge microbe–nanoparticle interaction, regulated soil water is required prevent microbes from developing

Language: Английский

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Effect and mechanism of paclitaxel loaded on magnetic Fe ₃ O ₄ @mSiO ₂ -NH ₂ -FA nanocomposites to MCF-7 cells

Drug Delivery, Journal Year: 2022, Volume and Issue: 30(1), P. 64 - 82

Published: Dec. 6, 2022

Magnetic Fe3O4 nanoparticles were prepared via a simple hydrothermal method and utilized to load paclitaxel. The average particle size of was found be 20.2 ± 3.0 nm, the calculated saturation magnetization reached 129.38 emu/g, verifying superparamagnetism nanomaterials. specific surface area pore volume 84.756 m2/g 0.265 cm3/g, respectively. Subsequently, Fe3O4@mSiO2 successfully fabricated using as precursors with an 27.81 nm. relevant magnetization, zeta potential, Fe3O4@mSiO2-NH2-FA respectively 76.3 -14.1 mV, 324.410 m2/g. adsorption 0.369 cm3/g 4.548 Compared free paclitaxel, solubility stability loaded paclitaxel improved. drug loading efficiency nanoformulation 44.26 11.38%, nanocomposites easy construct excellent active targeting performance, pH sensitivity, sustained-release effect. also showed good biocompatibility, where cell viability remained at 73.8% when concentration 1200 μg/mL. induced death through apoptosis, confirmed by AO/EB staining flow cytometry. Western blotting results suggested that could induce iron inhibiting Glutathione Peroxidase 4 (GPX4) activity or decreasing Ferritin Heavy Chain 1 (FTH1) expression. expression HIF-1α upregulated owing accumulation reactive oxygen species (ROS), thus affecting apoptosis-related proteins regulated p53, inducing apoptosis.

Language: Английский

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Optimization of fermentation parameters to improve the biosynthesis of selenium nanoparticles by Bacillus licheniformis F1 and its comprehensive application

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 20, 2024

Abstract Background Selenium nanoparticles (SeNPs) are increasingly gaining attention due to its characteristics of low toxicity, high activity, and stability. Additionally, Bacillus licheniformis , as a probiotic, has achieved remarkable research outcomes in diverse fields such medicine, feed processing, pesticides, attracting widespread attention. Consequently, evaluating the activity probiotics SeNPs is paramount. The utilization synthesize SeNPs, achieving large-scale industrialization, current hotspot field synthesis currently most promising synthetic method. To minimize production costs maximize yield this study selected agricultural by-products that nutrient-rich, cost-effective, readily available culture medium components. This approach not only fulfills industrial requirements but also mitigates impact on downstream processes. Results experimental findings revealed synthesized by B. F1 exhibited spherical morphology with diameters ranging from 110 170 nm demonstrating Both secondary metabolites possessed significant free radical scavenging ability. provide more robust foundation for acquiring large quantities via fermentation F1, key factors were identified through single-factor experiments response surface methodology (RSM) include 2% seed liquid inoculum, temperature 37 ℃, agitation at 180 rpm. critical during optimization process corn powder (11.18 g/L), soybean meal (10.34 NaCl (10.68 g/L). Upon validating optimized conditions medium, can nearly 100.00% 5 mmol/L sodium selenite. Subsequently, pilot-scale verification L fermentor using resulted shortened time, significantly reducing costs. Conclusion In study, efficient probiotic was successfully achieved, leading reduction exploration practical applications strain holds potential provides valuable guidance facilitating industrial-scale implementation microbial SeNPs.

Language: Английский

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Synthesis and activation of pH-sensitive metal–organic framework Sr(BDC)_∞ for oral drug delivery

Dalton Transactions, Journal Year: 2023, Volume and Issue: 53(3), P. 1048 - 1057

Published: Dec. 1, 2023

Metal-organic frameworks (MOFs) are widely used in the biomedical industry. In this study, we developed a new method for obtaining metal-organic structure of strontium and terephthalic acid, Sr(BDC), an alternative activation removing DMF from pores. Sr(BDC) MOFs were successfully prepared characterized by XRD, FTIR, TGA, SEM. The importance steps was confirmed which showed that Sr(BDC)(DMF) sample can contain up to quarter solvent (DMF) before activation. our IR spectroscopy possibility ethanol treatment Sr-BDC crystals. A comparative analysis effect on specific surface pore size its sorption properties using model drug doxorubicin due undeveloped Sr-(BDC)(DMF) sample, it is not possible obtain adsorption isotherm determine distribution, thus showing step. Cytotoxicity apoptosis assays carried out study biological activity MOFs, observed relatively low toxicity tested concentration range after 48 h, with over 92% cell survival Sr(BDC)(260 °C), decrease only highest (800 mg L

Language: Английский

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Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 519 - 519

Published: Jan. 23, 2023

The Western Ghats, India, is a hotspot for lichen diversity. However, the pharmacological importance of lichen-associated metabolites remains untapped. This study aimed to evaluate cytotoxic potential lichens this region. For this, sixteen macrolichens were collected and identified from two locations in Ghats. acetone extract Usnea cornuta (UC2A) showed significant cytotoxicity towards multiple human cancer cell lines. Interestingly, co-treatment with chloroquine (CQ), an autophagy inhibitor, increased UC2A extract. A gas chromatography mass spectrometry (GCMS) revealed usnic acid (UA), atraric barbatic as dominant compounds Further, UA was purified evaluated HeLa cells. monodansyl cadaverine mitotracker red double staining autophagy-inducing activities UA, inhibition confirmed via CQ treatment. Autophagy by 12–16% concentration-dependent manner. It also lipid peroxidation, ROS levels mitochondrial depolarization decreased glutathione availability. decrease zeta 40% increase caspase 3/7 activity noted after treatment UA-treated Thus, can be inhibiting autophagy.

Language: Английский

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FTIR microspectroscopic study of gastric cancer AGS cells apoptosis induced by As2O3

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 311, P. 123998 - 123998

Published: Feb. 8, 2024

Language: Английский

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Multifaceted Properties of Usnic Acid in Disrupting Cancer Hallmarks

Biomedicines, Journal Year: 2024, Volume and Issue: 12(10), P. 2199 - 2199

Published: Sept. 26, 2024

Cancer, a complex group of diseases marked by uncontrolled cell growth and invasive behavior, is characterized distinct hallmarks acquired during tumor development. These hallmarks, first proposed Douglas Hanahan Robert Weinberg in 2000, provide framework for understanding cancer's complexity. Targeting them key strategy cancer therapy. It includes inhibiting abnormal signaling, reactivating suppressors, preventing invasion metastasis, angiogenesis, limiting replicative immortality, modulating the immune system, inducing apoptosis, addressing genome instability regulating cellular energetics. Usnic acid (UA) natural compound found lichens that has been explored as cytotoxic agent against cells different origins. Although exact mechanisms remain incompletely understood, UA presents promising therapeutic intervention. Understanding its impact on provides valuable insights into potential developing targeted multifaceted therapies. This article explores activity context disrupting origins based recent articles emphasize molecular this activity.

Language: Английский

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ATR-FTIR spectroscopy to evaluate serum protein expression in a murine cerebral ischemia model

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2024, Volume and Issue: 326, P. 125261 - 125261

Published: Oct. 9, 2024

Language: Английский

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Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded with Usnea barbata (L.) F. H. Wigg Ethanol Extract F-UBE-HPMC

Antioxidants, Journal Year: 2022, Volume and Issue: 11(9), P. 1801 - 1801

Published: Sept. 13, 2022

The oral cavity’s common pathologies are tooth decay, periodontal disease, and cancer; squamous cell carcinoma (OSCC) is the most frequent malignancy, with a high mortality rate. Our study aims to formulate, develop, characterize, pharmacologically investigate mucoadhesive patches (F-UBE-HPMC) loaded Usnea barbata (L.) F.H. Wigg dry ethanol extract (UBE), using HPMC K100 as film-forming polymer. Each patch contains 312 µg UBE, total phenolic content (TPC) of 178.849 33.924 usnic acid. Scanning electron microscopy (SEM) atomic force (AFM) were performed for their morphological characterization, followed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA). Pharmacotechnical evaluation involved measurement specific parameters follows: weight uniformity, thickness, folding endurance, tensile strength, elongation, moisture content, pH, disintegration time, swelling rate, ex vivo mucoadhesion time. Thus, each F-UBE-HPMC has 104 ± 4.31 mg, pH = 7.05 0.04, time 130 4.14 s, ratio 272 6.31% after 6 h, 102 3.22 min. Then, F-UBE-HPMCs pharmacological effects investigated brine shrimp lethality assay (BSL assay) cytotoxicity prescreening test, complex flow cytometry analyses on blood cultures epithelial CLS-354 line. results revealed significant anticancer considerably increasing oxidative stress blocking DNA synthesis in cancer cells. antimicrobial potential against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa 27353, Candida albicans 10231, parapsilosis 22019 was assessed Resazurin-based 96-well plate microdilution method. moderately inhibited both bacteria strains growing displayed antifungal effect, higher C. than parapsilosis. All these properties lead considering suitable disease prevention therapy.

Language: Английский

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