As
an
important
scavenger
of
reactive
oxygen
species,
cysteine
(Cys)
plays
a
crucial
role
in
mitochondrial
oxidative
stress.
Abnormal
intracellular
Cys
concentration
under
stress
leads
to
various
diseases.
is
also
widely
used
the
food
industry.
Many
vegetables
and
fruits
are
rich
Cys.
levels
body
can
lead
variety
Therefore,
design
fluorescent
probe
that
detect
quickly
particularly
living
organisms.
In
this
work,
novel
turn-on
mitochondrial-targeted
NIR
(SXNU-6)
was
designed
for
high
sensitivity
specificity
based
on
benzopyrylium.
Among
them,
acrylate
group
acted
as
recognition
site
fluorescence
quencher.
With
addition
Cys,
SXNU-6
gradually
enhanced
at
660
nm.
The
had
good
selectivity
sensitivity,
which
could
realize
detection
samples
(onion,
garlic,
tomato,
lettuce,
apple,
pear,
milk
bread).
Also,
both
endogenous
exogenous
LPS-induced
process.
This
work
provide
powerful
tool
detecting
biological
systems.
The
development
of
specific
and
visually
detectable
fluorophores
for
the
accurate
rapid
Pb2+
recongnization
is
crucial.
In
this
study,
we
introduced
a
novel
isophorone-derived
fluorescent
probe,
(E)-2-(3-(3-formyl-4-hydroxystyryl)-5,5-dimethylcyclohex-2-en-1-ylidene)malononitrile
(YSQ),
rationally
designed
prompt
high-quality
detection.
YSQ
demonstrated
noticeable
fluorescence
enhancement
upon
interactions,
with
visible
color
changes
discernible
to
naked
eye
in
MeOH/H2O
(v/v
9/1,
pH
7.4,
0.2
mM).
probe
achieved
quite
low
detection
limit
(LOD)
18
nM
high
association
constant
2.93
×
105
M-1
towards
using
titration.
We
proposed
binding
mechanism
ions,
supported
by
density
functional
theory
(DFT/TD-DFT),
1H
NMR
titration
experiments
IR
analysis.
addition,
simple
affordable
smartphone-supported
technique
was
developed
changes.
Furthermore,
NIR
emissive
properties
offered
broad
potential
applications
actual
water
sample
solid
analysis,
bio-imaging
test
strips.
Critical Reviews in Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 28
Published: June 5, 2024
Fluorescent
probe
technology
holds
great
promise
in
the
fields
of
environmental
monitoring
and
clinical
diagnosis
due
to
its
inherent
advantages,
including
easy
operation,
reliable
detection
signals,
fast
analysis
speed,
situ
imaging
capabilities.
In
recent
years,
a
wide
range
fluorescent
probes
based
on
diverse
fluorophores
have
been
developed
for
various
analytes,
yielding
significant
achievement.
Among
these
fluorophores,
dicyanoisophorone-based
garnered
attention.
Dicyanoisoporone
exhibits
minimal
fluorescence,
yet
possesses
robust
electron-withdrawing
capability,
rendering
it
suitable
constructing
D-π-A
structured
fluorophores.
Leveraging
intramolecular
charge
transfer
(ICT)
effect,
such
exhibit
near-infrared
(NIR)
fluorescence
emission
with
large
Stokes
shift,
thereby
offering
remarkable
advantages
design
development
NIR
probes.
This
review
article
primarily
focus
small-molecule
dicyanoisoporone-based
from
past
two
elucidating
their
strategies,
performances,
applications.
Additionally,
we
summarize
current
challenges
while
predicting
future
directions
provide
valuable
references
developing
novel
advanced
dicyanoisoporone
derivatives.
