Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 30, 2024

We present a comprehensive spectroscopic study supported by theoretical quantum chemical calculations conducted on molecular system (4-(5-methyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (C1) and the antibiotic Amphotericin B (AmB)) that exhibits highly synergistic properties. previously reported strong synergism of this now wish to related stationary measurements UV-Vis absorption, fluorescence, fluorescence anisotropy in polar, aprotic solvent (DMSO PBS buffer), followed time-resolved intensity decay studies using different ratios selected 1,3,4-thiadiazole derivative B. Absorption spectra measured for revealed discrepancies terms shapes absorption bands, particularly PBS. Fluorescence emission addition C1 molecules triggered significant changes system. Measurements lifetimes synchronous clearly showed evidence disaggregation. The AmB aggregates indicated interaction with at points responsible formation dimer structures. results were further corroborated, analyzed, interpreted methods mechanical modelling. Analyses based density functional tight-binding time-dependent theory confirmed interactions between "small" lead increase clinical efficacy antibiotic.

Language: Английский