Genome-Wide Identification and Expression Analysis of Chitinase Genes in Watermelon under Abiotic Stimuli and Fusarium oxysporum Infection

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(1), P. 638 - 638

Published: Jan. 4, 2024

Chitinases, which catalyze the hydrolysis of chitin, primary components fungal cell walls, play key roles in defense responses, symbiotic associations, plant growth, and stress tolerance. In this study, 23 chitinase genes were identified watermelon (Citrullus lanatus [Thunb.]) classified into five classes through homology search phylogenetic analysis. The with similar exon-intron structures conserved domains clustered same class. putative cis-elements involved responses to phytohormone, stress, development their promoter regions. A tissue-specific expression analysis showed that ClChi primarily expressed roots (52.17%), leaves (26.09%), flowers (34.78%). Moreover, qRT-PCR results indicate ClChis multifaceted interaction between plant/environment. More members induced by Race 2 Fusarium oxysporum f. sp. niveum, eight at higher levels on seventh day after inoculation Races 1 2, suggesting these a role resistance wilt. Collectively, improve knowledge gene family species help elucidate played chitinases various stresses.

Language: Английский

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Heat Stress and Plant–Biotic Interactions: Advances and Perspectives

Plants, Journal Year: 2024, Volume and Issue: 13(15), P. 2022 - 2022

Published: July 23, 2024

Climate change presents numerous challenges for agriculture, including frequent events of plant abiotic stresses such as elevated temperatures that lead to heat stress (HS). As the primary driving factor climate change, HS threatens global food security and biodiversity. In recent years, have negatively impacted physiology, reducing plant's ability maintain disease resistance resulting in lower crop yields. Plants must adapt their priorities toward defense mechanisms tolerate challenging environments. Furthermore, selective breeding long-term domestication higher yields made varieties vulnerable multiple stressors, making them more susceptible events. Studies on predict concurrent biotic will become severe future, potentially occurring simultaneously or sequentially. While most studies focused singular effects systems examine how plants respond specific stresses, simultaneous occurrence pose a growing threat agricultural productivity. Few explored interactions between plant-biotic interactions. Here, we aim shed light physiological molecular (bacteria, fungi, oomycetes, nematodes, insect pests, pollinators, weedy species, parasitic plants), well combined impact growth We also advances designing developing various strategies address multi-stress scenarios related factors.

Language: Английский

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Defense to Sclerotium rolfsii in groundnut (Arachis hypogaea L.) is associated with vascular tissue compactness and expression of genes coding for pathogenesis-related (PR) proteins

3 Biotech, Journal Year: 2025, Volume and Issue: 15(2)

Published: Jan. 16, 2025

Language: Английский

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Pathogenesis-Related 1 (PR1) Protein Family Genes Involved in Sugarcane Responses to Ustilago scitaminea Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6463 - 6463

Published: June 12, 2024

Plant resistance against biotic stressors is significantly influenced by pathogenesis-related 1 (PR1) proteins. This study examines the systematic identification and characterization of PR1 family genes in sugarcane (

Language: Английский

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Susceptibility assessment and genetic analysis of cucumber genotypes to damping-off disease caused by Phytophthora melonis

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102574 - 102574

Published: Jan. 1, 2025

Language: Английский

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Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 25, 2025

Reverse transcription-quantitative PCR (RT-qPCR) is a powerful tool for quantifying gene expression. However, reference genes (RGs) expression analysis in peach (Prunus persica) during interactions with Monilinia laxa, major fungal pathogen that causes brown rot, have not been established. In this study, we analysed 12 candidate RGs pathosystem by analysing samples from to 144 HAI. The stability of the was evaluated using ΔCq method and BestKeeper, NormFinder, geNorm algorithms. Our results identified AKT3, RNA pol II (RPII) SNARE (using geNorm), RPII, AKT3 TEF2 NormFinder), RPII BestKeeper) MUB6 method) as most stable mRNA normalisation across all tested samples. algorithm used determine optimal number suitable required proper under these experimental conditions, indicating three were sufficient normalisation. Analysis obtained different algorithms showed RGs. Furthermore, confirm validity genes, levels six interest, involved metabolic pathways, normalized inoculated uninoculated fruit. These findings provide set accurate RT-qPCR studies involving M. laxa interactions, facilitating deeper insights into molecular mechanisms underlying important plant–pathogen relationship.

Language: Английский

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Pilot-scale production of functionalized nanoclays for sustainable agricultural applications: process scale-up and optimization

Discover Chemical Engineering, Journal Year: 2025, Volume and Issue: 5(1)

Published: March 16, 2025

Language: Английский

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Chitinases: Key players in plant defense mechanisms against fungal pathogens

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102664 - 102664

Published: March 1, 2025

Language: Английский

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Identification of a sequence polymorphism in PST_8713 effector of Puccinia striiformis f.sp tritici and its docking with putative interacting wheat pathogenesis-related (PR) proteins

European Journal of Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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The role of salicylic acid in plant defense responses against biotic stresses

Published: Jan. 1, 2025

Language: Английский

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