International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 12266 - 12266
Published: Nov. 15, 2024
In
plants,
abscisic
acid
(ABA)
and
melatonin
(MT)
are
conventionally
treated
as
molecules
mitigating
stress
responses.
To
understand
the
mechanisms
of
ABA–MT
interplay,
we
examined
effects
ABA
MT
treatment
in
loss-of-function
mutants
Arabidopsis
thaliana
exposed
to
high
light
(HL)
stress.
constantly
suppressed
ASMT
encoding
N-acetylserotonin
methyltransferase
context
differential
responses
other
biosynthesis
genes
both
wild
type
(WT)
mutants.
However,
this
response
was
absent
mutant
with
disrupted
ABI4.
Given
that
promoter
region
contains
several
potential
ABI4-binding
elements,
these
data
suggest
can
be
a
target
gene
for
A
role
ABI4
interactions
between
is
supported
by
finding
constitutively
derepressed
signaling
cand2
gpa1,
which
exhibited
elevated
steady
state
levels
transcripts
were
not
regulated
either
or
melatonin.
addition,
abi4
showed
increased
modulations
expression
catabolic
M2H
M3H
treatment,
inferring
transcription
factor
negative
regulator
ABA-dependent
changes
content.
Furthermore,
all
tested
impaired
synthesis
displayed
compared
WT,
while
contributed
downregulation
key
genes.
Collectively,
our
results
act
antagonistically,
modulating
metabolism
interactions,
studied
severe
(SLS).
BMC Plant Biology,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: Nov. 20, 2024
Drought
stress
significantly
retards
the
plant
production.
Melatonin
is
a
vital
hormone,
signaling
molecule,
and
bio-regulator
of
diverse
physiological
growth
development
processes.
Its
role
in
boosting
agronomic
traits
under
conditions
has
received
considerable
attention.
However,
underlying
molecular
mechanism
action
how
they
increase
drought
tolerance
not
been
fully
interpreted.
The
current
study
aimed
to
ascertain
protective
melatonin
fortifying
antioxidant
defense
system,
modulating
phytohormone
profile,
improving
tomato
seedlings
stress.
After
V1
stage
(1st
leaf
emerged),
were
exposed
PEG-6000
mimic
drought-induced
(DR
10%
DR
20%),
followed
by
exogenous
application
100
µM
soil
drench.
Drought-induced
negatively
impacted
reducing
biomass
accumulation,
diminishing
salicylic
acid
(SA)
chlorophyll
levels,
dramatically
lowering
ability.
protected
them
activating
which
decreased
oxidative
burst
increased
activities
SOD,
CAT,
APX.
Administration
drench
most
remarkably
downregulated
transcription
factors
SlDREB3
SlNCED3.
This
validated
moderating
potential
against
maintaining
development,
enhancing
hormone
elevating
enzyme
activities,
suppressing
relative
expression
drought-responsive
genes.
These
findings
also
provide
basis
for
use
MT
agricultural
research
other
relevant
fields
study.
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(9), P. e0307706 - e0307706
Published: Sept. 12, 2024
Soybean
is
one
of
the
most
important
food
crops,
breeding
salt-tolerant
soybean
varieties
great
significance
to
alleviate
shortage.
In
this
study,
F-box
protein
family
homologous
gene
GmFBX322
was
cloned
from
variety
Williams
82
and
overexpressed
in
Shennong
9
further
study
explore
physiological
mechanism
salt
tolerance.
constructed
on
vector
pTF101:35S,
integrated
into
genome
by
Agrobacterium
EHA101-mediated
cotyledonary
node
transformation
technology,
4
transgenic
lines
were
obtained,
molecular
assays
performed
transformed
plants.
The
expression
detected
qRT-PCR
it
found
that
leaves
increased
2.49,
2.46,
2.77,
2.95
times
compared
with
wild
type;
after
treatment
for
12
hours,
type
Inducible
GmFBX322.
After
72
hours
treatment,
wild-type
plants
showed
obvious
wilting
chlorosis,
while
overexpressing
no
changes.
taken
at
0,
6,
12,
24,
48
stress
determine
antioxidant
activity.
Ability
osmotic
adjustment
level,
etc.
results
catalase
activity
2265,
2267,
2269,
2271
2.47,
2.53,
3.59,
2.96
plant
treatment;
soluble
sugar
content
1.22,
1.14,
1.22
plant.
1.57
times;
proline
2.20,
1.83,
1.65,
1.84
type.
comparing
indicators
determined
experiment,
better
than
control
group,
indicating
overexpression
can
enhance
tolerance
To
verify
function
related
resistance,
add
candidate
provide
scientific
basis
selection
varieties.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(22), P. 12266 - 12266
Published: Nov. 15, 2024
In
plants,
abscisic
acid
(ABA)
and
melatonin
(MT)
are
conventionally
treated
as
molecules
mitigating
stress
responses.
To
understand
the
mechanisms
of
ABA–MT
interplay,
we
examined
effects
ABA
MT
treatment
in
loss-of-function
mutants
Arabidopsis
thaliana
exposed
to
high
light
(HL)
stress.
constantly
suppressed
ASMT
encoding
N-acetylserotonin
methyltransferase
context
differential
responses
other
biosynthesis
genes
both
wild
type
(WT)
mutants.
However,
this
response
was
absent
mutant
with
disrupted
ABI4.
Given
that
promoter
region
contains
several
potential
ABI4-binding
elements,
these
data
suggest
can
be
a
target
gene
for
A
role
ABI4
interactions
between
is
supported
by
finding
constitutively
derepressed
signaling
cand2
gpa1,
which
exhibited
elevated
steady
state
levels
transcripts
were
not
regulated
either
or
melatonin.
addition,
abi4
showed
increased
modulations
expression
catabolic
M2H
M3H
treatment,
inferring
transcription
factor
negative
regulator
ABA-dependent
changes
content.
Furthermore,
all
tested
impaired
synthesis
displayed
compared
WT,
while
contributed
downregulation
key
genes.
Collectively,
our
results
act
antagonistically,
modulating
metabolism
interactions,
studied
severe
(SLS).
