Bacillus velezensis LMY3-5 for the biocontrol of soft rot in kiwifruit: antifungal action and underlying mechanisms DOI Creative Commons
Chunguang Ren, Yu Liu,

Wenwen Su

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Soft rot caused by Botryosphaeria dothidea is a serious fungal disease in kiwifruit observed during the storage period, and it seriously restricts healthy stable development of industry. In present study, bacterial strain LMY3-5 with high antifungal activity was isolated from tissues. Based on gyrA 16S rRNA sequences, phylogenetic tree constructed, identified as Bacillus velezensis . The 16% cell-free supernatant (CFS) suppressed B. spore germination mycelial growth 97.32%. CFS could effectively inhibit expansion soft vitro , inhibition rate 73.59%. scanning electron microscopy (SEM) observations mycelia treated for 12 h showed that were curved, wrinkled, sunken. Moreover, transmission (TEM) revealed blurred boundaries cell walls well plasmolysis vacuolization. Propidium iodide (PI) staining treatment damaged membrane affected its permeability, which led to leakage nucleic acids proteins. Simultaneously, substantial increase key enzymes (i.e., β -1,3-glucanase chitinase) observed, indicated damage wall function dothidea. GC/LC–MS analysis presence 27 antimicrobial compounds. Thus, great potential biocontrol agent kiwifruit.

Language: Английский

Strategies to Delay Ethylene-Mediated Ripening in Climacteric Fruits: Implications for Shelf Life Extension and Postharvest Quality DOI Creative Commons
Ramiro Alonso-Salinas, Santiago López‐Miranda, Antonio J. Pérez-López

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 840 - 840

Published: Aug. 8, 2024

Climacteric fruits undergo a characteristic ripening process regulated by ethylene, key plant hormone. Extending the shelf life of these while preserving their postharvest quality poses significant challenge for food industry. This review provides comprehensive overview physiological and molecular strategies to delay ethylene-mediated in climacteric impact on life, quality, sensory attributes, volatile compounds. Additionally, it examines role ethylene fruit ripening, analysing various managing including inhibitors, adsorbents, scavengers catalytic oxidation. concludes with future research directions genetic approaches reducing production or responsiveness fruits, integrated strategies, environmental considerations, commercial applications improving handling quality.

Language: Английский

Citations

4
Bacillus velezensis LMY3-5 for the biocontrol of soft rot in kiwifruit: antifungal action and underlying mechanisms DOI Creative Commons
Chunguang Ren, Yu Liu,

Wenwen Su

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Soft rot caused by Botryosphaeria dothidea is a serious fungal disease in kiwifruit observed during the storage period, and it seriously restricts healthy stable development of industry. In present study, bacterial strain LMY3-5 with high antifungal activity was isolated from tissues. Based on gyrA 16S rRNA sequences, phylogenetic tree constructed, identified as Bacillus velezensis . The 16% cell-free supernatant (CFS) suppressed B. spore germination mycelial growth 97.32%. CFS could effectively inhibit expansion soft vitro , inhibition rate 73.59%. scanning electron microscopy (SEM) observations mycelia treated for 12 h showed that were curved, wrinkled, sunken. Moreover, transmission (TEM) revealed blurred boundaries cell walls well plasmolysis vacuolization. Propidium iodide (PI) staining treatment damaged membrane affected its permeability, which led to leakage nucleic acids proteins. Simultaneously, substantial increase key enzymes (i.e., β -1,3-glucanase chitinase) observed, indicated damage wall function dothidea. GC/LC–MS analysis presence 27 antimicrobial compounds. Thus, great potential biocontrol agent kiwifruit.

Language: Английский

Citations

0