Natural allelic variation of NAC transcription factor 22 regulates starch biosynthesis and properties in sweetpotato DOI
Yue Fan, Luyao Xue,

M. Shang

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

ABSTRACT Sweetpotato ( Ipomoea batatas ) starch is in high demand globally as a food and industrial product. However, the regulatory mechanisms governing biosynthesis properties this important crop remain largely unknown. Here we identified natural allelic variant promoter of IbNAC22 , encoding NAC (NAM, ATAF1/2, CUC2) transcription factor, which closely linked to content sweetpotato. In high‐starch sweetpotato varieties, T/C haplotype 13‐bp deletion resulted higher transcriptional activity. The IbNAC 22 more prevalent regions China where industry well developed, indicating that advantageous allele type has been utilized breeding starchy varieties China. highly expressed storage roots starch‐rich accessions. Overexpression significantly improved amylose contents, granule size gelatinization temperature, decreased crystallinity, whereas knockdown had opposite effects. directly activates expression IbGBSSI key gene for biosynthesis, but suppresses IbSBEI amylopectin biosynthesis. interacts with IbNF‐YA10. Overexpressing IbNF‐YA10 size, chain length distribution. IbAGPL are genes involved IbNAC22–IbNF‐YA10 heterodimers further enhance IbNF‐YA10‐induced activation . These findings increase our understanding provide strategies candidate improvement root tuber crops.

Language: Английский

Unique starch biosynthesis pathways in wild rice revealed by multi‐omics analyses DOI Creative Commons

Nurmansyah NURMANSYAH,

Agnelo Furtado, Pauline Okemo

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Australian wild rice species (AWS) possess unique starch properties characterized by a slow digestibility rate. However, the genomic and transcriptomic variations of starch-synthesis-related genes (SSRGs) influencing physiochemical in AWS remain unclear. Here, we report comparative analyses 72 SSRGs species, including two (O. meridionalis populations O. rufipogon) domesticated gene pool. Our findings reveal that most are more actively expressed early stages seed development. Transcriptome analysis identified differential splicing patterns, with synthesis pathways Nipponbare rufipogon being similar than those meridionalis. Three essential genes, GBSSI, SSIIa BEIIb, were active had higher expression compared to Nipponbare, explaining amylose content, gelatinization temperature, soft gel consistency high retrogradation rice. Comparative genomics indicated Asian evolved from single ancestral allele GBSSI (Wxlv) (ALKc), but BEIIb alleles originated nivara, considered progenitors Additionally, expressions BEI SSIIIa contribute slower rate, making its haplotypes valuable for breeding develop slowly digested cultivars. These not only provide insight into evolution during domestication also pave way unlocking desirable improve quality cultivated

Language: Английский

Citations

0
Natural allelic variation of NAC transcription factor 22 regulates starch biosynthesis and properties in sweetpotato DOI
Yue Fan, Luyao Xue,

M. Shang

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

ABSTRACT Sweetpotato ( Ipomoea batatas ) starch is in high demand globally as a food and industrial product. However, the regulatory mechanisms governing biosynthesis properties this important crop remain largely unknown. Here we identified natural allelic variant promoter of IbNAC22 , encoding NAC (NAM, ATAF1/2, CUC2) transcription factor, which closely linked to content sweetpotato. In high‐starch sweetpotato varieties, T/C haplotype 13‐bp deletion resulted higher transcriptional activity. The IbNAC 22 more prevalent regions China where industry well developed, indicating that advantageous allele type has been utilized breeding starchy varieties China. highly expressed storage roots starch‐rich accessions. Overexpression significantly improved amylose contents, granule size gelatinization temperature, decreased crystallinity, whereas knockdown had opposite effects. directly activates expression IbGBSSI key gene for biosynthesis, but suppresses IbSBEI amylopectin biosynthesis. interacts with IbNF‐YA10. Overexpressing IbNF‐YA10 size, chain length distribution. IbAGPL are genes involved IbNAC22–IbNF‐YA10 heterodimers further enhance IbNF‐YA10‐induced activation . These findings increase our understanding provide strategies candidate improvement root tuber crops.

Language: Английский

Citations

0