Cinnamomum burmannii Essential Oil as a Promising Antimicrobial Agent Against Cutaneous Pathogens: Mechanistic Insights into Its Anti-Malassezia furfur Activity DOI Creative Commons
Wenwen Wang,

Shikai Cai,

Ying Wang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(6), P. 1241 - 1241

Published: May 28, 2025

This study investigated the chemical composition, antibacterial activity and antifungal mechanisms of Cinnamomum burmannii essential oil (CBEO) obtained from leaves branches through pilot-scale steam molecular distillation after D-borneol crystallization, focusing on its inhibitory effects against Malassezia furfur (M. furfur). GC-MS analysis identified 78 constituents in CBEO, with monoterpenoid predominating. CBEO exhibited potent M. furfur, MIC MFC values 0.88 mg/mL 1.75 mg/mL, respectively. Synergistic were observed when combined ketoconazole (FICI = 0.5). At 2 × concentration, suppressed 85.6% biofilm formation (p < 0.01) as determined by crystal violet assay. SEM imaging revealed that treatment induced surface invaginations pore structures fungal cells. Quantitative detection intracellular protein, nucleic acid, ion leakage levels confirmed enhanced membrane permeability, resulting cytoplasmic content leakage. Ergosterol binding assays cell disruption (8-fold increase), while UPLC quantification demonstrated dose-dependent suppression ergosterol synthesis. Correspondingly, squalene epoxidase (SE) was significantly inhibited treated These findings systematically elucidate CBEO’s anti-M. mechanisms, highlighting potential a natural agent for cosmeceutical applications.

Language: Английский

Cinnamomum burmannii Essential Oil as a Promising Antimicrobial Agent Against Cutaneous Pathogens: Mechanistic Insights into Its Anti-Malassezia furfur Activity DOI Creative Commons
Wenwen Wang,

Shikai Cai,

Ying Wang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(6), P. 1241 - 1241

Published: May 28, 2025

This study investigated the chemical composition, antibacterial activity and antifungal mechanisms of Cinnamomum burmannii essential oil (CBEO) obtained from leaves branches through pilot-scale steam molecular distillation after D-borneol crystallization, focusing on its inhibitory effects against Malassezia furfur (M. furfur). GC-MS analysis identified 78 constituents in CBEO, with monoterpenoid predominating. CBEO exhibited potent M. furfur, MIC MFC values 0.88 mg/mL 1.75 mg/mL, respectively. Synergistic were observed when combined ketoconazole (FICI = 0.5). At 2 × concentration, suppressed 85.6% biofilm formation (p < 0.01) as determined by crystal violet assay. SEM imaging revealed that treatment induced surface invaginations pore structures fungal cells. Quantitative detection intracellular protein, nucleic acid, ion leakage levels confirmed enhanced membrane permeability, resulting cytoplasmic content leakage. Ergosterol binding assays cell disruption (8-fold increase), while UPLC quantification demonstrated dose-dependent suppression ergosterol synthesis. Correspondingly, squalene epoxidase (SE) was significantly inhibited treated These findings systematically elucidate CBEO’s anti-M. mechanisms, highlighting potential a natural agent for cosmeceutical applications.

Language: Английский

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