Miniaturized Analytical Strategy Based on μ-SPEed for Monitoring the Occurrence of Pyrrolizidine and Tropane Alkaloids in Honey

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 72(1), P. 819 - 832

Published: Dec. 18, 2023

Currently, the analysis of trace-level contaminants in food must be addressed following green analytical chemistry principles and with a commitment to sustainable development goals. Accordingly, ecofriendly microextraction procedure based on μ-SPEed followed by ultrahigh liquid chromatography coupled ion-trap tandem mass spectrometry was developed determine occurrence pyrrolizidine tropane alkaloids honey samples. The took approximately 3 min per sample, using only 100 μL organic solvent 300 diluted sample. method properly validated (overall recoveries 72–100% precision RSD values ≤15%), its greenness scored at 0.61 out 1. applied different samples, showing overall contamination levels from 32 177 μg/kg these alkaloids. Atropine found all whereas retrorsine N-oxide, lasiocarpine, echimidine, echimidine N-oxide were main samples analyzed.

Language: Английский

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Antioxidant activity and total phenolic compounds of Commiphora gileadensis extracts obtained by ultrasonic‐assisted extraction, with monitoring antiaging and cytotoxicity activities

Food Science & Nutrition, Journal Year: 2023, Volume and Issue: 11(6), P. 3506 - 3515

Published: April 5, 2023

Commiphora gileadensis (C. gileadensis) has been identified and linked with various health benefits pharmaceutical potential for its phytochemical activities chemical constituents. This study aimed to evaluate ultrasonic-assisted extraction (USE) technique total phenols content from C. leaf compared the hydrodistillation (HDE). Our results showed that USE operating conditions were as: MeOH·H2O solvent-to-fresh sample ratio of 80:20 (v/v); ultrasonic power/frequency at 150 W/20 kHz; a temperature 40 ± 1°C; subjected acoustic waves intermittently calculated time (5 min) during programmed 12 min. The exhibited (118.71 0.009 mg GAE/g DM) more amounts all than HDE (101.47 0.005 DM), antioxidant (77.78 0.73%, 75.27 0.59% scavenging inhibition DPPH), respectively. Anti-aging Cytotoxicity investigated. biological evaluations crude extracts significantly extended replicative lifespan K6001 yeast. In addition, in vitro cytotoxicity against HepG2 cell line significant anticancer activity, approximately 100 μg/mL is required decrease viability control. proven larger scale extract isolate compounds utilization industry. conclusion, advanced methods afford an high activity properties extract.

Language: Английский

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Novel Sustainable Alternatives for the Study of the Chemical Composition of Cork

Sustainability, Journal Year: 2024, Volume and Issue: 16(2), P. 575 - 575

Published: Jan. 9, 2024

Cork is a natural and renewable material extracted from the cork oak Quercus suber L. (1) Background: The cellular structure chemical composition of plant wall give its physical mechanical properties. Chemically, principally dominated by presence suberin as main structural cell component affected close association with other components. usual analysis has been partially adapted wood lignocellulosic analytical methods it method extensive procedures. This study aims to (i) find quicker more sustainable for testing composition, (ii) conduct extraction tests using alternative techniques, (iii) optimize methodologies, (iv) validate proposed methodologies. (2) Methods: Microwave-assisted (MAE) accelerated solvent (ASE) are explored systems traditional method. percentages extractives obtained via MAE lower than means classical composition. (3) Results: However, very similar in both methods: 46.39% case 45.11% microwave-assisted extraction. No significant differences observed between content dichloromethane, ethanol, water ASE results methodology. (4) Conclusions: faster methods; they use less solvents provide reproducible These data pave way novel alternatives relative studies cork.

Language: Английский

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Quantitative analysis and health risk assessment of selected heavy metals in pet food samples using ultrasound assisted hydrogen peroxide extraction followed by ICP-OES analysis

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 192, P. 114915 - 114915

Published: Aug. 9, 2024

There is a lack of information regarding the presence heavy metals in feed ingredients for animals. Therefore, this study examines 10 samples collected from commercial pet food South African market. The optimal working parameters ultrasound assisted hydrogen peroxide extraction (UA-HPE) confirmed by multivariate optimization were sonication temperature at 80 °C 60 min, sample mass 0.1 g, and H

Language: Английский

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Recent Progress of Electrochemical Aptasensors toward AFB1 Detection (2018–2023)

Biosensors, Journal Year: 2023, Volume and Issue: 14(1), P. 7 - 7

Published: Dec. 22, 2023

Food contaminants represent possible threats to humans and animals as severe food safety hazards. Prolonged exposure contaminated often leads chronic diseases such cancer, kidney or liver failure, immunosuppression, genotoxicity. Aflatoxins are naturally produced by strains of the fungi species

Language: Английский

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