Enhanced identification of glycoproteins and allergens in Hevea brasiliensis latex using innovative enrichment techniques

Preparative Biochemistry & Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 9

Published: Feb. 4, 2025

Hevea brasiliensis is the most efficient crop for latex production and has been main source global commercialisation since 1876. However, little known about its glycosylation properties. Studying glycoproteomics challenging due to analytical difficulties heterogeneous nature of glycans. In this study, we extracted natural rubber (NRL) proteins, purify peptide/glycopeptide mixtures isolate glycopeptides using HILIC, SAX, TiO2 enrichment methods. showed high selectivity compared SAX retaining unique proteins glycans based on hydrophilicity. We identified LC-MS/MS PEAKS Studio 7.5. These methods significantly improved protein coverage non-enriched samples. Three glycoproteins (patatin, REF, glucan endo-1,3-beta-D-glucosidase) seven internationally recognised allergens (hev b 1, hev 2, 3, 5, 7, 14, 15) were identified. not only helped identify but also sequence coverage.

Language: Английский

Sample preparation for glycoproteins

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский