Spectroscopic Visualization of Drug–Biomolecules Interactions: An Insight to Fluorescence Quenching as Tool in Drug Discovery

Luminescence, Journal Year: 2025, Volume and Issue: 40(4)

Published: April 1, 2025

ABSTRACT Fluorescence quenching, a process where the intensity of fluorescence is diminished by various molecular interactions, has emerged as critical tool in drug discovery. This review delves into underlying mechanisms including static and dynamic Förster resonance energy transfer (FRET), photoinduced electron (PET). Each mechanism offers unique insights binding affinities, conformational changes candidates, enabling researchers to dissect complex biological systems with precision. The application quenching high‐throughput screening (HTS) particularly emphasized, highlighting its role identifying lead compounds optimizing drug–target interactions. Furthermore, explores integration advanced techniques, such time‐resolved single‐molecule spectroscopy, elucidating phenomena at level. These techniques provide deeper understanding drug–receptor allosteric modulation, protein dynamics, which are pivotal development pipeline. potential probing pharmacokinetics pharmacodynamics novel therapeutics also discussed, underscoring versatility effectiveness. By offering comprehensive analysis their applications, this aims inform future discovery endeavors, fostering more effective targeted therapies.

Language: Английский

Editorial overview: New perspectives on the structure and dynamics of protein-nucleic acid interactions

Current Opinion in Structural Biology, Journal Year: 2024, Volume and Issue: 90, P. 102957 - 102957

Published: Dec. 3, 2024

Language: Английский