Explore autophagy-related lncRNA-miRNA-mRNA ceRNA networks for diagnosis of early-onset schizophrenia through transcriptome analysis DOI Creative Commons
Wei Hu,

Xinzhe Du,

Xinxia Wang

et al.

Frontiers in Psychiatry, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

The severe functional impairment and poor prognosis of early-onset schizophrenia (EOS) create a great need to identify effective biomarkers for early diagnosis in young psychiatric patients. Current research indicates potential link between loss autophagy function emotional behavioral abnormalities individuals with disorders. This study aimed explore diagnostic autophagy-related endogenous competitive RNA (ceRNA) networks EOS messenger RNAs (mRNAs) long non-coding (lncRNAs) expression profiles were obtained from peripheral blood mononuclear cells 18 patients 12 healthy controls (HC). A co-expression analysis was performed 365 core lncRNAs 55 differentially expressed genes (ARGs) lncRNAs. Subsequently, five identified as candidate construct ceRNA regulatory network using least absolute shrinkage selection operator (LASSO) Cox regression, receiver operating characteristic (ROC) curve evaluate their predictive accuracy. Then, putative interactions among lncRNA-microRNAs (miRNAs)-mRNA determined based on the lncRNASNP2 TarBase databases. Three lncRNAs, twenty miRNAs, ten mRNAs selected an autophagy-associated associated occurrence. Through protein-protein interaction analysis, hub identified, which exhibited good ability distinguishing individuals. ROC demonstrated that integrating three (RP1-135L22.1, RP5-884C9.2, RP11-390F4.3) along (EIF4G1, AKT1, BAX, WIPI2, MAPT) appeared yield better accuracy compared either or alone. Furthermore, all positively correlated at two types immune infiltration. transcriptome we searched networks, provided valuable candidates EOS.

Language: Английский

An RB1CC1 Missense Variant in Nova Scotia Duck Tolling Retrievers with Degenerative Encephalopathy DOI Open Access

Juyuan Guo,

Garrett Bullock,

Dennis P. O’Brien

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 269 - 269

Published: Feb. 25, 2025

Background/Objectives: A slowly progressive hereditary neurological disorder classified as degenerative encephalopathy (DE) occurs in Nova Scotia Duck Tolling Retrievers. The disease is characterized by frequent episodes of pronounced involuntary movements during sleep, cognitive impairment, anxiety, heightened sensitivity to sensory stimuli, and compulsive behaviors. clinical signs are accompanied the degeneration several brain regions. study was undertaken identify molecular genetic basis this disorder. Methods: Whole genome sequences (WGSs) from DNA affected unaffected Retrievers were aligned Dog10K_Boxer_Tasha reference assembly WGSs 334 additional control dogs generated laboratory. Results: missense C>T variant identified RB1CC1 exon 22 chromosome 29:4891014 that uniquely homozygous dog. This predicts a p.G1503R change amino acid sequence RB1CC1. Genotyping 2950 at locus found complete concordance between phenotype genotype. Conclusions: RBCC1 an essential component protein complex mediates initiation autophagosome formation. Therefore, it appears likely results, least part, impaired autophagy. Consistent with possibility, neurons dog contain abnormal lysosomal storage body-like inclusions. could serve valuable model elucidate mechanisms underlying human diseases associated Identification disease-causing will enable owners screen their for risk variant.

Language: Английский

Citations

0
Explore autophagy-related lncRNA-miRNA-mRNA ceRNA networks for diagnosis of early-onset schizophrenia through transcriptome analysis DOI Creative Commons
Wei Hu,

Xinzhe Du,

Xinxia Wang

et al.

Frontiers in Psychiatry, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

The severe functional impairment and poor prognosis of early-onset schizophrenia (EOS) create a great need to identify effective biomarkers for early diagnosis in young psychiatric patients. Current research indicates potential link between loss autophagy function emotional behavioral abnormalities individuals with disorders. This study aimed explore diagnostic autophagy-related endogenous competitive RNA (ceRNA) networks EOS messenger RNAs (mRNAs) long non-coding (lncRNAs) expression profiles were obtained from peripheral blood mononuclear cells 18 patients 12 healthy controls (HC). A co-expression analysis was performed 365 core lncRNAs 55 differentially expressed genes (ARGs) lncRNAs. Subsequently, five identified as candidate construct ceRNA regulatory network using least absolute shrinkage selection operator (LASSO) Cox regression, receiver operating characteristic (ROC) curve evaluate their predictive accuracy. Then, putative interactions among lncRNA-microRNAs (miRNAs)-mRNA determined based on the lncRNASNP2 TarBase databases. Three lncRNAs, twenty miRNAs, ten mRNAs selected an autophagy-associated associated occurrence. Through protein-protein interaction analysis, hub identified, which exhibited good ability distinguishing individuals. ROC demonstrated that integrating three (RP1-135L22.1, RP5-884C9.2, RP11-390F4.3) along (EIF4G1, AKT1, BAX, WIPI2, MAPT) appeared yield better accuracy compared either or alone. Furthermore, all positively correlated at two types immune infiltration. transcriptome we searched networks, provided valuable candidates EOS.

Language: Английский

Citations

0