Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100797 - 100797
Published: April 1, 2025
Language: Английский
BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 14, 2025
The emergence and prevalence of hypervirulent Klebsiella pneumoniae (hvKP) have proposed a great challenge to control this infection. Therefore, exploring some new drugs or strategies for treating hvKP infection is an urgent issue scientific researchers. In the present study, clpV gene deletion strain (ΔclpV-hvKP) was constructed using CRISPR-Cas9 technology, biological characteristics ΔclpV-hvKP were investigated explore targets controlling pathogen. results showed that did not affect growth ability hvKP. However, knocking out markedly decreased mucoid phenotype biofilm formation It reduced interspecific competition with Escherichia coli, Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus. significantly changed transcriptome profile hvKP, inhibited expression virulence factors, lethality against Galleria mellonella larvae. vitro experiments lithocholic acid could inhibit reduce Our data suggested may be potential target decreasing risk.
Language: Английский
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Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 647 - 660
Published: Feb. 1, 2025
Purpose: This study aims to investigate the relationship between glycemic control and epidemiological characteristics of patients infected with carbapenem-resistant Klebsiella pneumoniae (CRKP), identify mortality risk factors associated CRKP infection, evaluate impact glucose on resistance polymyxin serum killing. Patients Methods: Clinical cases 218 were collected from a large tertiary public hospital in Anhui Province. We analyzed whether impacts clinical laboratory manifestations patients. Logistic regression identified factors. Antibiotic sensitivity, capsular serotypes, virulence genes tested strains. Three clinically isolated strains used effect bacterial capsule synthesis Results: poor experienced more severe infections had higher likelihood chronic kidney disease (CKD) acute renal insufficiency compared those good control. They also exhibited an increased rate. analysis age, glycosylated hemoglobin (HbA 1c ) ≥ 7%, CKD, tumor, mechanical ventilation, sepsis as independent for death infection. A 0.5% (0.5 g/100mL) environment can stimulate synthesis, which is inhibitable by cyclic adenosine monophosphate (cAMP). Moreover, high-glucose enhance CRKP's Conclusion: persistent hyperglycemic resulting may capsules, could killing, thereby further increasing patient mortality. Keywords: , control, polymyxin, resistance, capsule, diabetes
Language: Английский
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Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: 230, P. 234 - 247
Published: Feb. 16, 2025
Language: Английский
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0
Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 24, 2025
Klebsiella pneumoniae is an opportunistic pathogen primarily associated with nosocomial infections, characterized by a propensity for multi-drug resistance and the potential evolution into hypervirulent strains. Based on its phenotypic genotypic characteristics, K. can be classified two types: classical (cKP) (hvKP). The spread of mobile genetic elements (MGEs) in has led to emergence carbapenem-resistant (CRKP) (CR-hvKP). CR-hvKP particularly concerning due multidrug resistance, high pathogenicity, increased transmissibility. This review summarizes types MGEs present pneumoniae, mechanisms horizontal gene transfer (HGT) mediated these elements, their roles dissemination antibiotic genes (ARGs) virulence genes, relationships among that resemble Russian dolls or exhibit hybrid characteristics. Additionally, clinical treatment epidemiological characteristics are discussed. Given variability transmissibility MGEs, continuous monitoring control variation transmission such material should prioritized.
Language: Английский
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Journal of Microbiology Immunology and Infection, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance hypervirulence phenotypes, but the differences in epidemiological, phenotypic genotypic characteristics between them remains unclear. In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates a tertiary hospital Shanghai, China from January 2019 to December 2022. Furthermore, selected four typical hypervirulent carbapenem-resistant Klebsiella pneumoniae, including ST23-KL1/ST86-KL2 (WYKP3 WYKP194) ST11-KL64/ST11-KL47 (WYKP589 WYKP188), tried clarify compare their virulence drug plasmid distribution. Our study found that ST23-KL1 ST86-KL2 exhibited less diversity than of ST11-KL64 ST11-KL47 hv-CRKP. Compared with hv-CRKP, harbored significantly fewer antimicrobial genes more genes, which contributed higher these strains antibiotics. has emerged as most prevalent pneumoniae probably due its clonal transmission within hospitals well plasmids help conjugative plasmids. Due different evolutionary mechanisms capsular serotypes, are different.
Language: Английский
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Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16
Published: March 7, 2025
Introduction Hypervirulent and multidrug-resistant Klebsiella pneumoniae (hvKP MDR-KP) are significant public health threats. This study aimed to isolate a lytic bacteriophage targeting these high-risk strains, systematically characterize its biological properties, genomic features, therapeutic efficacy, establish foundation for clinical phage therapy novel antimicrobial development. Methods The vB_Kp_XP4 was isolated from river water using the double-layer agar plate method with clinically strain P4 as host. Morphology analyzed via transmission electron microscopy (TEM). Host range, pH, thermal stability were assessed spot assays OD 630 measurements. One-step growth curves determined latent period burst size. Whole-genome sequencing phylogenetic analysis performed. Therapeutic efficacy safety evaluated in Galleria mellonella infection model. Results TEM revealed Phage tailed an icosahedral head long, flexible tail. It lysed hvKP (carrying rmp , peg iuc iro genes) MDR-KP (resistant carbapenems, fluoroquinolones, etc.), optimal MOI of 0.1 <10 minutes. Stability maintained at pH 4–11 ≤70°C. linear double-stranded DNA genome 44,344 bp G+C content 53.80%. comprised 54 coding sequences lacked lysogenic, virulence, or antibiotic resistance genes. Phylogenetic positioned species within genus Drulisvirus family Autographiviridae . In model, prolonged survival P4-infected larvae ( P < 0.001) Conclusion exhibits high stability, specificity, potent activity, no undesirable genes, demonstrating effective vivo suggest potential applications against infections. presence multiple halos during plaque formation further enhances research value. complete sequence has been submitted GenBank under accession number PP663283.
Language: Английский
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EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105649 - 105649
Published: March 10, 2025
Language: Английский
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Multidisciplinary Reviews, Journal Year: 2025, Volume and Issue: 8(7), P. 2025211 - 2025211
Published: Feb. 6, 2025
Superbugs, or multidrug-resistant bacteria, represent a growing threat to global health, particularly in paediatric populations. The inappropriate and excessive use of antibiotics exerts selective pressure on bacterial populations, promoting the survival resistant strains while eliminating susceptible ones. Superbugs are dangerous pediatric where infections with bacteria result higher morbidity mortality rates. Misuse over-prescription continue be key contributors emergence strains. Children especially vulnerable due their developing immune systems frequent exposure healthcare-associated infections. This review focuses most common superbugs affecting children, including Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, Carbapenem-resistant Enterobacteriaceae (CRE), Pseudomonas aeruginosa (MDRPA). examines recent studies prevalence, clinical impact, treatment challenges posed by these pathogens patients. MRSA continues leading cause skin, soft tissue, bone infections, VRE is increasingly seen nosocomial rise ESBL-producing complicates management urinary tract non-carbapenem treatments like gentamicin nitrofurantoin showing promise. CRE MDRPA critically ill associated high rates limited options. Newer such as daptomycin, linezolid, ceftazidime-avibactam offer potential, but concerns regarding side effects lack paediatric-specific data persist. Effective children requires ongoing surveillance, judicious antibiotics, continued research develop safer more effective therapies. underscores urgent need for research, combat rising
Language: Английский
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Future Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13
Published: March 26, 2025
Multidrug-resistant hypervirulent Klebsiella pneumoniae (MDR-hvKP) combines high pathogenicity with multidrug resistance to become a new superbug. MDR-hvKP reports continue emerge, shattering the perception that K. (hvKP) strains are antibiotic sensitive. Patients infected have been reported in Asia, particularly China. Although hvKP can acquire drug genes, seems be more easily transformed from classical (cKP), which has strong gene uptake ability. To better understand biology of MDR-hvKP, this review discusses virulence factors, mechanisms, formation pathways, and identification MDR-hvKP. Given their destructive transmissible potential, continued surveillance these organisms enhanced control measures should prioritized.
Language: Английский
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Chemical Engineering Science, Journal Year: 2025, Volume and Issue: unknown, P. 121628 - 121628
Published: April 1, 2025
Language: Английский
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0