Soil
improvement
methods
can
result
in
changes
the
microbial
community
blueberry
soil.
However,
there
have
been
few
reports
on
impacts
of
different
soil
function,
particularly
endophytic
microbe.
In
this
study,
we
analyzed
response
composition,
function
and
nitrogen
(N)
cycle
to
using
high
throughput
sequencing.
We
aimed
investigate
best
method
from
a
perspective.
The
results
showed
that
highest
diversity
was
observed
T4
treatment
(peat
combined
with
mushroom
bran),
followed
by
T2
acidified
rice
husk)
both
rhizosphere
roots.
dominant
phyla
were
Proteobacteria
Actinobacteria,
which
most
abundance
T1
sulfur)
treatments
soil,
respectively,
but
opposite
trend
root
bacterial
community.
Interestingly,
Acidobacterium
Paludibaculum,
belonging
Acidobacteria
phylum,
found
influence
according
correlation
network
analysis.
And
these
bacteria
abundant
communities
clustered
into
two
categories:
one
for
treatments,
another
T3
(mushroom
bran)
treatments.
Compared
other
had
significant
impact
functional
pathways
promoted
growth
N
fixation
At
module
level,
increased
relative
decreased
assimilating
nitrate
reduction
reaction
(ANRA),
dissimilating
(DNRA),
denitrification
completed
nitrification
Additionally,
microbes
involved
fixation.
Overall,
our
findings
suggest
addition
peat
husk
is
optimal
cultivation.
Plants,
Journal Year:
2024,
Volume and Issue:
13(7), P. 1042 - 1042
Published: April 8, 2024
The
remediation
of
copper
and
nickel-afflicted
sites
is
challenged
by
the
different
physiological
effects
imposed
each
metal
on
a
given
plant
system.
Pinus
banksiana
resilient
against
nickel,
providing
an
opportunity
to
build
valuable
resource
investigate
responding
gene
expression
toward
metal.
objectives
this
study
were
(1)
extend
analysis
transcriptome
exposed
nickel
copper,
(2)
assess
differential
in
nickel-resistant
compared
copper-resistant
genotypes,
(3)
identify
mechanisms
specific
Illumina
platform
was
used
sequence
RNA
that
extracted
from
seedlings
treated
with
metals.
There
449
differentially
expressed
genes
(DEGs)
between
genotypes
(RGs)
at
high
stringency
cut-off,
indicating
distinct
pattern
For
biological
processes,
19.8%
DEGs
associated
DNA
metabolic
process,
followed
response
stress
(13.15%)
chemicals
(8.59%).
function,
27.9%
nuclease
activity,
nucleotide
binding
(27.64%)
kinase
activity
(10.16%).
Overall,
21.49%
localized
plasma
membrane,
cytosol
(16.26%)
chloroplast
(12.43%).
Annotation
top
upregulated
RG
identified
not
nickel.
NtPDR,
AtHIPP10,
YSL1
as
resistance.
Various
related
cell
wall
metabolism
identified,
they
included
encoding
for
HCT,
CslE6,
MPG,
polygalacturonase.
downregulated
revealed
copper.
regulatory
signaling-related
identified.
They
UGT,
TIFY,
ACC,
dirigent
protein,
peroxidase,
glyoxyalase
I.
Additional
research
needed
determine
functions
signaling
plants.
MicroRNAs
(MiRNAs)
are
a
class
of
non-coding
single-stranded
RNA
molecules
approximately
20-24
nucleotides
in
plants
that
play
an
important
regulatory
role
variety
biological
processes
such
as
plant
growth
and
development
response
to
various
abiotic
stresses.
For
example
Drought,
Salt,
Cold,
High
temperature,
Heavy
metals
Nutrition.
MiRNAs
affect
gene
expression
by
manipulating
the
cleavage,
translational
or
DNA
methylation
target
mRNAs.
This
review
describes
current
progress
made
on
way
miRNAs
produced
regulated
miRNA/target
is
used
responses
Studying
molecular
mechanism
action
downstream
genes
can
optimize
genetic
manipulation
crop
conditions
provide
more
theoretical
basis
for
improving
production.
Soil
improvement
methods
can
result
in
changes
the
microbial
community
blueberry
soil.
However,
there
have
been
few
reports
on
impacts
of
different
soil
function,
particularly
endophytic
microbe.
In
this
study,
we
analyzed
response
composition,
function
and
nitrogen
(N)
cycle
to
using
high
throughput
sequencing.
We
aimed
investigate
best
method
from
a
perspective.
The
results
showed
that
highest
diversity
was
observed
T4
treatment
(peat
combined
with
mushroom
bran),
followed
by
T2
acidified
rice
husk)
both
rhizosphere
roots.
dominant
phyla
were
Proteobacteria
Actinobacteria,
which
most
abundance
T1
sulfur)
treatments
soil,
respectively,
but
opposite
trend
root
bacterial
community.
Interestingly,
Acidobacterium
Paludibaculum,
belonging
Acidobacteria
phylum,
found
influence
according
correlation
network
analysis.
And
these
bacteria
abundant
communities
clustered
into
two
categories:
one
for
treatments,
another
T3
(mushroom
bran)
treatments.
Compared
other
had
significant
impact
functional
pathways
promoted
growth
N
fixation
At
module
level,
increased
relative
decreased
assimilating
nitrate
reduction
reaction
(ANRA),
dissimilating
(DNRA),
denitrification
completed
nitrification
Additionally,
microbes
involved
fixation.
Overall,
our
findings
suggest
addition
peat
husk
is
optimal
cultivation.
