Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 17, 2024
AbstractHymenocrater
longiflorus
Benth.
(Surahalala)
is
an
aromatic
perennial
plant
species
rich
in
secondary
metabolites.
It
exhibits
pharmaceutical
effects
due
to
essential
oils
and
terpenoid
compounds.
This
study
investigated
the
of
different
NaCl
concentrations
(0,
50,
100
mM),
as
salt
stress,
melatonin
levels
5,
15,
25
µM)
on
physiological
phyto-biochemical
traits
H.
under
in
vitro
cultivation.
The
results
showed
that
growth
parameters,
i.e.
fresh
weight,
shoot
length,
chlorophyll,
carotenoids,
relative
water
content,
were
maximized
by
applying
5
µM
melatonin.
High
salinity
(50
mM)
caused
a
significant
increase
malondialdehyde
hydrogen
peroxide
levels,
while
treatment
reduced
negative
oxidative
damage
longiflorus.
Total
phenol
phenylalanine
ammonia-lyase
enzyme
activity,
antioxidant
activity
increased
upon
treatments.
Under
stress
conditions,
certain
terpenes
(i.e.,
d-limonene,
α-thujene,
γ-terpinene)
increased,
others,
like
α-pinene,
copaene,
terpinen-4-ol,
decreased.
Notably,
application
at
higher
(5
resulted
elevated
oil
contents,
including
eremophila
ketone,
copaene.
However,
15
mM
combined
achieved
highest
γ-terpinene.
Our
findings
highlighted
melatonin’s
potential
modulate
improve
biosynthesis
terpenoids
longiflorus,
subjected
research
suggests
promising
strategies
active
phytochemicals
enhance
its
pharmacological
benefits
development
natural
products.
