Genome-Wide Identification of FCS-Like Zinc Finger (FLZ) Family Genes in Three Brassica Plant Species and Functional Characterization of BolFLZs in Chinese Kale Under Abiotic Stresses DOI Open Access
Yuwan Zhao,

Shunquan Chen,

Qin Mao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12907 - 12907

Published: Nov. 30, 2024

FCS-like zinc finger (FLZ) proteins are plant-specific regulatory proteins, which contain a highly conserved FLZ domain, and they play critical roles in plant growth stress responses. Although the family has been systematically characterized certain plants, it remains underexplored Brassica species, vital sources of vegetables, edible oils, condiments for human consumption sensitive to various abiotic stresses. Following whole-genome triplication events (WGT) Brassica, elucidating how genes have expanded, differentiated, responded stresses is valuable uncovering genetic basis functionality these genes. In this study, we identified total 113 from three diploid species classified them into four groups on their amino acid sequences. Additionally, 109 collinear gene pairs across dispersed among different chromosomes, suggesting that duplication (WGD) significantly contributed expansion family. Subcellular localization revealed six representative BolFLZ located nucleus cytoplasm. Yeast two-hybrid assays 13 selected BolFLZs interact with BolSnRK1α1 BolSnRK1α2, confirming conservation SnRK1α-FLZ module species. Expression profile analysis differential expression patterns tissues. Notably, levels seven out fifteen analyzed changed following treatment stressors, indicating distinct physiological respond uniquely Together, our results provide comprehensive overview insights potential applications enhancing tolerance Chinese kale.

Language: Английский

Quantifying redox signalling regulatory transcriptional dynamics in Nardostachys jatamansi under abiotic stress response DOI
Shubham Joshi, Rohit Joshi

Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2025, Volume and Issue: 1869(6), P. 130788 - 130788

Published: March 5, 2025

Language: Английский

Citations

0
Identification and analysis of abiotic stress-responsive bHLH gene family in Brassica oleracea DOI
Muhammad Adnan Raza,

Qin Wenjun,

Song Jiang-Hua

et al.

Horticulture Environment and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Language: Английский

Citations

0
McWRKY43 Confers Cold Stress Tolerance in Michelia crassipes via Regulation of Flavonoid Biosynthesis DOI Open Access

Qiuxiu Yu,

Caixian Liu,

Zhenyu Sun

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9843 - 9843

Published: Sept. 12, 2024

WRKY transcription factor (TF) plays a crucial role in plant abiotic stress response, but it is rarely reported

Language: Английский

Citations

3
BoPRR9, a pseudo-response regulator protein from cabbage, plays a negative regulatory role in the response to cold stress DOI

Yunyun Xing,

Muhammad Adnan Raza,

Yuxi He

et al.

Environmental and Experimental Botany, Journal Year: 2024, Volume and Issue: 224, P. 105801 - 105801

Published: May 7, 2024

Language: Английский

Citations

0
Physiological, transcriptomic, and metabolomic analyses of the chilling stress response in two melon (Cucumis melo L.) genotypes DOI

Qiannan Diao,

Shoubo Tian,

Yanyan Cao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

Abstract Background Chilling stress is a key abiotic that severely restricts the growth and quality of melon (Cucumis melo L.). Few studies have investigated mechanism response to chilling in melon. Results We characterized physiological, transcriptomic, metabolomic using two genotypes with different sensitivity (“162” “13-5A”). “162” showed higher osmotic regulation ability antioxidant capacity withstand stress. Transcriptome analysis identified 4395 4957 differentially expressed genes (DEGs) “13-5A” under stress, respectively. Metabolome 2347 differential enriched metabolites (DEMs), which were divided into 11 classes. Integrated transcriptomic enrichment glutathione metabolism, arginine proline expression patterns genotypes. Under metabolism-related DEGs (6-phosphogluconate dehydrogenase, peroxidase, s-transferase) upregulated “162,” GSH conjugates (L-gamma-glutamyl-L-amino acid L-glutamate) accumulated. Additionally, upregulation encoding ornithine decarboxylase, aspartate aminotransferase, pyrroline-5-carboxylate reductase, spermidine synthase increased arginine, ornithine, proline. Furthermore, transcription factors MYB, ERF, MADS-box, bZIP significantly upregulated, suggesting their crucial role tolerance Conclusions These findings elucidate molecular provide insights for breeding chilling-tolerant

Language: Английский

Citations

0
Response mechanisms of Annual bluegrass (Poa annua) to cold, drought, combined stresses and recovery in morphology, photosynthesis, physiology and microstructure DOI

Juanxia Li,

Xiaoming Bai,

Fu Ran

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 217, P. 109238 - 109238

Published: Oct. 25, 2024

Language: Английский

Citations

0
Construction of Freezing Injury Grade Index for Nanfeng Tangerine Plants Based on Physiological and Biochemical Parameters DOI Creative Commons
Chao Xu,

Buchun Liu,

Yuting Wang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 3109 - 3109

Published: Nov. 4, 2024

Low-temperature freezing stress constitutes the most significant meteorological disaster during overwintering period in Nanfeng Tangerine (NT) production area, severely impacting normal growth and development of plants. Currently, accuracy warnings forecasts for NT orchards remains suboptimal, primarily due to absence quantitative indicators low-temperature stress. Therefore, this study employed plants as experimental subjects conducted controlled treatment experiments under varying intensities (0 °C, −2 −5 −7 −9 °C) durations (1 h, 4 7 h). Subsequently, physiological biochemical parameters were measured, including photosynthetic parameters, chlorophyll fluorescence reactive oxygen species, osmoregulatory substances, antioxidant enzyme activities The results demonstrated that adversely affected system plants, disrupted dynamic equilibrium system, compromised cellular stability. severity damage increased with decreasing temperature prolonged exposure. Chlorophyll (a/b) ratio (Chl (a/b)), maximum quantum yield photosystem II (Fv/Fm), soluble sugar, malondialdehyde (MDA) identified key assessing changes Utilizing these four a comprehensive score (CS) model was developed quantitatively evaluate status across gradients durations. grade index established. Specifically, Level 1 CS ≤ −0.50, 2 −0.5 < 0, 3 0 0.5, 0.5 CS. research provide valuable data agricultural departments carry out monitoring, early warning, prevention control.

Language: Английский

Citations

0
Physiological, transcriptomic, and metabolomic analyses of the chilling stress response in two melon (Cucumis melo L.) genotypes DOI Creative Commons

Qiannan Diao,

Shoubo Tian,

Yanyan Cao

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 14, 2024

Chilling stress is a key abiotic that severely restricts the growth and quality of melon (Cucumis melo L.). Few studies have investigated mechanism response to chilling in melon. We characterized physiological, transcriptomic, metabolomic using two genotypes with different sensitivity ("162" "13-5A"). "162" showed higher osmotic regulation ability antioxidant capacity withstand stress. Transcriptome analysis identified 4395 4957 differentially expressed genes (DEGs) "13-5A" under stress, respectively. Metabolome 615 489 differential enriched metabolites (DEMs) were condition, Integrated transcriptomic enrichment glutathione metabolism, arginine (Arg) proline (Pro) expression patterns genotypes. Under metabolism-related DEGs, 6-phosphogluconate dehydrogenase (G6PDH), peroxidase (GPX), s-transferase (GST) upregulated "162," GSH conjugates (L-gamma-glutamyl-L-amino acid L-glutamate) accumulated. Additionally, upregulation DEGs encoding ornithine decarboxylase, Pro dehydrogenase, aspartate aminotransferase, pyrroline-5-carboxylate reductase, spermidine synthase increased Arg, ornithine, Pro. Furthermore, transcription factors (TFs), MYB, ERF, MADS-box, bZIP significantly upregulated, suggesting their crucial role tolerance These findings elucidate molecular provide insights for breeding chilling-tolerant

Language: Английский

Citations

0
Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance DOI

Khushbu Kumari,

Suman Gusain, Rohit Joshi

et al.

Planta, Journal Year: 2024, Volume and Issue: 261(1)

Published: Nov. 23, 2024

Language: Английский

Citations

0
Genome-Wide Identification of FCS-Like Zinc Finger (FLZ) Family Genes in Three Brassica Plant Species and Functional Characterization of BolFLZs in Chinese Kale Under Abiotic Stresses DOI Open Access
Yuwan Zhao,

Shunquan Chen,

Qin Mao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12907 - 12907

Published: Nov. 30, 2024

FCS-like zinc finger (FLZ) proteins are plant-specific regulatory proteins, which contain a highly conserved FLZ domain, and they play critical roles in plant growth stress responses. Although the family has been systematically characterized certain plants, it remains underexplored Brassica species, vital sources of vegetables, edible oils, condiments for human consumption sensitive to various abiotic stresses. Following whole-genome triplication events (WGT) Brassica, elucidating how genes have expanded, differentiated, responded stresses is valuable uncovering genetic basis functionality these genes. In this study, we identified total 113 from three diploid species classified them into four groups on their amino acid sequences. Additionally, 109 collinear gene pairs across dispersed among different chromosomes, suggesting that duplication (WGD) significantly contributed expansion family. Subcellular localization revealed six representative BolFLZ located nucleus cytoplasm. Yeast two-hybrid assays 13 selected BolFLZs interact with BolSnRK1α1 BolSnRK1α2, confirming conservation SnRK1α-FLZ module species. Expression profile analysis differential expression patterns tissues. Notably, levels seven out fifteen analyzed changed following treatment stressors, indicating distinct physiological respond uniquely Together, our results provide comprehensive overview insights potential applications enhancing tolerance Chinese kale.

Language: Английский

Citations

0