To
evaluate
effects
of
tertiary
treated
wastewater
treatment
plant
effluent
(MWWE)
on
transcriptomic
responses
in
longnose
dace
(Rhinichthys
cataractae;
LND)
we
conducted
a
semi-controlled
study
experimental
raceways
(Advancing
Canadian
Water
Assets
facility)
imbedded
the
Pine
Creek
(Calgary,
AB).
LND
collected
from
reference
site
Bow
River
(REF)
were
caged
containing
either
5%
(PC)
or
water
(BR;
control)
over
28
d.
Liver
transcriptomes
analyzed
males
and
females
sampled
days
7,
14
BR
PC,
compared
to
REF
fish
day
0.
Concurrent
with
caging,
selected
environmental
substances
concern
analysed
PC.
Significantly
different
unigenes
(SDUs)
(vs
males)
within
both
PC
increased
time
fish.
Moreover,
SDUs
same
(i.e.,
BR,
PC)
showed
temporal
increase
as
well
Time
was
dominant
factor
affecting
SDUs,
whereas
sex
had
less
an
impact
transcriptome
profiling.
Gene
Set
Enrichment
Analysis
vs
revealed
genes
involved
growth,
metabolism
carbohydrates
lipids,
immune
system
7;
however,
by
28,
80-100%
transcripts
localized
enriched
biomarkers
associated
tissue
sexes.
Exposure
significant
female
liver
somatic
index
but
no
observed
other
phenotypic
health
indices
sex.
used
source
water,
analyses
trace
amounts
ESOCs.
Analyses
did
not
point
towards
definitive
response
patterns
that
could
be
field-based
ecotoxicogenomic
studies
impacts
well-treated
MWWE
suggested
compromised
adaptive
responses.
Reviews in Aquaculture,
Journal Year:
2022,
Volume and Issue:
15(2), P. 491 - 535
Published: Sept. 5, 2022
Disease
and
parasitism
cause
major
welfare,
environmental
economic
concerns
for
global
aquaculture.
In
this
review,
we
examine
the
status
potential
of
technologies
that
exploit
genetic
variation
in
host
resistance
to
tackle
problem.
We
argue
there
is
an
urgent
need
improve
understanding
mechanisms
involved,
leading
development
tools
can
be
applied
boost
reduce
disease
burden.
draw
on
two
pressing
problems
as
case
studies-sea
lice
infestations
salmonids
white
spot
syndrome
shrimp.
review
how
latest
capitalised
upon
determine
underlying
inter-
intra-species
pathogen/parasite
resistance,
derived
knowledge
could
using
selective
breeding,
gene
editing
and/or
with
targeted
feed
treatments
vaccines.
Gene
brings
novel
opportunities,
but
also
implementation
dissemination
challenges,
necessitates
new
protocols
integrate
technology
into
aquaculture
breeding
programmes.
There
ongoing
minimise
risks
agents
evolving
overcome
improvements
insights
from
epidemiological
evolutionary
models
pathogen
infestation
wild
cultured
populations
are
explored.
Ethical
issues
around
different
approaches
achieving
discussed.
Application
has
fundamental
affecting
provide
effective
pathways
lead
more
resistant
stocks,
transforming
Toxics,
Journal Year:
2025,
Volume and Issue:
13(4), P. 269 - 269
Published: April 1, 2025
Perfluoroctane
sulfonate
(PFOS)
is
an
emerging
pollutant
widely
existing
in
aquatic
environments
that
has
attracted
many
scholars’
attention.
Cherax
quadricarinatus
(C.
quadricarinatus)
are
crustaceans
live
freshwater
environments.
This
study
aimed
to
investigate
the
long-term
toxic
exposure
effect
of
PFOS
on
C.
quadricarinatus.
Three
environment
concentrations
(1
ng/L,
100
and
10
μg/L)
were
set
for
28
days
The
results
indicated
was
detected
serum,
muscle,
hepatopancreas
quadricarinatus,
order
accumulation
levels
as
follows:
>
serum
muscle.
Furthermore,
transcriptomics
showed
function
differentially
expressed
genes
(DEGs)
groups
related
biological
processes,
metabolism,
organic
system,
immune
response.
Kyoto
Encyclopedia
Genes
Genomes
enrichment
analysis
DEGs
significantly
enriched
lysosome
signaling
pathway,
retinol
binding,
fructose
mannose
glutathione
etc.,
pathway
most
significant,
which
key
effects
Oncology Reports,
Journal Year:
2019,
Volume and Issue:
unknown
Published: Oct. 17, 2019
Esophageal
squamous
cell
carcinoma
(ESCC)
is
a
prevalent
aggressive
malignant
tumor
with
poor
prognosis.
Investigations
into
the
molecular
changes
that
occur
as
result
of
disease,
well
identification
novel
biomarkers
for
its
diagnosis
and
prognosis,
are
urgently
required.
Long
non‑coding
RNAs
(lncRNAs)
have
been
reported
to
play
critical
role
in
progression.
The
present
study
performed
data
mining
analyses
ESCC
via
an
integrated
accumulated
datasets
differentially
expressed
lncRNAs
from
Gene
Expression
Omnibus
(GEO)
Cancer
Genome
Atlas
(TCGA)
databases.
identified
intersection
genes
(lncRNAs,
miRNAs
mRNAs)
tissues
between
GEO
TCGA
was
investigated.
Based
on
these
intersected
lncRNAs,
constructed
competitive
endogenous
RNA
(ceRNA)
network
ESCC.
A
total
81
were
identified;
67
included
ceRNA
network.
Functional
revealed
key
primarily
dominated
cellular
biological
processes.
then
analyzed
associations
expression
levels
clinicopathological
characteristics
patients,
their
survival
time
using
TCGA.
results
31
associated
grade,
tumor‑node‑metastasis
(TNM)
stage
lymphatic
metastasis
status
(P<0.05).
In
addition,
15
demonstrated
be
Finally,
5
selected
validation
30
patients
newly
diagnosed
reverse
transcription‑quantitative
PCR
(RT‑qPCR).
suggested
fold
trends
up‑
downregulation
GEO,
RT‑qPCR
consistent.
it
also
select
few
significantly
TNM
lymph
node
clinically
relevant
analysis
aforementioned
bioinformatics
similar,
hence
proving
used
credible.
Overall,
may
provide
further
insight
functional
through
integrative
datasets,
reveal
potential
diagnostic
prognostic
Evolutionary Bioinformatics,
Journal Year:
2019,
Volume and Issue:
15, P. 117693431989228 - 117693431989228
Published: Jan. 1, 2019
Aquatic
ecosystems
that
form
major
biodiversity
hotspots
are
critically
threatened
due
to
environmental
and
anthropogenic
stressors.
We
believe
that,
in
this
genomic
era,
computational
methods
can
be
applied
promote
aquatic
conservation
by
addressing
questions
related
the
evolutionary
history
of
organisms
at
molecular
level.
However,
huge
amounts
genomics
data
generated
only
discerned
through
use
bioinformatics.
Here,
we
examine
applications
next-generation
sequencing
technologies
bioinformatics
tools
study
evolution
animals
discuss
current
challenges
future
perspectives
using
toward
animal
efforts.