Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase DOI

Baotong Zhu,

Quanhui Ye, Yoonjoo Seo

et al.

Environmental Science & Technology Letters, Journal Year: 2022, Volume and Issue: 9(7), P. 650 - 657

Published: June 17, 2022

The extensive production and use of polyethylene terephthalate (PET) have generated an enormous amount plastic waste, which potentially threatens the environment humans. Enzyme biocatalysis is a promising green chemistry alternative, relative to conventional fossil-derived process, achieve waste treatment recycling. In this work, we created biocatalyst, BIND-PETase, by genetically engineering curli Escherichia coli cell with functional PETase enzyme for biocatalytic degradation PET plastics. BIND-PETase could degrade generate products at concentration level greater than 3000 μM under various reaction conditions. effects key parameters, including pH, temperature, substrate mass load, surfactant addition were characterized. was reusable remained stable no significant activity loss when stored both 4 °C room temperature 30 days (Student's t test, p > 0.05). Notably, enable microplastics in wastewater effluent matrix. Moreover, depolymerize highly crystalline postconsumer materials ambient conditions efficiency 9.1% 7 days. This study provides new horizon developing environmentally friendly approaches solve recycling challenge.

Language: Английский

Insights into polyester plastic biodegradation by carboxyl ester hydrolases DOI
Alisa Gricajeva, Ashok Kumar, Renata Gudiukaitė

et al.

Journal of Chemical Technology & Biotechnology, Journal Year: 2021, Volume and Issue: 97(2), P. 359 - 380

Published: April 1, 2021

Abstract Environmental pollution caused by polyesters has become a major ecological safety concern that needs to be managed urgently. One way resolve this problem is giving the spotlight current emerging research of microbial biocatalysts. During last two decades many researchers have reported ability break down and modify natural synthetic using different carboxyl ester hydrolases (lipases, esterases, cutinases, PETases, etc.) also called polyesterases, contribution these enzymes towards reduction plastic levels in future. Continuous search such lipolytic biocatalysts their improvement via protein engineering strategies results beneficial findings making use polyesterases biodegradation plastics increasingly more realistic. The present review provides comprehensive insight into structural properties enabling lipolytic‐type effectively catalyze cleavage linkages polyester plastics. Moreover, management extensively used as an innovative eco‐friendly solution presented report. Furthermore, for development effective suitable polyester‐degrading summarized well. © 2021 Society Chemical Industry (SCI).

Language: Английский

Citations

68
Biodegradation of poly(ethylene terephthalate): Mechanistic insights, advances, and future innovative strategies DOI
Anamika Kushwaha, Lalit Goswami, Mamata Singhvi

et al.

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 457, P. 141230 - 141230

Published: Dec. 28, 2022

Language: Английский

Citations

68
Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective DOI Open Access

Shilpa Shilpa,

Nitai Basak, Sumer Singh Meena

et al.

Frontiers of Environmental Science & Engineering, Journal Year: 2022, Volume and Issue: 16(12)

Published: July 15, 2022

Language: Английский

Citations

57
Current Advances in the Biodegradation and Bioconversion of Polyethylene Terephthalate DOI Creative Commons

Xinhua Qi,

Wenlong Yan,

Zhibei Cao

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 10(1), P. 39 - 39

Published: Dec. 26, 2021

Polyethylene terephthalate (PET) is a widely used plastic that polymerized by terephthalic acid (TPA) and ethylene glycol (EG). In recent years, PET biodegradation bioconversion have become important in solving environmental pollution. More more hydrolases been discovered modified, which mainly act on degrade the ester bond of PET. The monomers, TPA EG, can be further utilized microorganisms, entering tricarboxylic cycle (TCA cycle) or being converted into high value chemicals, finally realizing Based synthetic biology metabolic engineering strategies, this review summarizes current advances modified hydrolases, engineered microbial chassis degrading PET, pathways artificial consortia bioconversion. Artificial consortium provides novel ideas for other complex polymers. It helpful to realize one-step chemicals.

Language: Английский

Citations

56
Enzymatic Degradation of Polyethylene Terephthalate Plastics by Bacterial Curli Display PETase DOI

Baotong Zhu,

Quanhui Ye, Yoonjoo Seo

et al.

Environmental Science & Technology Letters, Journal Year: 2022, Volume and Issue: 9(7), P. 650 - 657

Published: June 17, 2022

The extensive production and use of polyethylene terephthalate (PET) have generated an enormous amount plastic waste, which potentially threatens the environment humans. Enzyme biocatalysis is a promising green chemistry alternative, relative to conventional fossil-derived process, achieve waste treatment recycling. In this work, we created biocatalyst, BIND-PETase, by genetically engineering curli Escherichia coli cell with functional PETase enzyme for biocatalytic degradation PET plastics. BIND-PETase could degrade generate products at concentration level greater than 3000 μM under various reaction conditions. effects key parameters, including pH, temperature, substrate mass load, surfactant addition were characterized. was reusable remained stable no significant activity loss when stored both 4 °C room temperature 30 days (Student's t test, p > 0.05). Notably, enable microplastics in wastewater effluent matrix. Moreover, depolymerize highly crystalline postconsumer materials ambient conditions efficiency 9.1% 7 days. This study provides new horizon developing environmentally friendly approaches solve recycling challenge.

Language: Английский

Citations

53