Elemental and spectral chemometric analyses of Octopus vulgaris beaks as reliable markers of capture location

Journal of Food Science, Journal Year: 2023, Volume and Issue: 88(4), P. 1349 - 1364

Published: Feb. 15, 2023

The high demand and economic relevance of cephalopods make them prone to food fraud, including related harvest location. Therefore, there is a growing need develop tools unequivocally confirm their capture Cephalopod beaks are nonedible, making this material ideal for traceability studies as it can also be removed without loss commodity value. Within context, common octopus (Octopus vulgaris) specimens were captured in five fishing areas along the Portuguese coast. Untargeted multi-elemental total X-ray fluorescence analysis revealed abundance Ca, Cl, K, Na, S, P, concomitant with keratin calcium phosphate nature material. We tested suite discrimination models on both elemental spectral data, where elements contributing most discriminate location typically associated diet (As), human-related pressures (Zn, Se, Mn), or geological features (P, Mn, Zn). Among six different chemometrics approaches used classify individuals according beaks' element concentration, classification trees attained accuracy 76.7%, whilst reducing number explanatory variables sample highlighting variable importance group discrimination. However, using further improved accuracy, highest 87.3% found partial least-squares discriminant analysis. Ultimately, analyses nonedible structures such provide an important, complementary, easily accessible means support seafood provenance traceability, integrating anthropogenic and/or gradients.

Language: Английский

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Evaluation of fate of organic matter in sediment through microcosm experiments

Marine Ecology, Journal Year: 2024, Volume and Issue: 45(4)

Published: May 7, 2024

Abstract Laboratory microcosm experiments help to understand the degradation pattern of different sources derived from organic matter (OM) at sediment–water interface and relate it field processes. Microcosm were carried out for 65 days assess materials such as diatoms, zooplankton, mangrove leaves using source‐specific fatty acid (FA) biomarkers. The viable bacterial population in sediment was low on day 1, but 3, increased substantially by two threefold all treatments. An increase resulted a decrease FAs specific during initial incubation phase (10 days), suggesting sources. diatoms showed faster when compared zooplankton mangrove‐specific FAs. influenced nature source material, unsaturation FAs, type. Sandy facilitated higher diatom zooplankton‐specific whereas silty‐clay sediment. Thus, community composition coupled with habitat characteristics, OM composition, grain size sediment, long‐chain ~1.11–2.5 times spiked plankton‐derived labile mixed sources, which otherwise took longer time pointing toward co‐metabolism (priming effect), could play significant role cycling terrestrial plant‐derived an estuarine ecosystem.

Language: Английский

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Potential of Asparagopsis armata as a Biopesticide for Weed Control under an Invasive Seaweed Circular-Economy Framework

Biology, Journal Year: 2021, Volume and Issue: 10(12), P. 1321 - 1321

Published: Dec. 13, 2021

Marine macroalgae have been increasingly targeted as a source of bioactive compounds to be used in several areas, such biopesticides. When harvesting invasive species, Asparagopsis armata, for this purpose, there is two-folded opportunity: acquiring these biomolecules from low-cost resource and controlling its spreading impacts. The secondary metabolites seaweed's exudate shown significantly impact the physiology species ecosystems where it invades, indicating possible biocidal potential. Considering present work, an A. armata cocktail was applied model weed Thellungiella halophila evaluate physiological mode action, addressing potential use natural biocide. greatly affected test plants' physiology, namely, their photochemical energy transduction pathway (impairing light-harvesting chemical production throughout chloroplast electron transport chain), carotenoid metabolism oxidative stress. These mechanisms action are similar ones triggered when using common pesticides, highlighting eco-friendly biopesticide.

Language: Английский

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LipidTOX: A fatty acid-based index efficient for ecotoxicological studies with marine model diatoms exposed to legacy and emerging contaminants

Ecological Indicators, Journal Year: 2022, Volume and Issue: 139, P. 108885 - 108885

Published: April 21, 2022

Contaminants, when present above certain thresholds, can induce physiological constraints to organisms, namely diatoms, a model group representative of marine phytoplankton, triggering feedback mechanisms, such as changes in cell's fatty acid profiles, that be used biomarkers towards xenobiotic exposure. Having this mind and considering the ecological relevance diatom profiles well their recognized potential contaminant exposure, work aims develop test accuracy an integrative multi-biomarker response index based on diatoms (using Phaeodactylum tricornutum diatom) exposed several emerging contaminants. In terms impacts at individual level, it was possible observe transversal different contaminants, reduction C14:0 C16:0 acids, with increasing concentration, observed, for example, under propranolol fluoxetine Enhancement C16:2n-7 C16:3n-4 concentrations complete disruption basal profile observed copper nanoparticles. These diverse intrinsically connected alterations depended type dose applied, highlighting need address these whole. The evaluation cells' acids using multivariate approach revealed high degree sensitivity biochemical traits disclose applied exogenous concentration used. were later incorporated into unifying numerical (LipidTOX) integrated biomarker approach. LipidTOX showed strong correlations both growth features assessed cultures, revealing very efficiency translating impairments imposed by each xenobiotics tested concentrations. proved efficient tool ecotoxicological assays evidenced reliability classifying exposure cells results benefits application easily communicated non-expert audiences stakeholders, policymakers environmental managers so future toxicological evaluations classical primary producers.

Language: Английский

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Fatty acid-based index development in estuarine organisms to pinpoint environmental contamination

Marine Pollution Bulletin, Journal Year: 2022, Volume and Issue: 180, P. 113805 - 113805

Published: June 2, 2022

Estuaries have long been preferred areas of human settlement, where multiple anthropogenic activities take place, which contributed to a significant decrease in environmental quality these ecosystems. Accordingly, monitoring and management relied on the development tools that summarize simplify complex information provide direct interpretation status. Here, fatty acid profiles three abundant estuarine species, namely Hediste diversicolor, Carcinus maenas Pomatoschistus microps, were used develop validate multimetric index, based Euclidean dissimilarities between sites, response contamination gradient large urban estuary. Spatial differences generally related unsaturated acids (mono- polyunsaturated, n-3 n-6 series) all albeit more pronounced P. microps. Multivariate models returned high classification accuracies for sampled varying from 73.3% invertebrate species 100.0% fish species. Results show applicability developed FA-based particularly due easy communication, managers public alike, but also highlight need prior validation suitability or sensitivity depict contamination.

Language: Английский

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Fluoxetine induces photochemistry-derived oxidative stress on Ulva lactuca

Frontiers in Environmental Science, Journal Year: 2022, Volume and Issue: 10

Published: Aug. 24, 2022

Emerging pollutants impose a high degree of stress on marine ecosystems, compromising valuable resources, the planet and human health. Pharmaceutical residues often reach their input is directly related to activities. Fluoxetine an antidepressant, one most prescribed selective serotonin reuptake inhibitors globally has been detected in aquatic ecosystems concentrations up 40 μg L −1 . The present study aims evaluate impact fluoxetine ecotoxicity photochemistry, energy metabolism enzyme activity Ulva lactuca exposed environmentally relevant (0.3, 0.6, 20, 40, 80 ). Exogenous exposure induced negative impacts U. namely photosystem II antennae grouping fluxes. These included increased oxidative elevated enzymatic ascorbate peroxidase glutathione reductase. Lipid content altered levels key fatty acids such as hexadecadienoic (C16:2) linoleic (C18:2) revealed strong correlations with tested. Multivariate analyses reinforced chlorophyll fluorescence-derived traits efficient biomarkers for future toxicology studies.

Language: Английский

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Influence of Gender and Age of Brown Seaweed (Fucus vesiculosus) on Biochemical Activities of Its Aqueous Extracts

Foods, Journal Year: 2021, Volume and Issue: 11(1), P. 39 - 39

Published: Dec. 24, 2021

Fucus vesiculosus L. is a common coastal brown seaweed associated with various benefits to human health due its phenolic content and nutrients used as food through different methods of consumption. This study aims evaluate the influence seaweed's gender growth stage on types biological activities well chemical constitution elements present. Akin preparation, aqueous extracts were prepared at 25 °C (salad) 100 (soup). Biological determined by measuring total phenol (TPC), antioxidant activity inhibition acetylcholinesterase (AChE). Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS/MS) was for compound identification, elemental analysis carried out using Total Reflection X-ray Fluorescence (TXRF). Older females males had higher TPC compared new ones °C. Antioxidant depended extraction temperature but youngest male AChE inhibitory older °C, it females. Primary metabolites phloroglucinol main compounds identified. Additionally, since this often harvested in estuarine systems high anthropogenic impacts, safety evaluated evaluation sample's metal content. The heavy metals detected are within limits established regulating entities, pointing safe source.

Language: Английский

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Toxicology assessment of deep-sea mining impacts on Gigantidas platifrons: A comparative in situ and laboratory metal exposure study

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 933, P. 173184 - 173184

Published: May 13, 2024

Language: Английский

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Unravelling the Portuguese Coastal and Transitional Waters’ Microbial Resistome as a Biomarker of Differential Anthropogenic Impact

Toxics, Journal Year: 2022, Volume and Issue: 10(10), P. 613 - 613

Published: Oct. 15, 2022

Portugal mainland and Atlantic archipelagos (Madeira Azores) provide a wide array of coastal ecosystems with varying typology degrees human pressure, which shape the microbial communities thriving in these habitats, leading to development resistance traits. The samples collected on Portuguese northeast coast waters show an unequivocal prevalence Bacteria over Archaea high Proteobacteria, Cyanobacteria, Bacteroidetes Actinobacteria. Several taxa, such as Vibrio genus, showed significant correlations anthropogenic pollution. These pressures, along differences species diversity among surveyed sites, lead observed presence resistance-related sequences’ abundance (set all metal antibiotic resistant genes their precursors pathogenic non-pathogenic bacteria). Gene ontology terms resistance, redox regulation oxidative stress response were prevalent. A higher number found between sequences pollution, inorganic pressures density nearby population centres when compared taxa at different phylogenetic levels same environmental This points towards predominance conditions sequence rather than abundance. Our data suggest that whole resistome profile can more relevant or integrative answers disturbance environment, either grouped gene groups, appearing promising tool for impact assessment studies which, due ubiquity across microbes, be independently present samples.

Language: Английский

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