Conservation Genetics Resources, Journal Year: 2025, Volume and Issue: unknown
Published: May 5, 2025
Language: Английский
Environmental DNA, Journal Year: 2025, Volume and Issue: 7(1)
Published: Jan. 1, 2025
ABSTRACT Environmental DNA (eDNA) is an increasingly popular, sensitive, and cost‐efficient method for studying biodiversity detecting species. This noninvasive approach involves collecting environmental samples that contain genetic material shed by organisms into their surroundings. Due to the method's sensitivity, robust decontamination strategies are crucial, with sodium hypochlorite, commonly known as bleach, frequently employed. Despite its widespread use, there no consensus on most effective bleach concentration, leading inconsistencies in how chemical used research. study aimed determine minimum concentration of needed decontamination. Genomic signal crayfish was treated various concentrations ranging from 0.01% 5% (w/w). Results were observed using Qubit High Sensitivity reagents, quantitative PCR, agarose gel electrophoresis, Agilent TapeStation. Our results indicate a 0.5% (w/w) sufficient prevent detection genomic techniques tested. These provide important insights use eDNA Establishing standard protocols will help reduce enhance reliability studies.
Language: Английский
Citations
0
Diversity, Journal Year: 2025, Volume and Issue: 17(2), P. 84 - 84
Published: Jan. 23, 2025
Barrow Island, off the coast of north-west Western Australia, supports a rich subterranean amphipod fauna amid major resource development. Previous biological surveys for purpose species documentation and environmental impact assessment have helped to uncover once overlooked genus from family Eriopisidae. Here, we describe this new one species, Aenigmata megabranchia Stringer King gen. et sp. nov., using combination molecular morphological data, present key Australian eriopisid genera. The represents distinct, genetically divergent lineage that can be distinguished all other genera by following characters: shape setation mouthparts, enlarged coxal gills, straight posterior margin first coxa, an almost entirely cleft telson. This research enhances our knowledge Eriopisidae, emphasises importance Island as location fauna, will assist in future recognition conservation.
Language: Английский
Citations
0
Environmental DNA, Journal Year: 2025, Volume and Issue: 7(2)
Published: March 1, 2025
ABSTRACT Recent efforts have focused on reconstructing the historical abundance of unfossilized organisms using environmental DNA preserved in sediments (sedDNA). This information is crucial for understanding long‐term changes ecosystems. However, because sedDNA prone to degradation, its quantification may not always provide accurate estimates past abundances. To address this issue, we developed a novel method correct degradation by incorporating plankton remains and applied it estimate marimo—large spherical colonies green alga Aegagropila brownii (formerly A. linnaei )—in Lake Akan, Japan, which only known habitat large marimo. We first quantified marimo lake dating back over 200 years. then used our new their from sedDNA. Analyses revealed that were historically 10–100 times more abundant than they are today, but declined early 20th century when influxes muddy water level fluctuations occurred due deforestation operation hydroelectric power plant. These findings align with eyewitness accounts, indicating that, corrected fossilized remains, can be powerful tool organisms.
Language: Английский
Citations
0
Current Opinion in Environmental Science & Health, Journal Year: 2025, Volume and Issue: unknown, P. 100622 - 100622
Published: April 1, 2025
Language: Английский
Citations
0
Conservation Genetics Resources, Journal Year: 2025, Volume and Issue: unknown
Published: May 5, 2025
Language: Английский
Citations
0