Precision and Accuracy Limits of Wastewater-Based Epidemiology—Lessons Learned from SARS-CoV-2: A Scoping Review

Water, Journal Year: 2024, Volume and Issue: 16(9), P. 1220 - 1220

Published: April 25, 2024

Background: Wastewater-based epidemiology (WBE) has become crucial for early microbial outbreak detection and public health surveillance globally, underscored by the COVID-19 pandemic. However, despite advancements in sampling analyses, interpreting results estimating infection rates pose challenges. Enhancements sewer system engineering, understanding wastewater environment, addressing impact of environment on accuracy are needed. Objective: This scoping review aims to identify engineering knowledge gaps WBE guide future study designs. Design: Research “wastewater-based epidemiology” involving “engineering”, published between 2015 2023, was extracted from Scopus database. Results: examines elements influencing WBE’s precision reliability, especially identifying measuring SARS-CoV-2 RNA. It identifies significant effects analytical practices, wastewater’s composition performance. Conclusions: calls further investigation into economical evaluation methods these factors enhance data normalization interpretation, utilizing existing treatment plant used control, which could be a cost-effective approach over more expensive population biomarkers. approach, aside SARS-CoV-2, holds potential application broader number types diseases, as well consumption habits.

Language: Английский

---

Identification of environmental and methodological factors driving variability of Pepper Mild Mottle Virus (PMMoV) across three wastewater treatment plants in the City of Toronto

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 932, P. 172917 - 172917

Published: May 1, 2024

PMMoV has been widely used to normalize the concentration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA, influenza, and syncytial virus (RSV) account for variations in fecal content wastewater. is also as an internal RNA recovery control wastewater-based epidemiology (WBE) tests. While potentially useful interpretation WBE data, previous studies have suggested that can be affected by various physico-chemical characteristics There possibility laboratory methods, particularly variability centrifugation steps remove supernatant from pellets cause variability. The goal this study improve our understanding main drivers assessing relationship between concentration, wastewater, methodological approach concentrating wastewater samples. We analyzed 24-hour composite samples collected influent stream three treatment plants (WWTPs) located City Toronto, Ontario, Canada. Samples were 3 5 times per week starting beginning March 2021 mid-July 2023. flow rate was partition data into wet dry weather conditions. Physico-chemical (e.g., total suspended solids (TSS), biological oxygen demand (BOD), alkalinity, electrical conductivity (EC), ammonia (NH

Language: Английский

---

Comparison of Electronegative Filtration to Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(11), P. 2004 - 2013

Published: May 17, 2022

Methods of wastewater concentration (electronegative filtration (ENF) versus magnetic bead-based (MBC)) were compared for the analysis severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), beta-2 microglobulin, and human coronavirus OC43. Using ENF as method, two quantitative polymerase chain reaction (qPCR) analytical methods also compared: volcano second generation (V2G)-qPCR reverse transcriptase (RT)-qPCR measuring three different targets virus responsible COVID-19 illness (N1, modified N3, ORF1ab). Correlations between strong statistically significant SARS-CoV-2 (r = 0.77, p < 0.001) B2M 0.001). Comparison qPCR indicate that, on average, each method provided equivalent results with average ratios 0.96, 1.02 N3 to N1, ORF1ab, N1 ORF1ab supported by (p correlation coefficients 0.67 V2G (N3) RT (N1), r 0.74 (ORF1ab), 0.81 (N1) (ORF1ab)). Overall suggest that provide results, although variability is observed individual measurements. Given equivalency additional advantages disadvantages, described in discussion, are be considered when choosing an appropriate method.

Language: Английский

---

Wastewater surveillance uncovers regional diversity and dynamics of SARS-CoV-2 variants across nine states in the USA

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 877, P. 162862 - 162862

Published: March 16, 2023

Wastewater-based epidemiology (WBE) is a non-invasive and cost-effective approach for monitoring the spread of pathogen within community. WBE has been adopted as one methods to monitor population dynamics SARS-CoV-2 virus, but significant challenges remain in bioinformatic analysis WBE-derived data. Here, we have developed new distance metric, CoVdist, an associated tool that facilitates application ordination data identification viral changes based on nucleotide variants. We applied these approaches large-scale dataset from 18 cities nine states USA using wastewater collected July 2021 June 2022. found trends shift between Delta Omicron lineages were largely consistent with what was seen clinical data, offered added benefit revealing differences at state, city, even neighborhood scales. also able observe early variants concern presence recombinant during transitions variants, both which are challenging analyze clinically-derived genomes. The outlined here will be beneficial future applications SARS-CoV-2, particularly becomes less prevalent. Additionally, generalizable, allowing them outbreaks.

Language: Английский

---

Time series modelling for wastewater-based epidemiology of COVID-19: A nationwide study in 40 wastewater treatment plants of Belgium, February 2021 to June 2022

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 899, P. 165603 - 165603

Published: July 19, 2023

Language: Английский

---