An Update on Detection Technologies for SARS-CoV-2 Variants of Concern

Viruses, Journal Year: 2022, Volume and Issue: 14(11), P. 2324 - 2324

Published: Oct. 22, 2022

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is responsible for the global epidemic of Disease 2019 (COVID-19), with a significant impact on economy and human safety. Reverse transcription-quantitative polymerase chain reaction (RT-PCR) gold standard detecting SARS-CoV-2, but because virus’s genome prone to mutations, effectiveness vaccines sensitivity detection methods are declining. Variants concern (VOCs) include Alpha, Beta, Gamma, Delta, Omicron, which able evade recognition by host immune mechanisms leading increased transmissibility, morbidity, mortality COVID-19. A range research has been reported techniques VOCs, beneficial prevent rapid spread epidemic, improve public health social measures, reduce harm However, meaningful translation this that reduces burden disease, delivers clear cohesive message guide daily clinical practice, remains preliminary. Herein, we summarize capabilities various nucleic acid protein-based developed VOCs in identifying differentiating current compare advantages disadvantages each method, providing basis strains their future variants adoption corresponding preventive control measures.

Language: Английский

---

A method for sampling the living wood microbiome

Methods in Ecology and Evolution, Journal Year: 2024, Volume and Issue: 15(6), P. 1084 - 1096

Published: April 22, 2024

Abstract Efforts to characterize microbial life across diverse environments have progressed tremendously, yet the microbiome of Earth's largest biomass reservoir—the wood living trees—has been largely unexplored. Current understanding tree is confined roots and leaves, with little attention given endophytic wood, even though emergent studies indicated this zone as a niche for unique taxa, consequence ecosystem health global biogeochemical cycles. The lack investigation derives partly from physical recalcitrance which presents challenges during sampling, homogenization, extraction nucleic acids. In response these issues, we present an optimized method processing use in analyses, sampling through downstream analyses. Using methane‐cycling taxa model endophytes, assess losses recovery our method, determine limit‐of‐detection approximately 500 cells per 100 mg (dry) wood. For all six species evaluated—which represented several hardwoods softwoods—PCR inhibition proved minimal, expect be applicable majority species. methods presented herein can facilitate future into ecology methane cycling.

Language: Английский

---

Multiplexed detection, partitioning, and persistence of wild type and vaccine strains of measles, mumps, and rubella viruses in wastewater

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Wastewater surveillance of vaccine-preventable diseases may provide early warning outbreaks and identify areas to target for immunization. To advance wastewater monitoring measles, mumps, rubella viruses, we developed validated a multiplexed RT-ddPCR assay the detection their RNA. Because measles-mumps-rubella (MMR) vaccine is an attenuated live virus vaccine, also that distinguishes between wild-type strains measles in it using sample collected from facility with active outbreak. We evaluated partitioning behavior viruses liquid solid fractions influent wastewater. found assaying fraction resulted more sensitive despite fact viral RNA was enriched due low solids content Finally, investigated stability samples spiked over 28 days at two different concentrations temperatures (4°C room temperature) observed limited decay. Our study supports feasibility population-level surveillance.

Language: Английский

---

RBD amplicon sequencing of wastewater reveals patterns of variant emergence and evolution

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 12, 2024

Abstract Rapid evolution of SARS-CoV-2 has resulted in the emergence numerous variants, posing significant challenges to public health surveillance. Clinical genome sequencing, while valuable, limitations capturing full epidemiological dynamics circulating variants general population. This study utilized receptor-binding domain (RBD) amplicon sequencing wastewater samples monitor community and El Paso, TX. Over 17 months, we identified 91 observed waves dominant transitioning from BA.2 BA.2.12.1, BA.4&5, BQ.1, XBB.1.5. Our findings demonstrated early detection identification unreported outbreaks, showing strong consistency with clinical data at local, state, national levels. Alpha diversity analyses revealed periodical variations, highest winter outbreak lag phases, likely due lower competition among before growth phase. The underscores importance low transmission periods for rapid mutation variant evolution. highlights effectiveness integrating RBD surveillance tracking viral evolution, understanding emergence, enhancing preparedness.

Language: Английский

---

Viruses in Wastewater—A Concern for Public Health and the Environment

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1430 - 1430

Published: July 14, 2024

Wastewater monitoring provides essential information about water quality and the degree of contamination. Monitoring these waters helps identify manage risks to public health, prevent spread disease, protect environment. Standardizing appropriate most accurate methods for isolation identification viruses in wastewater is necessary. This review aims present major classes wastewater, as well concentration, isolation, assess health implement corrective measures control viral infections. Last but not least, we propose evaluate current strategies treatment new alternative disinfection.

Language: Английский

---