Mitochondrial Protein-Coding Gene Expression in the Lizard Sphenomorphus incognitus (Squamata:Scincidae) Responding to Different Temperature Stresses

Animals, Journal Year: 2024, Volume and Issue: 14(11), P. 1671 - 1671

Published: June 3, 2024

In the context of global warming, frequency severe weather occurrences, such as unexpected cold spells and heat waves, will grow, well intensity these natural disasters. Lizards, a large group reptiles, are ectothermic. Their body temperatures predominantly regulated by their environment temperature variations directly impact behavior physiological activities. Frequent periods waves can affect biochemistry physiology, often ability to maintain temperature. Mitochondria, center energy metabolism, crucial for maintaining temperature, regulating metabolic rate, preventing cellular oxidative damage. Here, we used RT-qPCR technology investigate expression patterns differences 13 mitochondrial PCGs in Sphenomorphus incognitus (Squamata:Scincidae), also known brown forest skink, under extreme stress at 4 °C, 8 34 38 °C 24 h, compared control 25 °C. southern China, lizards, is close lethal, induces hibernation, while 34/38 considered hot environmentally realistic. Results showed that low transcript levels ATP8, ND1, ND4, COI, ND4L significantly decreased, values 0.52 ± 0.08, 0.65 0.04, 0.68 0.10, 0.28 0.02, 0.35 respectively, with controls. By contrast, COIII exhibited significant increase, mean value 1.86 0.21. However, exposure h did not lead an increase levels. Indeed, ATP6, ND3, ND4 were downregulated, 0.48 0.11, 0.07, 0.41 0.54 Exposure led COII, COIII, ND5, CYTB, 3.3 0.24, 2.0 0.2, 2.70 1.06, 1.57 0,08, 1.47 0.13, 1.39 0.56, 0.12, over decrease (to 0.31 0.01) When exposed increased, ranging from 2.04 0.23 ND1 6.30 0.96 rise ND6. Under two different stress, genes S. vary, possibly associated strategies employed this species response high temperatures, allowing rapid compensatory adjustments electron transport chain proteins changes. Furthermore, underscores once again role function determining thermal plasticity reptiles.

Language: Английский

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Combined threats of climate change and contaminant exposure through the lens of bioenergetics

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(18), P. 5139 - 5168

Published: June 28, 2023

Organisms face energetic challenges of climate change in combination with suites natural and anthropogenic stressors. In particular, chemical contaminant exposure has neurotoxic, endocrine-disrupting, behavioral effects which may additively or interactively combine associated change. We used a literature review across animal taxa classes, but focused on Arctic endotherms contaminants important ecosystems, to demonstrate potential for interactive five bioenergetic domains: (1) energy supply, (2) demand, (3) storage, (4) allocation tradeoffs, (5) management strategies; involving four change-sensitive environmental stressors: changes resource availability, temperature, predation risk, parasitism. Identified examples included relatively equal numbers synergistic antagonistic interactions. Synergies are often suggested be particularly problematic, since they magnify biological effects. However, we emphasize that traits can equally reflect dampening beneficial responses result negative fitness. Our also highlights empirical demonstrations remain limited, especially endotherms. Elucidating the nature change-by-contaminant will build toward determining overall outcomes balance Progressing determine critical species, life stages, target areas transformative arise aid forecasting broad-scale under global scenarios.

Language: Английский

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Co-exposure of environmental contaminants with unfavorable temperature or humidity/moisture: Joint hazards and underlying mechanisms

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 264, P. 115432 - 115432

Published: Sept. 5, 2023

In the context of global climate change, organisms in their natural habitats usually suffer from unfavorable climatic conditions together with environmental pollution. Temperature and humidity (or moisture) are two central factors, while relationships toxicity contaminants not well understood. This review provides a synthesis existing knowledge on important interactions between contaminant temperature, soil moisture, air humidity. Both high temperature low moisture can extensively pose severe combined hazards organic pollutants, heavy metal ions, nanoparticles, or microplastics. There is more information effects animalia than other kingdoms. Prevalent mechanisms underlying joint include increased bioavailability bioaccumulation contaminants, modified biotransformation enhanced induction oxidative stress, accelerated energy consumption, interference cell membranes, depletion bodily fluids. However, humidity/moisture, particularly plants microorganisms, relatively vague need to be further revealed. work emphasizes that co-exposure chemical physical stressors results detrimental generally greater those caused by either stressor. It necessary take this into consideration ecological risk assessment both contamination change.

Language: Английский

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Consolidation of temperature-dependent toxicity and thermoregulatory behavior into risk assessments of insecticides under thermal scenarios: A prospective study on Eremias argus

Environment International, Journal Year: 2023, Volume and Issue: 172, P. 107742 - 107742

Published: Jan. 7, 2023

In this study, the temperature-dependent chemical toxicity of three insecticides and resulting thermoregulatory (TR) behavior lizard Eremias argus have been consolidated into current risk assessment framework. According to acute dermal assays, an increase ambient temperature from 15 °C 35 decreased beta-cyfluthrin (BC) but increased chlorpyrifos (CPF). The avermectin (AVM) did not show significant responses. Based on thermal preference trials, lizards changed their body via TR adaptively reduce under sub-lethal doses, which can be understood as a "self-rescue" attenuating lethal effects. However, quotient indicated that effectiveness is limited. Metabolomics analysis showed six different metabolites (i.e., creatine, glutamate, succinate, N-acetylaspartate, acetylcholine, lactate) contributed changes. Biochemical assays insecticide residue results demonstrated BC, CPF, AVM affected in aspects biotransformation, oxidative stress, neurometabolic interference. This work clarifies ecotoxicological impacts representative reptiles toxicological understanding relevance. knowledge may improve ecological predictions agrochemical applications context global climate change.

Language: Английский

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The combined effects of atrazine and warming on environmental adaptability in lizards (Eremias argus) from the perspective of a life-history traits trade-off: Gender differences in trade-off strategies may reverse mortality risk

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 879, P. 163078 - 163078

Published: March 25, 2023

Language: Английский

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Temperature-Dependent Bioaccumulation, Metabolism, and Hepatotoxicity of Flufiprole in Lizards (Eremias argus)

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(31), P. 11612 - 11625

Published: July 25, 2023

As a phenylpyrazole insecticide, flufiprole is an important substitute for fipronil in the agricultural field of China. However, its bioaccumulation and metabolism terrestrial organisms especially lizards living area have rarely been investigated. ectothermic animal, are also sensitive to temperature changes. Considering global warming, this study measured bioaccumulation, metabolism, hepatotoxicity Chinese native lizard (Eremias argus) under different stresses. Lizards exposed flufiprole-contaminated soil adsorbed through skin was preferred accumulate liver brain. The oxidation product sulfone main metabolite both human microsomes, which were mainly metabolized by CYP3A19 or CYP3A4. concentration increased with tissues. In addition, more serious oxidative damage shown higher as glutathione (GSH), malondialdehyde (MDA), 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels after exposure. Flufiprole exposure induced lesions, these lesions became higher-temperature groups. This provided new insights into risk assessment warming.

Language: Английский

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Brumating Male Lizards (Eremias Argus) Cleverly Respond to Insecticide Exposure and Warming Temperatures: Survival Rate and Physical Condition Cannot Coexist

Published: Jan. 1, 2024

Climate change and agricultural chemicals are two major threats to the reptile populations, which can interact with each other. Brumation is a huge challenge for reptiles, affecting their survival rate reproduction. In this study, effects of bifenthrin high temperature on brumation lizards were explored. Male Eremias argus (a native Chinese lizard) exposed environmental-related concentrations (0.5 mg·kg−1 5.0 mg·kg−1) different temperatures (5 oC 8 oC) 12 weeks during brumation. Behavior, physical parameters, hormones, physiological biochemical indicators measured study overwintering strategies under stresses. The results indicate that pesticide exposure affect mode lizards. At normal temperatures, increases oxidative stress in lizards, leading damage nitrogen cycling. Insufficient energy storage increased risk illness mean not wake up good state breeding. Brumating warm face exposure, taking tactful way winter. Lizards depth by regulating testosterone levels bodies. Frequently awakened consume excessive energy, poor condition. Deep brumating activate total antioxidant capacity resist stress. From long-term perspective, warming climate pesticides increase mortality brumation, conducive individual population sustainability.

Language: Английский

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Brumating Male Lizards (Eremias Argus) Cleverly Respond to Insecticide Exposure and Warming Temperatures: Survival Rate and Physical Condition Cannot Coexist

Published: Jan. 1, 2024

Language: Английский

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Negative effects on the adaptive strategies of the lizards (Eremias argus) under starvation after exposure to Glufosinate-ammonium

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 287, P. 110036 - 110036

Published: Sept. 7, 2024

Language: Английский

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Scrambled eggs—Negative impacts of heat stress and chemical exposures on ovarian function in swine

Molecular Reproduction and Development, Journal Year: 2023, Volume and Issue: 90(7), P. 503 - 516

Published: Jan. 18, 2023

Abstract Exposure to environmental toxicants and hyperthermia can hamper reproduction in female mammals including swine. Phenotypic manifestations include poor quality oocytes, endocrine disruption, infertility, lengthened time conceive, pregnancy loss, embryonic defects. The ovary has the capacity for toxicant biotransformation, regulated part by phosphatidylinositol‐3 kinase signaling pathway. impacts of exposure mycotoxins pesticides on swine potential an emerging chemical class concern, per‐ polyfluoroalkylated substances, porcine are reviewed. negative impairments heat stress (HS) reproductive outcomes also described cumulative effect exposures, such as HS, when present conjunction with a is considered.

Language: Английский

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