A review of the mechanisms that confer antibiotic resistance in pathotypes of E. coli

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: April 4, 2024

The dissemination of antibiotic resistance in Escherichia coli poses a significant threat to public health worldwide. This review provides comprehensive update on the diverse mechanisms employed by E. developing antibiotics. We primarily focus pathotypes (e.g., uropathogenic ) and investigate genetic determinants molecular pathways that confer resistance, shedding light both well-characterized recently discovered mechanisms. most prevalent mechanism continues be acquisition genes through horizontal gene transfer, facilitated mobile elements such as plasmids transposons. discuss role extended-spectrum β -lactamases (ESBLs) carbapenemases conferring -lactam antibiotics, which remain vital clinical practice. covers key resistant mechanisms, including: 1) Efflux pumps porin mutations mediate broad spectrum including fluoroquinolones aminoglycosides; 2) adaptive strategies , biofilm formation, persister cell activation stress response systems, withstand pressure; 3) regulatory systems coordinating providing insights into potential targets for therapeutic interventions. Understanding intricate network is crucial development effective combat this growing crisis. By clarifying these we aim pave way design innovative approaches implementation prudent stewardship practices preserve efficacy current antibiotics ensure sustainable future healthcare.

Language: Английский

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Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 27, 2024

Abstract Previous studies reported microplastics (MPs), antibiotics, and antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs). There is still a lack of research progress on the origin, fate, impact hazards MPs ARGs WWTPs. This paper fills gap this regard. In our search, we used “microplastics”, “antibiotic genes”, “wastewater plant” as topic terms Web Science, checking returned results for relevance by examining titles abstracts. study mainly explores following points: (1) origins fate MPs, antibiotics WWTPs; (2) mechanisms action sludge biochemical pools; (3) impacts WWTPs spread ARGs; (4) harm inflicted environment human body. Contaminants sewage such ARGs, antibiotic-resistant bacteria enter soil water. can travel through food chain thus reach humans, leading to increased illness, hospitalization, even mortality. will enhance understanding among they inflict

Language: Английский

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Unveiling the Secrets of Acinetobacter baumannii: Resistance, Current Treatments, and Future Innovations

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6814 - 6814

Published: June 21, 2024

represents a significant concern in nosocomial settings, particularly critically ill patients who are forced to remain hospital for extended periods. The challenge of managing and preventing this organism is further compounded by its increasing ability develop resistance due extraordinary genomic plasticity, response adverse environmental conditions. Its recognition as public health risk has provided impetus the identification new therapeutic approaches infection control strategies. Indeed, currently used antimicrobial agents gradually losing their efficacy, neutralized newer mechanisms bacterial resistance, especially carbapenem antibiotics. A deep understanding underlying molecular urgently needed shed light on properties that allow

Language: Английский

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Alternative therapies recently applied in controlling farmed fish diseases: mechanisms, challenges, and prospects

Aquaculture International, Journal Year: 2024, Volume and Issue: 32(7), P. 9017 - 9078

Published: July 17, 2024

Abstract The aquaculture industry is suffering from significant financial setbacks due to an increasing frequency of disease outbreaks, posing a threat the sector’s sustainability. Various bacterial, viral, parasitic, and fungal pathogens have led massive mortalities in farmed fish worldwide. Throughout years, management diseases has predominantly centered around utilization conventional antibiotics chemicals. Nevertheless, their indiscriminate use given rise serious implications, including increase resistant pathogens, disruptions metabolic processes fish, degradation aquatic environment, presence drug residues products, potential human health. effective bio-based immunoprophylaxis alternative therapies been developed overcome these impediments. Recent therapeutic approaches encompass range strategies, phytotherapeutics, nanotherapeutics, probiotics, prebiotics, synbiotics, phage therapy, vaccination, quorum quenching, antimicrobial peptides, biosurfactants, bacteriocins, stem cells, diagnostic-based therapy. Advancements biotechnology significantly enhanced efficacy therapies. However, additional research essential refine approaches. Critical concerns, such as efficacy, cost, risks, availability, adverse effects on ecosystem, need be addressed establish guidelines for sustainable application aquaculture. This review will aquaculturists’ awareness recent used farming, mechanisms, challenges, impacts while promoting sustainability commercial

Language: Английский

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Dynamic evolution of antibiotic resistance genes in plastisphere in the vertical profile of urban rivers

Water Research, Journal Year: 2023, Volume and Issue: 249, P. 120946 - 120946

Published: Nov. 29, 2023

Language: Английский

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Genomic Insights of Predominant International High-Risk Clone ST2 Acinetobacter baumannii Isolates in Saudi Arabia

Journal of Global Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Carbapenem-resistant Acinetobacter baumannii (CRAB), particularly the globally prevalent ST2 clone, poses significant threats in Gulf Cooperation Council region through extensive drug resistance, high virulence, and efficient gene transfer. This study investigates epidemiological genomic characteristics of CRAB Jazan region, using whole genome sequencing to understand its transmission genetic determinants. Sixty-five clinical A. isolates exhibiting phenotypic resistance carbapenems (meropenem, imipenem, ertapenem) were collected from various tertiary hospitals region. The presence carbapenemase genes among these was examined two sets multiplex polymerase chain reaction (PCR). Forty-eight confirmed as producers selected for WGS analysis. Among 65 isolates, adult ICU exhibited highest prevalence (76.9%). Multiplex PCR identified 48 (73.8%) producers, all harboring blaOXA-51-like genes. Specifically, blaOXA-23 detected 39 (60%), blaNDM 8 (12.3%), blaOXA-24 one isolate. These carbapenemase-producing also carried virulence-associated related adherence, biofilm formation, immune evasion, iron acquisition regulation, serum resistance. Whole-genome (WGS) revealed that (81.2%) carbapenemase-positive belonged ST2, followed by ST85 ST107, with KL152 locus most common capsule type. indicates a rise clone Arabian Gulf, Saudi Arabia, underscores need ongoing surveillance less clones such ST107 manage public health risks effectively.

Language: Английский

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Wastewater surveillance for antibiotics and resistance genes in a river catchment: Spatiotemporal variations and the main drivers

Water Research, Journal Year: 2023, Volume and Issue: 251, P. 121090 - 121090

Published: Dec. 29, 2023

Language: Английский

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Stimulating the biofilm formation of Bacillus populations to mitigate soil antibiotic resistome during insect fertilizer application

Environment International, Journal Year: 2024, Volume and Issue: 190, P. 108831 - 108831

Published: June 18, 2024

Antibiotic resistance in soil introduced by organic fertilizer application pose a globally recognized threat to human health. Insect may be promising alternative due its low antibiotic resistance. However, it is not yet clear how regulate microbes reduce agricultural application. In this study, we investigated and resistome under black soldier fly (BOF) pot field systems. Our study shows that BOF could stimulate ARB (antibiotic resistant − bacteria) suppressive Bacillaceae the microbiome resistome. The carbohydrate transport metabolism pathway of was strengthened, which accelerated synthesis polysaccharides form biofilm antagonistic ARB, thus reduced We further tested Bacillus spp. microcosm assay, resulted significant decrease presence ARGs together with higher abundance key formation gene (epsA). This knowledge might help development more efficient bio-fertilizers aimed at mitigating enhancing health, particular, requirements global "One Health".

Language: Английский

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The therapeutic effect of engineered phage, derived protein and enzymes against superbug bacteria

Biotechnology and Bioengineering, Journal Year: 2023, Volume and Issue: 121(1), P. 82 - 99

Published: Oct. 26, 2023

Defending against antibiotic-resistant infections is similar to fighting a war with limited ammunition. As the new century unfolded, antibiotic resistance became significant concern. In spite of fact that phage treatment has been used as an effective means for more than century, researchers have had overcome many challenges superbug bacteria by manipulating phages and producing engineered enzymes. New enzymes enhanced properties impact on ability fight infections, which considered window hope future. This review, therefore, illustrates not only caused but also are being developed solve these issues. Our study found phages, proteins, can be in treating destroying biofilm them. Combining compounds other antimicrobial substances increase their effectiveness bacteria. Therefore, substitute antibiotics or combination treat patients

Language: Английский

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Characterization of Increased Extracellular Vesicle-Mediated Tigecycline Resistance in Acinetobacter baumannii

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(4), P. 1251 - 1251

Published: April 15, 2023

Carbapenem-resistant Acinetobacter baumannii (CRAB) is the most detrimental pathogen that causes hospital-acquired infections. Tigecycline (TIG) currently used as a potent antibiotic for treating CRAB infections; however, its overuse substantially induces development of resistant isolates. Some molecular aspects resistance mechanisms AB to TIG have been reported, but they are expected be far more complicated and diverse than what has characterized thus far. In this study, we identified bacterial extracellular vesicles (EVs), which nano-sized lipid-bilayered spherical structures, mediators resistance. Using laboratory-made TIG-resistant (TIG-R AB), demonstrated TIG-R produced EVs control TIG-susceptible (TIG-S AB). Transfer analysis AB-derived treated with proteinase or DNase recipient TIG-S showed EV proteins major factors in transfer. Additional transfer spectrum EV-mediated was selectively transferred Escherichia coli, Salmonella typhimurium, Proteus mirabilis. However, action not observed Klebsiella pneumonia Staphylococcus aureus. Finally, likely induce antibiotics. Our data provide direct evidence cell-derived components high, selective occurrence neighboring cells.

Language: Английский

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