Bioactivity evaluation of Polygonum minus and identification of synergistic phytochemicals with 5-fluorouracil for colon cancer treatment

Microchemical Journal, Journal Year: 2025, Volume and Issue: 210, P. 112917 - 112917

Published: Jan. 30, 2025

Language: Английский

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Novel 2-(5-Arylthiophen-2-yl)-benzoazole Cyclometalated Iridium(III) dppz Complexes Exhibit Selective Phototoxicity in Cancer Cells by Lysosomal Damage and Oncosis

Journal of Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 67(1), P. 691 - 708

Published: Dec. 23, 2023

A second-generation series of biscyclometalated 2-(5-aryl-thienyl)-benzimidazole and -benzothiazole Ir(III) dppz complexes [Ir(C^N)2(dppz)]+, Ir1–Ir4, were rationally designed synthesized, where the aryl group attached to thienyl ring was p-CF3C6H4 or p-Me2NC6H4. These new assessed as photosensitizers explore structure–activity correlations for their potential use in biocompatible anticancer photodynamic therapy. When irradiated with blue light, exhibited high selective potency across several cancer cell lines predisposed therapy; benzothiazole derivatives (Ir1 Ir2) best performers, Ir2 being also activatable green red light. Notably, when irradiated, induced leakage lysosomal content into cytoplasm HeLa cells oncosis-like death. The capability Ir photoinduce death 3D spheroids has been demonstrated. investigated can catalytically photo-oxidate NADH photogenerate 1O2 and/or •OH cell-free media.

Language: Английский

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3D printed arrowroot starch-gellan scaffolds for wound healing applications

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130604 - 130604

Published: March 5, 2024

Language: Английский

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Application of organoid technology in the human health risk assessment of microplastics: A review of progresses and challenges

Environment International, Journal Year: 2024, Volume and Issue: 188, P. 108744 - 108744

Published: May 11, 2024

Microplastic (MP) pollution has become a global environmental issue, and increasing concern been raised about its impact on human health. Current studies the toxic effects mechanisms of MPs have mostly conducted in animal models or vitro cell cultures, which limitations regarding inter-species differences stimulation cellular functions. Organoid technology derived from pluripotent adult stem cells broader prospects for predicting potential health risks to humans. Herein, we reviewed current application advancements opportunities different organoids, including brain, retinal, intestinal, liver, lung assess MPs. techniques accurately simulate complex processes reflect phenotypes related diseases caused by such as liver fibrosis, neurodegeneration, impaired intestinal barrier cardiac hypertrophy. Future perspectives were also proposed technological innovation risk assessment using extending lifespan organoids chronic toxicity MPs, reconstructing multi-organ interactions explore their studying microbiome-gut-brain axis effect

Language: Английский

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Spatial Metabolomics and Lipidomics Reveal the Mechanisms of the Enhanced Growth of Breast Cancer Cell Spheroids Exposed to Triclosan

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(29), P. 10542 - 10553

Published: July 11, 2023

Triclosan (TCS), an antimicrobial compound, is known to have potential endocrine-disruptive properties, but the underlying toxic mechanisms at metabolic level are not well understood. Here, we applied metabolomics and lipidomics combined with mass spectrometry imaging (MSI) unveil of enhanced growth MCF-7 breast cancer cell spheroids (CCS) exposed TCS. To obtain a wide coverage metabolites lipids by using MSI, used techniques matrix-assisted laser desorption/ionization (MALDI) MALDI coupled laser-postionization. The results showed that TCS sulfate penetrated into entire area 0–3 h both localized in inner 6 h. After 24 h, portion two compounds was released from CCS. Omic data indicated exposure induced alterations via several pathways, including energy metabolism biosynthesis glycerophospholipids glycerolipids. Further MSI revealed enhancement supply peripheral increase storage might contribute CCS This study highlights importance integrating metabolite distributions profiles reveal novel TCS-triggered endocrine disrupting effects.

Language: Английский

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Biomimetic microfluidic chips for toxicity assessment of environmental pollutants

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170745 - 170745

Published: Feb. 8, 2024

Language: Английский

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Insight into the differential toxicity of PFOA and PFBA based on a 3D-cultured MDA-MB-231 cell model

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 465, P. 133499 - 133499

Published: Jan. 11, 2024

Language: Английский

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3-D models for studying microplastics effects on humans

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 417 - 441

Published: Jan. 1, 2025

Language: Английский

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Multi-cell lines culturing system utilizing microfluidic three-dimensional additive manufactured polymeric scaffolds with controlled pumping

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113193 - 113193

Published: March 1, 2025

Language: Английский

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Three-Dimensional Cultured Human Nasal Epithelial Cell Model for Testing Respiratory Toxicity and Neurotoxicity of Air Pollutants

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Accumulating evidence suggests a strong correlation between air pollution and neurological disorders; however, appropriate models methodologies are currently lacking. In this study, human nasal RPMI 2650 cell model based on air-liquid interface culture was discovered to possess olfactory epithelial cells. Two short-chain per- polyfluoroalkyl substances (PFAS), PFBA PFHxA, were used validate the performance of model. RNA sequencing initially revealed adverse effects two PFAS at environmentally relevant concentrations. Their key functions, including barrier protection, solute transport, neuronal activity, separately investigated. Both PFHxA disrupted membrane integrity increased cellular transport capacity, as indicated by upregulation ABC transporters. Additionally, they inhibited tight junction proteins, ZO-1, claudin-3, occludin, while increasing mucin expression mucus secretion. exhibited stronger in most assays uniquely induced significant NOTCH1 (p < 0.05), highlighting its potential hazards neurons. This study proposed novel vitro test with matched respiratory end points, which expected improve toxicological research risk assessment pollutants.

Language: Английский

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