Copper Toxicity in Animals: A Review

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Language: Английский

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Merestinib inhibits cuproptosis by targeting NRF2 to alleviate acute liver injury

Free Radical Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Effects of Dietary Copper Sources and Levels on Liver Copper Metabolism and the Expression of Transporters in Growing Pigs

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 526 - 526

Published: Feb. 12, 2025

Research on the effects of organic and inorganic Cu sources metabolic processes mechanisms in pigs is lacking. This study investigated different copper (Cu) levels hepatic metabolism transporter factors growing pigs. Sixty healthy piglets (initial body weight 14.00 ± 0.30 kg) were randomly divided into four groups with five replicates three each. Four diets (AM, AH, BM, BH) had [Cu sulphate (CuSO4): A amino acids (Cu-AA): B] [supplemented (120 mg/kg DM): M, supplemented (240 H]. The pre-feeding period was 7 days, followed by a 45-day feeding period. Slaughter sample collection carried out 46th day formal Significant differences considered at p < 0.05. final average daily gain (ADG) Cu-AA significantly higher than those CuSO4 groups. Serum increased increasing supplementation days 20 40. concentrations muscle, liver, liver subcellular organelles In groups, kidneys faeces. both lysosomes cytosol higher, activities cathepsin D (CTSD), β-glucosidase (BGL), acid phosphatase (ACP) cytoplasm higher. Comparisons between showed that mRNA protein 1 (CTR1), ATPase copper-transporting beta (ATP7B), ceruloplasmin (CP), antioxidant (ATOX1), metallothionein (MT) lower group group, best performance 120 Cu. mRNAs for alpha (ATP7A), cytochrome c oxidase chaperone 17 (COX17), superoxide dismutase (CCS) decreasing trend better deposition, enhances utilisation Cu, reduces excretion, promotes expression relevant enzymes transporters liver.

Language: Английский

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Pan-cancer analysis uncovered the prognostic and therapeutic value of disulfidptosis

npj Precision Oncology, Journal Year: 2025, Volume and Issue: 9(1)

Published: Feb. 24, 2025

Disulfidptosis, a newly discovered cell death mode distinct from other programmed in lung and kidney cancer cells, is defined as extensive disulfide bonds to actin cytoskeleton proteins, leading contraction cytoskeletal disruption death. New pattern discoveries often drive advances tumor research. Therefore, the present study attempted decipher manifestation importance of disulfidptosis pan-cancer. Combining Clinical specimen immunofluorescence staining, single-cell analyses, spatial transcriptome we demonstrated Multi-omics analysis has revealed that genomic variants DNA methylation DRGs can affect prognosis patients with The nomogram based on Score model could accurately predict PF-562271, EHT-1864, IPA-3 are potential therapeutic agents targeting disulfidptosis. Collectively, this deciphered for first time pan-cancer developed assist clinicians predicting guiding individualized treatment patients.

Language: Английский

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Effect of spermidine on cuproptosis in follicular granulosa cells

British Poultry Science, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 8

Published: March 12, 2025

1. A study was conducted to investigate the effect of spermidine on cuproptosis in granulosa cells goose ovarian follicles. Granulosa from F2-F5 grade follicles Sichuan white geese were isolated and cultured.2. Copper sulphate, chloroquine administered (GC). The GC activity, intracellular Cu2+ content, antioxidant enzyme expression levels genes proteins related autophagy determined.3. results showed that significantly decreased reactive oxygen species (ROS) levels, mitochondrial membrane potential Cu2+content (p < 0.05), increased cell activity 0.001). At same time, ferredoxin 1 (FDX1) gene 0.001), lipoylated-dihydrolipoamide S-acetyltransferase (Lip-DLAT) protein LC3 After treated with mixed for 8 h, copper p62 FDX1 0.01) 0.05).4. In summary, reduced ROS accumulation, improved activity. It alleviated sulphate-induced lipoylated aggregation by inducing autophagy. This suggested has a broad application prospect alleviating GC.

Language: Английский

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Novel insights into copper-induced Chinese mitten crab hepatopancreas mitochondrial toxicity: oxidative stress, apoptosis and BNIP3L-mediated mitophagy

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 107335 - 107335

Published: March 1, 2025

Language: Английский

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The Role of Cuproptosis in Hyperoxia-Induced Lung Injury and Its Potential for Treatment

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 4651 - 4664

Published: April 1, 2025

Oxygen supplementation is essential for patients with a multitude of diseases but can cause severe hyperoxia-induced lung injury (HLI), necessitating the identification therapeutic targets to improve clinical outcomes. Cuproptosis, novel copper-dependent form cell death characterized by proteotoxic stress resulting from lipoylated protein aggregation and loss iron-sulfur cluster proteins, distinct other forms death. However, role cuproptosis in HLI remains unclear. We established an model MLE-12 cells C57BL/6 mice investigate involvement toxicity. observed time-dependent increase cuproptosis-related gene Fdx1 under hyperoxia. Moreover, hyperoxia activated membrane-associated copper transporter SLC31A1 significantly elevated levels cells, as well serum tissue mice. Further analysis revealed that altered expression genes without affecting DLAT levels, increased lipoylated-DLAT levels. ELISA, CCK-8 assays, HE staining, wet-to-dry weight ratio, bronchoalveolar lavage fluid demonstrated treatment inhibitor TTM reduced pro-inflammatory cytokines (TNF-α IL-1β) alleviated both Our study identifies HLI, providing new insights into pathogenesis hyperoxic potential strategies.

Language: Английский

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Intestinal Cu(II)/(I) Redox State Transformation Causes Cu(I) Overflow and Toxicity of the Gut and Liver in Zebrafish

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

Copper (Cu) has long been a concern for human health. While previous studies have explored the toxic effects of Cu, no study is available on relationship between Cu redox state transformation and biotoxicity in higher organisms. In this study, we gut liver toxicity caused by overflow Cu(I) at low doses exposure. Here, first elucidated digestive metabolic systems as main target sites systematic epidemiological analysis. Then, ICP-MS analysis verified that were top two Cu-high-accumulated organs zebrafish exposed to 10 100 μg/L waterborne 72 h. In-situ Cu(II) imaging techniques demonstrated exogenous was converted gut. Furthermore, transcriptomic sequencing revealed high induced cell cycle arrest G phase. However, substantial accumulation disrupted metabolism energy source nutrients supply, leading hepatic toxicity. This provides new insights into mechanism based emphasizes health risks associated with exposure systems.

Language: Английский

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Machine learning-driven prediction model for cuproptosis-related genes in spinal cord injury: construction and experimental validation

Frontiers in Neurology, Journal Year: 2025, Volume and Issue: 16

Published: April 23, 2025

Introduction Spinal cord injury (SCI) severely affects the central nervous system. Copper homeostasis is closely related to mitochondrial regulation, and cuproptosis a novel form of cell death associated with metabolism. This study aimed explore relationship between SCI construct prediction models. Methods Gene expression data patient samples from GSE151371 dataset were analyzed. The differential correlation 13 cuproptosis-related genes (CRGs) non-SCI identified, ssGSEA algorithm was used for immunological infiltration analysis. Unsupervised clustering performed based on differentially expressed CRGs, followed by weighted gene co-expression network analysis (WGCNA) enrichment Three machine learning models (RF, LASSO, SVM) constructed screen candidate genes, Nomogram model verification. Animal experiments carried out an rat model, including behavioral scoring, histological staining, electron microscopic observation, qRT-PCR. Results Seven CRGs showed samples, there significant differences in immune levels. divided 38 into two clusters (Cluster C1 Cluster C2). WGCNA identified key modules clusters, involvement pathways such as Ribosome HIF-1 signaling pathway. Four (SLC31A1, DBT, DLST, LIAS) obtained models, SLC31A1 performing best (AUC = 0.958). confirmed decrease scores rats group, pathological changes tissue sections, model. Discussion revealed close association cuproptosis. Abnormal four function, energy metabolism, oxidative stress, response, which detrimental recovery neurological function SCI. However, this has some limitations, unidentified SRGs, small sample size. Future research requires more vitro vivo deeply regulatory mechanisms develop intervention methods.

Language: Английский

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Enhanced Effects of Polystyrene Nanoplastics and Imidacloprid on Organ Damage in Loaches(Misgurnus Anguillicaudatus): Disrupted Gene Expression Leads to Imbalance in Lipid Metabolism

Published: Jan. 1, 2025

Language: Английский

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