Cell Reports Methods,
Journal Year:
2024,
Volume and Issue:
4(5), P. 100764 - 100764
Published: May 1, 2024
Co-assembling
enzymes
with
nanoparticles
(NPs)
into
nanoclusters
allows
them
to
access
channeling,
a
highly
efficient
form
of
multienzyme
catalysis.
Using
pyruvate
kinase
(PykA)
and
lactate
dehydrogenase
(LDH)
convert
phosphoenolpyruvic
acid
lactic
semiconductor
quantum
dots
(QDs)
confirms
how
enzyme
cluster
formation
dictates
the
rate
coupled
catalytic
flux
(kflux)
across
series
differentially
sized/shaped
QDs
2D
nanoplatelets
(NPLs).
Enzyme
kinetics
were
used
demonstrate
that
by
mixing
different
NP
systems
clusters,
>10×
improvement
in
kflux
is
observed
relative
free
enzymes,
which
also
≥2×
greater
than
enhancement
on
individual
NPs.
Cluster
was
characterized
gel
electrophoresis
transmission
electron
microscopy
(TEM)
imaging.
The
generalizability
this
mixed-NP
approach
improving
confirmed
application
seven-enzyme
system.
This
represents
powerful
for
accessing
channeling
almost
any
choice
constituting
cascade.
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(9), P. 1107 - 1107
Published: Sept. 3, 2024
Arabinoside
and
derived
nucleoside
analogs,
a
family
of
exhibit
diverse
typically
biological
activities
are
widely
used
as
antibacterial,
antiviral,
anti-inflammatory,
antitumor,
other
drugs
in
clinical
preclinical
trials.
Although
with
long
rich
history
the
field
medicinal
chemistry,
biosynthesis
arabinoside
has
only
been
sporadically
designed
studied,
it
remains
challenge.
Here,
we
constructed
an
vitro
multi-enzymatic
cascade
for
arabinosides.
This
artificial
biosystem
was
systematically
optimized,
involving
exquisite
pathway
design,
NADP
