The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater DOI Creative Commons
Katherine R. Harrison, Delaney Snead,

Anna Kilts

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 21, 2023

ABSTRACT Virus concentrations measured in municipal wastewater help inform both the water treatment necessary to protect human health and wastewater-based epidemiology. Wastewater measurements are typically PCR-based, interpreting gene copy requires an understanding of form stability nucleic acids. Here, we study persistence model virus genomes wastewater, protective effects provided by capsids, relative decay rates genome infectious viruses. In benchtop batch experiments at 25 °C, extraviral (+)ssRNA dsDNA amplicons degraded 90% within 15-19 minutes 1.6-1.9 hours, respectively. When encapsidated, T 90 for MS2 increased 424× T4 52×. The were similar a range amplicon sizes. For our phages T4, acid signal untreated disappeared shortly after viruses lost infectivity. Combined, these results suggest that most viral copies part intact particles, independent assay sizes, naked inactivation rates. These findings will be valuable interpretation measurements.

Language: Английский

Revisiting the Potential Role of Urine in Wastewater Surveillance for COVID-19 and Beyond DOI Creative Commons
Aaron Bivins

Environmental Science & Technology Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

Language: Английский

Citations

0
Precision public health in schools enabled by wastewater surveillance: A case study of COVID-19 in an Upstate New York middle-high school campus during the 2021–2022 academic year DOI Creative Commons
Haley R. Kappus-Kron, Dana Chatila,

Ainsley Mabel MacLachlan

et al.

PLOS Global Public Health, Journal Year: 2024, Volume and Issue: 4(1), P. e0001803 - e0001803

Published: Jan. 10, 2024

Wastewater surveillance provides a cost-effective and non-invasive way to gain an understanding of infectious disease transmission including for COVID-19. We analyzed wastewater samples from one school site in Jefferson County, New York during the 2021–2022 year. tested SARS-CoV-2 RNA once weekly compared those results with clinical COVID-19 cases school. The amount correlated number incident cases, best correlation being day lead time between sample cases. sensitivity positive predictive value correctly identify any up 7 days after collection ranged 82–100% 59–78% respectively, depending upon sample. specificity negative when was without case 67–78% 70–80%, observed this study suggests that might occur within before is identified wastewater. However, should still be considered as potential means school-level trends enable precision public health approaches tailored epidemiologic situation individual

Language: Английский

Citations

3
Examining the Impact of Different Scales on COVID-19 Monitoring in Sewerage Catchment Areas: Insights from Latvia DOI Creative Commons
Brigita Dejus, Dita Gudrā, Sandis Dejus

et al.

Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100676 - 100676

Published: March 1, 2025

Language: Английский

Citations

0
The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater DOI Creative Commons
Katherine R. Harrison, Delaney Snead,

Anna Kilts

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(37), P. 13757 - 13766

Published: Sept. 1, 2023

Virus concentrations measured in municipal wastewater help inform both the water treatment necessary to protect human health and wastewater-based epidemiology. Wastewater measurements are typically PCR-based, interpreting gene copy requires an understanding of form stability nucleic acids. Here, we study persistence model virus genomes wastewater, protective effects provided by capsids, relative decay rates genome infectious viruses. In benchtop batch experiments influent at 25 °C, extraviral (+)ssRNA dsDNA amplicons degraded 90% within 15-19 min 1.6-1.9 h, respectively. When encapsidated,

Language: Английский

Citations

9
Wastewater surveillance of the most common circulating respiratory viruses in Athens: The impact of COVID-19 on their seasonality DOI

Anastasia Zafeiriadou,

Lazaros Kaltsis,

Marios Kostakis

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 900, P. 166136 - 166136

Published: Aug. 10, 2023

Language: Английский

Citations

7
Advances in wastewater analysis revealing the co-circulating viral trends of noroviruses and Omicron subvariants DOI Creative Commons
Teresa Kumblathan, Yanming Liu, Mary Crisol

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 920, P. 170887 - 170887

Published: Feb. 11, 2024

Co-presence of enveloped and non-enveloped viruses is common both in community circulation wastewater. Community surveillance infections requires robust methods enabling simultaneous quantification multiple Using SARS-CoV-2 Omicron subvariants Norovirus (NoV) as examples, this study reports a method that integrates electronegative membrane (EM) concentration, viral inactivation, RNA preservation (VIP) with efficient capture enrichment the on magnetic (Mag) beads, direct detection beads. This provides improved recoveries 80 ± 4 % for 72 5 (Murine NoV). Duplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays newly designed degenerate primer-probe sets offered high PCR efficiencies (90–91 %) targets NoV (GI GII) were able to detect few 15 copies per reaction. technique, combined multiplex duplex successfully quantified variants same 94 influent wastewater samples collected from two large systems between July 2022 June 2023. The results showed temporal changes revealing an inverse relationship their emergence. demonstrated importance analytical platform ability sensitively determine pathogens will advance applications complementary public health tool.

Language: Английский

Citations

1
Wastewater based measures of COVID-19 and associations with children's absenteeism at grade schools DOI
Helena M. Solo‐Gabriele,

Gabriela Guevara,

Naresh Kumar

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 959, P. 178217 - 178217

Published: Dec. 27, 2024

Language: Английский

Citations

0
The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater DOI Creative Commons
Katherine R. Harrison, Delaney Snead,

Anna Kilts

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 21, 2023

ABSTRACT Virus concentrations measured in municipal wastewater help inform both the water treatment necessary to protect human health and wastewater-based epidemiology. Wastewater measurements are typically PCR-based, interpreting gene copy requires an understanding of form stability nucleic acids. Here, we study persistence model virus genomes wastewater, protective effects provided by capsids, relative decay rates genome infectious viruses. In benchtop batch experiments at 25 °C, extraviral (+)ssRNA dsDNA amplicons degraded 90% within 15-19 minutes 1.6-1.9 hours, respectively. When encapsidated, T 90 for MS2 increased 424× T4 52×. The were similar a range amplicon sizes. For our phages T4, acid signal untreated disappeared shortly after viruses lost infectivity. Combined, these results suggest that most viral copies part intact particles, independent assay sizes, naked inactivation rates. These findings will be valuable interpretation measurements.

Language: Английский

Citations

1