Metagenomic insights into novel microbial lineages with distinct ecological functions in the Arctic glacier foreland ecosystems

Environmental Research, Journal Year: 2023, Volume and Issue: 241, P. 117726 - 117726

Published: Nov. 19, 2023

Language: Английский

Impact of artisanal refining activities on bacterial diversity in a Niger Delta fallow land

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 16, 2024

Abstract Hydrocarbon pollution is a major ecological problem facing oil-producing countries, especially in the Niger Delta region of Nigeria. In this study, site that had been previously polluted by artisanal refining activity was investigated using 16S rRNA Illumina high-throughput sequencing technology and bioinformatics tools. These were used to investigate bacterial diversity soil with varying degrees contamination, determined gas chromatography-flame ionization detector (GC-FID). Soil samples collected from heavily (HP), mildly (MP), unpolluted (control sample, CS) portion study site. DNA extracted Zymo Research (ZR) Fungi/Bacteria MiniPrep kit, followed PCR amplification agarose gel electrophoresis. The microbiome characterized based on V3 V4 hypervariable regions gene. QIIME (Quantitative Insights Into Microbial Ecology) 2 software analyse sequence data. final data set covered 20,640 demultiplexed high-quality reads total 160 filtered OTUs. Proteobacteria dominated HP CS, while Actinobacteria sample MP. Denitratisoma , Pseudorhodoplanes Spirilospora leading genera HP, MP respectively. Diversity analysis indicated CS [with 25.98 ppm petroleum hydrocarbon (TPH)] more diverse than (with 490,630 TPH) 5398 TPH). A functional prediction revealed six modules dataset, metabolism covering up 70%, 11 metabolic pathways. This demonstrates higher concentration adversely impacts microbial diversity, creating narrow hydrocarbon-degrading species, addition obvious land ecosystem degradation caused activities. Overall, business significantly driving services losses Delta, which calls for urgent intervention, focus bioremediation.

Language: Английский

Functional genomic analysis of nutrient cycling of plant-soil continuum in the mossy biocrust in the Tengger Desert

Rhizosphere, Journal Year: 2023, Volume and Issue: 28, P. 100806 - 100806

Published: Nov. 4, 2023

Language: Английский