High-efficient selection of aptamers by magnetic cross-linking precipitation and development of aptasensor for 1-aminohydantoin detection

LWT, Journal Year: 2024, Volume and Issue: 199, P. 116128 - 116128

Published: April 24, 2024

Accurate and rapid detection of nitrofurantoin metabolite 1-aminohydantoin (AHD), as one the tissue-bound toxic metabolites in foods, is crucial. In this study, a fluorescent aptasensor was constructed for AHD, using anti-AHD aptamers firstly obtained by high-efficiency magnetic crosslinking precipitation (MCP)-SELEX. The AHD-specific were optimized identified their binding affinity specificity fluorescence-based assays molecular dynamics simulations. truncated aptamer AHD50-1, which exhibited highest (Kd = 86 nM) an excellent specificity, applied to construction aptasensor. Under conditions, showed low limit (0.2 ng/mL) wide dynamic linear range (0.25–150 ng/mL). For five types samples (pork, beef, shrimp, fish honey), recoveries ranged from 89.4% 110.04%, with coefficients variation ranging 1.43% 14.46%. These results proposed corroborate very well method HPLC indicating that developed provides efficient promising sustainable strategy monitoring AHD animal derived food.

Language: Английский

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Construction of a New 2D Coordination Polymer: Degradation Antibiotic Nitrofurantoin and Magnetism

Applied Organometallic Chemistry, Journal Year: 2024, Volume and Issue: 38(12)

Published: Aug. 13, 2024

ABSTRACT The synthesis of a trinuclear Co(II)‐based coordination polymer (CP), [Co 3 (HL) 2 (H O) 6 ·2.2H O] n (1) , was achieved via solvothermal method involving CoCl ·6H O and 3‐(2,4‐dicarboxylphenyl)‐2,6‐dicarboxylpyridine L). photocatalytic efficiency the complex evaluated for degradation various antibiotics ornidazole (ODZ), nitrofurantoin (NFT), dinitroaniline (DNZ), tinidazole (TDZ) in wastewater. Notably, NFT exhibited remarkable 91.5% within 20 min under optimal conditions. catalytic performance influenced by amount photocatalyst, concentration, solution pH, with conditions identified at 6‐mg catalyst dose, 30 mg/L pH 7. Trapping experiments highlighted roles superoxide hydroxyl radicals as primary active species process. Furthermore, magnetic behavior is also discussed detail.

Language: Английский

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Co-interaction of nitrofuran antibiotics and the saponin-rich extract on gram-negative bacteria and colon epithelial cells

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(8)

Published: June 5, 2023

Large-scale use of nitrofurans is associated with a number risks related to growing resistance these compounds and the toxic effects following from their increasing presence in wastewater environment. The aim study was investigate an impact natural surfactant, saponins Sapindus mukorossi, on antimicrobial properties nitrofuran antibiotics. Measurements bacterial metabolic activity indicated synergistic bactericidal effect samples nitrofurantoin or furazolidone, which were added. Their addition led more than 50% greater reduction viable cells without saponins. On other hand, no against human colon epithelial cell observed. It found that exposure antibiotics surfactants caused membranes be dominated by branched fatty acids. Moreover, reduced hydrophobicity surface making them almost completely hydrophilic. results have confirmed high affinity Pseudomonas strains. beneficial action group also demonstrated. This result opens promising prospects for S. mukorossi as adjuvant reduce emission into

Language: Английский

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Validated Spectrophotometric Method for the Determination of Nitrofuratoin in Pure and in its Dosage Form

International Journal of Science and Research (IJSR), Journal Year: 2024, Volume and Issue: 13(5), P. 1551 - 1558

Published: May 5, 2024

A simple, précis, rapid sensitive and accurate spectrophotometric methods have been developed for the estimation of Nitrofuratoin UV in pure form its pharmaceutical formulations based on oxidative coupling reaction with 2, Bipyridine reagent at P H -4 which is extractable 520 nm.Beer's law obeyed concentration range 0.4 -2.4 ml (4 -24 µgml -1 ).The method was applied directly easily analysis formulations.RSD found to be 0.1473% recovery 99.31%.The completely validated proven rugged.The interferences ingredients excipients were not observed.The repeatability performance proved established by point internal hypothesis through studies.

Language: Английский

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