Mitigating the transfer risk of antibiotic resistance genes from fertilized soil to cherry radish during the application of insect fertilizer

Environment International, Journal Year: 2025, Volume and Issue: 199, P. 109510 - 109510

Published: May 1, 2025

The transfer of antibiotic resistance genes (ARGs) from fertilized soil to vegetables, particularly those consumed raw, causes significant public health risks through the food chain. Black soldier fly larvae can efficiently convert animal manure into organic fertilizer with reduced resistance. This study utilized metagenomic sequencing investigate fields treated control (COF), black (BOF), and no fertilizer, aim assessing ARGs cherry radish. results indicated that BOF significantly richness abundance in both radish compared COF, reducing 13 ARG subtypes a 27.6% decrease Moreover, positive correlation was observed between mobile genetic elements (MGEs) virulence factors (VFs) ARGs, treatment resulting relative reduction 32.8% 29.1%, respectively. complexity networks involving MGEs, VFs, microbial communities 54.2%, 32.3%, lower, respectively, than COF treatment. Further analysis metagenomic-assembled genomes (MAGs) revealed co-occurrence VFs radish, indicating presence potential pathogenic antibiotic-resistant bacteria (PARB). Notably, these PARB radishes decreased by 45.6% COF. These findings underscore efficacy insect mitigating highlighting significance sustainable agricultural practices managing environmental associated ARGs.

Language: Английский

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Gut microbiome of black soldier fly larvae for efficient use and purification of organic waste: An environmentally friendly development concept

The Innovation Life, Journal Year: 2025, Volume and Issue: unknown, P. 100134 - 100134

Published: Jan. 1, 2025

<p>The growing global population and increasing human demands have led to a rise in organic waste volumes. These wastes contain potential risk substances like pathogenic bacteria (PB), antibiotics (ABs), heavy metals (HMs), microplastics (MPs), which can threaten public health through direct contact or food chain transmission. Black soldier fly larvae (BSFL) emerged as promising solution for management crop-livestock integration. They efficiently reduce matter, produce high-quality proteins fats, generate green biofertilizers, mitigate harmful greenhouse gas emissions. However, the specific mechanisms by BSFL transform fertilizers, remain unclear, significantly limiting further development application of bioconversion technology. In recent years, numerous studies revealed that gut microbiota possess functions such promoting degradation substrate nutrients, regulating host nutrient synthesis metabolism, directly indirectly inhibiting proliferation PB, biodegrading cometabolizing ABs, mitigating mobility HMs, MPs. Therefore, exploring developing could be great significance optimizing efficiency transformation, effectively reducing waste, their wide applications biomedicine energy sectors. This review systematically summarizes composition, function, environmental impacts microbiota, focusing on roles transformation We believe in-depth exploration this field will advance integrated systems, is essential achieving humanity's sustainable goals.</p>

Language: Английский

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Review: Opportunities and challenges for the pork industry from circular livestock waste management systems

animal, Journal Year: 2025, Volume and Issue: unknown, P. 101547 - 101547

Published: May 1, 2025

Language: Английский

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Biosafety assessment of laying hens fed different treatments of black soldier flies (Hermetia illucens) under doxycycline stress

Poultry Science, Journal Year: 2024, Volume and Issue: 103(9), P. 103965 - 103965

Published: June 7, 2024

The black soldier fly (BSF, Hermetia illucens) is a resource insect that can utilize livestock and poultry feces. However, BSFs may also increase the risk of transmission antibiotic resistance genes (AGRs) are widespread in farm environments. Therefore, we aimed to evaluate biosecurity risks different BSF treatments laying chicken food chain using "chicken manure-BSF-laying hens" model. Our results indicated significantly affected residue, ARGs, MGEs, bacterial resistance, microbial community composition hens fed BSFs. These be effectively reduced through starvation treatment high-temperature grinding treatment. Comprehensive assessment analysis revealed combined with milling (Group H) had greatest effect.

Language: Английский

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Safety and transfer of veterinary drugs from substrate to black soldier fly larvae

animal, Journal Year: 2024, Volume and Issue: 18(7), P. 101214 - 101214

Published: June 13, 2024

There is an increasing interest in edible insects Europe for feed and food purposes. Quantitative information on the transfer of chemical hazards from substrates to larvae needed evaluate safety aspects. This evaluation especially when organic or residual streams such as manure will be applied substrate, contributing a circular system. study investigated veterinary drugs spiked substrate black soldier fly (Hermetia illucens). Veterinary that are commonly administered chicken, fattening pigs, cattle regularly detected were included: three different antibiotics (enrofloxacin, oxytetracycline, sulfamethoxazole), coccidiostats (narasin, salinomycin, toltrazuril) one antiparasitic drug (eprinomectin). The chemicals insect reach final concentrations 0.5 5 mg/kg drug, 50 coccidiostats. Black reared week frass was quantified using liquid chromatography coupled with tandem mass spectrometry. Only oxytetracycline eprinomectin reduced average weight and/or survival larvae. 19.2% 12% enrofloxacin, 9.5% narasin, 8.1% eprinomectin, 3.9% 4.2% toltrazuril, 0.2% sulfamethoxazole, relative substrate. Mass-balance calculations revealed seem metabolize drugs, indeed, metabolites toltrazuril frass. In conclusion, insect-rearing should evaluated presence residues ensure (and food) safety, well because possible effects growth.

Language: Английский

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Screening of oxytetracycline-degrading strains in the intestine of the black soldier fly larvae and their degradation characteristics

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 124929 - 124929

Published: Sept. 1, 2024

Language: Английский

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Evolution of microbiome composition, antibiotic resistance gene loads, and nitrification during the on-farm composting of the solid fraction of pig slurry using two bulking agents

Environmental Research, Journal Year: 2023, Volume and Issue: 245, P. 117944 - 117944

Published: Dec. 16, 2023

Language: Английский

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Co-digestion of chicken manure and sewage sludge in black soldier fly larvae bioconversion system: bacterial biodiversity and nutrients quality of residues for biofertilizer application

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(57), P. 119804 - 119813

Published: Nov. 6, 2023

Language: Английский

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Exploring the role of the microbiome of the H. illucens (black soldier fly) for microbial synergy in optimizing black soldier fly rearing and subsequent applications

Environmental Pollution, Journal Year: 2024, Volume and Issue: unknown, P. 125055 - 125055

Published: Oct. 1, 2024

Language: Английский

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Characteristics and biological mechanism of protein degradation by the black solider fly (Hermetia illucens L.) larvae gut strain Bacillus subtilis S4

Food Chemistry, Journal Year: 2024, Volume and Issue: 464, P. 141791 - 141791

Published: Nov. 5, 2024

Language: Английский

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