Discovery of a polyurethane-degrading enzyme from the gut bacterium of plastic-eating mealworm

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136159 - 136159

Published: Oct. 17, 2024

Language: Английский

Effective multi-stage biodegradation of commercial bulk polyurethane by Clonostachys and Purpureocillium spp

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 908, P. 168329 - 168329

Published: Nov. 4, 2023

Dealing with plastic waste in an environmentally friendly and effective manner has been a conundrum that much research attempted to address. An attractive approach is biodegradation by microorganisms; however microorganisms have shown such potential are generally only able degrade surface layers of polymer materials. Herein we describe the multi-stage degradation commercial bulk polyurethane two strains fungi isolated from landfill. Moreover, demonstrate mechanisms not hydrolysis alone as usually reported, but decarboxylation well. The data presented here suggest chain scission at urethane groups involves terminal functional release CO2. Two strains, Clonostachys sp. PB54 Purpureocillium PB57 were both decrease mass 40 % after 90 days. Spectroscopic analysis revealed breakage ester linkages also detected variations hydrogen bonding over time, indicating initial amorphous regions followed destabilization more ordered, crystalline layers. Subsequent Liquid Chromatography Mass Spectrometry (LCMS) demonstrated capable liberating monomer-equivalent molecules (4,4'-methylenedianiline) material. In this work, these be significantly degrading short period producing small organic CO2, such, good prospects for development large-scale processing.

Language: Английский

Contaminant-degrading bacteria are super carriers of antibiotic resistance genes in municipal landfills: A metagenomics-based study

Environment International, Journal Year: 2024, Volume and Issue: 195, P. 109239 - 109239

Published: Dec. 25, 2024

Municipal landfills are hotspot sources of antimicrobial resistance (AMR) and also important habitats contaminant-degrading bacteria. However, high diversity antibiotic genes (ARGs) in hinders assessing AMR risks the affected environment. More concerned, whether there is co-selection or enrichment antibiotic-resistant bacteria these extremely polluted environments far less understood. Here, we collected metagenomic datasets 32 raw leachate 45 solid waste samples 22 municipal China. The resistome, were explored, then compared with other environmental types. Results showed that resistome contained 1,403 ARG subtypes, total abundance over levels natural reaching human feces sewage. Therein, 49 subtypes listed as top priority ARGs for future surveillance based on criteria landfills, mobilizable present pathogens. By comparing to those contaminated river environments, elucidated an potentials landfills. Bacteria Pseudomonadaceae, Moraxellaceae, Xanthomonadaceae Enterobacteriaceae deserved most concerns since 72.2 % hosts classified them. Klebsiella pneumoniae, Acinetobacter nosocomialis Escherichia coli abundant multidrug-resistant pathogenic species (∼10.2 microbiomes), but they rarely carried contaminant-degradation genes. Notably, several bacterial genera belonging Pseudomonadaceae had antibiotic-resistant, pathogenic, than Overall, findings highlight selection pathogens, provide significant insights into

Language: Английский