Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
264, P. 115499 - 115499
Published: Sept. 18, 2023
This
study
aimed
to
investigate
the
sublethal
effects
of
thiacloprid
on
microRNA
(miRNA)
expression
in
honeybees
(Apis
mellifera
L.)
and
role
a
specific
miRNA,
ame-miR-283-5p,
resistance.
The
high-throughput
small
RNA-sequencing
was
used
analyze
global
miRNA
profile
changes
orally
exposed
concentrations
(20
mg/L
4
mg/L)
for
72
h.
Thiacloprid
at
20
had
no
observed
adverse
effects.
In
addition,
bees
were
fed
with
mimics
or
inhibitors
increase
decrease
ame-miR-283-5p
expression,
its
P450
gene
(CYP9Q2
CYP9Q3)
examined.
susceptibility
also
detected.
results
showed
that
treatment
induced
11
five
differentially
expressed
miRNAs
(DEMs),
respectively.
Bioinformatic
analysis
suggested
DEMs
are
mainly
involved
regulation
growth
development,
metabolism,
nerve
conduction,
behavior.
downregulated
by
both
concentrations,
which
validated
using
quantitative
real-time
reverse
transcription
PCR
analysis.
Enhancing
significantly
inhibited
CYP9Q2
mRNA
protein
increased
toxicity,
while
reducing
promoted
decreased
susceptibility.
Our
revealed
novel
insecticide
resistance
honeybees.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(1), P. 192 - 192
Published: Jan. 18, 2024
The
honey
bee
Apis
mellifera
is
exposed
to
a
variety
of
biotic
and
abiotic
stressors,
such
as
the
highly
prevalent
microsporidian
parasite
Nosema
(Vairimorpha)
ceranae
neonicotinoid
insecticides.
Both
can
affect
physiology
microbial
gut
communities,
eventually
reducing
its
lifespan.
They
also
have
combined
effect
on
insect’s
survival.
use
bacterial
probiotics
has
been
proposed
improve
health,
but
their
beneficial
remains
an
open
question.
In
present
study,
western
bees
were
experimentally
infected
with
N.
spores,
chronically
thiamethoxam,
and/or
supplied
daily
homofermentative
bacterium
Pediococcus
acidilactici
MA18/5M
thought
bees’
tolerance
parasite.
Deep
shotgun
metagenomic
sequencing
allowed
response
microbiota
be
investigated
taxonomic
resolution
at
species
level.
All
treatments
induced
significant
changes
in
communities.
infection
increased
abundance
Proteus
mirabilis,
Frischella
perrara,
Gilliamella
apicola
reduced
Bifidobacterium
asteroides,
Fructobacillus
fructosus,
Lactobacillus
spp.
Supplementation
P.
overturned
some
these
alterations,
bringing
back
altered
close
relative
found
controls.
Surprisingly,
exposure
thiamethoxam
restored
modulated
by
ceranae.
This
study
shows
that
stressors
may
antagonistic
impact
communities
protective
against
dysbiosis
Nanomaterials,
Journal Year:
2025,
Volume and Issue:
15(8), P. 570 - 570
Published: April 8, 2025
The
excessive
levels
of
neonicotinoid
insecticides,
particularly
thiacloprid
(THI),
in
the
environment
have
become
a
significant
threat
to
ecosystems.
This
study
investigates
catalytic
degradation
THI
using
pinewood
biochar
(PBC),
zero-valent
iron
(ZVI),
and
ZVI/PBC
composite,
with
particular
focus
on
reaction
activity
modulation
mediated
by
organic
acids
(humic
acid:
HA
oxalic
OA).
Reductive
dechlorination
dominated
as
observed
Cl-
release
kinetics.
Compared
(39.73%),
OA
(73.44%)
addition
markedly
increased
removal
efficiency
ZVI/PBC,
which
alone
has
lower
efficacy,
i.e.,
37.29%.
increase
rate
was
attributed
its
enhanced
electron
transfer
capacity.
As
confirmed
electrochemical
characterization,
promotes
between
catalysts
(ZVI,
PBC,
or
ZVI/PBC),
thereby
improving
THI.
XRD/XPS
analyses
elucidated
that
preferentially
converted
passivating
Fe2O3/Fe3O4
reactive
FeOOH
formed
electron-conductive
Fe-COO
bonds,
suppressing
oxide
layer
formation.
PBC
amplified
these
effects
through
ZVI
dispersion
shuttling,
reducing
aggregation-induced
loss.
These
findings
provide
mechanistic
framework
for
optimizing
ligand-engineered
composites,
offering
practical
strategies
enhance
pesticide
remediation
acid-rich
environmental
systems.
