First description of the microbial diversity in the Amarillo River (La Rioja, Argentina); a natural extreme environment where the whole microbial community paints the landscape yellow DOI Creative Commons
Cecilia Bernardelli,

Deborah Colman,

Edgardo Donati

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 29, 2023

Abstract Background. The Amarillo River is an acidic river located in the mining district of Famatina, La Rioja, Argentina, named after vast yellow-ochreous iron precipitates deposited all through its course. Even though area has been exploited for metals extraction, waters are naturally due to environmental factors beyond mineralogy, where microbial species have a crucial role. Iron-oxidizing bacteria identified, however comprehensive analysis entire community this extreme environment not conducted. In work we explore, first time using high-throughput sequencing, bacterial as well so long neglected fungal diversity and Cueva de Pérez terraces, considered prehistoric analogues current basin. Furthermore, attempt untangle ecological relations between fungi co-occurrence network analysis. Fe(II)-enrichment cultures at different temperature nutritional conditions, mimicking settings river, were also analysed better understand roles prokaryotes oxidation processes. Results. findings highlight presence diverse with marked uncultured Acidimicrobiia found other environments apparently part yet uncharacterised universal diversity. Acidophiles iron-oxidising species, despite being low abundances samples, constitute core community, showing significant involvement intraspecies interactions. Moreover, both samples enrichment exhibit wide fungi, highly connected according Conclusions. Through utilization 16S-amplicon Venn diagrams networks, shaded light into intricate connections communities River. occurrence certain acidophiles within ancient terraces indicates their enduring significance environment’s cycle flexibility system. These reinforce importance fundamental geological agents collaborative precipitation minerals paint landscape yellow.

Language: Английский

Bacterial, Archaeal, and Eukaryotic Life of Volcanic Cave Features from Lava Beds National Monument, California, USA DOI

Molly Devlin,

Jennifer Hathaway, Diana E. Northup

et al.

Geomicrobiology Journal, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: Jan. 10, 2025

Volcanic caves present a variety of surfaces that host unique life, but most microbiology studies these environments to date have focused on colorful and sometimes abundant biofilms, while largely ignoring ooze hard secondary mineral deposits. In this study, we conducted 16S/18S rRNA gene sequencing with bacterial, archaeal, eukaryotic domain-specific primer sets, focusing broad spectrum volcanic cave features in Lava Beds National Monument grouped into 'microbial-dominant' (biofilms ooze) 'mineral-dominant' (secondary deposits). While there are differences between individual mineral-dominant features, collectively, microbial communities associated distinct from those microbial-dominant based prokaryotic beta diversity metrics. Mineral-dominant not merely subset the owing their similar alpha scores ASVs. Ooze is all other categories due near absence otherwise Actinobacteriota abundance taxa roles ammonia nitrite oxidation. Differences were sampling year rather than feature type, suggesting eukaryotes more transient lifestyle prokaryotes. Overall, results provide evidence microbiologically more-often-studied biofilms.

Language: Английский

Citations

1
A Multisensory Analysis of the Moisture Course of the Cave of Altamira (Spain): Implications for Its Conservation DOI Creative Commons
Vicente Bayarri, Alfredo Prada, Francisco García

et al.

Remote Sensing, Journal Year: 2024, Volume and Issue: 16(1), P. 197 - 197

Published: Jan. 3, 2024

This paper addresses the conservation problems of cave Altamira, a UNESCO World Heritage Site in Santillana del Mar, Cantabria, Spain, due to effects moisture and water inside cave. The study focuses on description methods for estimating trajectory zones humidity from external environment its eventual dripping valuable paintings. To achieve this objective, several multisensor remote sensing techniques, both aerial terrestrial, such as 3D laser scanning, 2D ground penetrating radar, photogrammetry with unmanned vehicles, high-resolution terrestrial techniques are employed. These tools allow detailed spatial analysis highlights importance dolomitic layer how it influences preservation ceiling, which varies according position, whether is sealed calcium carbonate, actively dripping, or not dripping. In addition, crucial role central fracture areas direct infiltration process examined. research aids understanding conserving site. It offers novel approach water-induced deterioration rock art professionals researchers.

Language: Английский

Citations

6
The geomicrobiology of limestone, sulfuric acid speleogenetic, and volcanic caves: basic concepts and future perspectives DOI Creative Commons
Paolo Turrini, Alif Chebbi, Filippo Pasquale Riggio

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 20, 2024

Caves are ubiquitous subterranean voids, accounting for a still largely unexplored surface of the Earth underground. Due to absence sunlight and physical segregation, caves naturally colonized by microorganisms that have developed distinctive capabilities thrive under extreme conditions darkness oligotrophy. Here, microbiomes colonizing three frequently studied cave types, i.e., limestone, sulfuric acid speleogenetic (SAS), lava tubes among volcanic caves, comparatively been reviewed. Geological configurations, nutrient availability, energy flows in key ecological drivers shaping through photic, twilight, transient, deep zones. Chemoheterotrophic microbial communities, whose sustenance depends on nutrients supplied from outside, prevalent limestone while elevated inorganic chemical is available SAS enabling primary production chemolithoautotrophy. The 16S rRNA-based metataxonomic profiles were retrieved previous studies employing Illumina platform sequencing prokaryotic V3-V4 hypervariable region compare community structures different systems environmental samples. Limestone overlapping bacterial phyla, with prevalence Pseudomonadota Actinomycetota , whereas co-dominance Campylobacterota members characterizes caves. Most profiling data so far collected twilight transient zones, zones remain elusive, deserving further exploration. Integrative approaches future geomicrobiology suggested gain comprehensive insights into types This review also poses novel research questions unveiling metabolic genomic microorganisms, paving way their potential biotechnological applications.

Language: Английский

Citations

6
Microbiological exploration of the Cueva del Viento lava tube system in Tenerife, Canary Islands DOI Creative Commons
Sara Gutiérrez-Patricio, Jorge R. Osman, José Luis Gonzalez‐Pimentel

et al.

Environmental Microbiology Reports, Journal Year: 2024, Volume and Issue: 16(2)

Published: April 1, 2024

Abstract Cueva del Viento, located in the Canary Islands, Spain, is Earth's sixth‐longest lava tube, spanning 18,500 m, and was formed approximately 27,000 years ago. This complex volcanic cave system characterized by a unique geomorphology, featuring an intricate network of galleries. Despite its geological significance, geomicrobiology Viento remains largely unexplored. study employed combination culture‐dependent techniques metabarcoding data analysis to gain comprehensive understanding cave's microbial diversity. The 16S rRNA gene approach revealed that coloured mats (yellow, red white) coating walls are dominated phyla Actinomycetota , Pseudomonadota Acidobacteriota . Of particular interest high relative abundance genus Crossiella which involved urease‐mediated biomineralization processes, along with presence genera associated nitrogen cycling, such as Nitrospira Culture‐dependent provided insights into morphological characteristics isolated species their potential metabolic activities, particularly for strains Streptomyces spp., Paenarthrobacter sp. Pseudomonas spp. Our findings underscore ideal environment studying diversity isolation characterization novel bacterial biotechnological interest.

Language: Английский

Citations

6
Gypsum Cave Biofilm Communities are Strongly Influenced by Bat- And Arthropod-Related Fungi DOI Creative Commons
Valme Jurado, Tamara Martín-Pozas, Ángel Fernández‐Cortés

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: June 3, 2024

Abstract The Gypsum Karst of Sorbas, Almeria, southeast Spain, includes a few caves whose entrances are open and allow the entry roosting numerous bats. Caves characterized by their diversity gypsum speleothems, such as stalactites, coralloids, crusts, etc. Colored biofilms can be observed on walls most caves, among which Covadura C3 were studied. objective was to determine influence that bat mycobiomes may have fungal communities biofilms. results indicate fungi retrieved from white yellow in Cave ( Ascomycota , Mortierellomycota Basidiomycota ) showed wide diversity, depending location, highly influenced population, guano arthropods thrive guano, while more strongly soil- arthropod-related ), due absence

Language: Английский

Citations

4
Uncovering Microbial Diversity and Community Structure of Black Spots Residing in Tomb Mural Painting DOI Creative Commons
Qiang Li, He Zhang, Zeng Wang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 755 - 755

Published: March 26, 2025

Microbes colonizing cultural artifacts are a ubiquitous phenomenon which may occur during burial, post-excavation, and storage periods, thereby seriously affecting sustainable heritage conservation. In this study, high-throughput sequencing technology was applied to analyze the microbial community structure in ancient mural paintings surrounding air, as well identify most characteristic taxa causing black spot contamination. The results showed that members of genera Gliomastix Ochroconis were highly abundant black-spots-contaminated areas rarely detected air uncontaminated paintings. Air samples two tombs no significant difference Chao1 Shannon indices, whereas statistically differences observed compared those collected from spots. taxonomic diversity soil-covered exhibited similar structures at genus level. Moreover, when other tombs, spots differed not only composition but also assembly processes co-occurrence patterns, such much less network complexity area. Functional predictions uncover presence functional profiles involved nitrogen cycling, organic matter degradation, animal human pathogens, representing potential threat relics public health. These advance our understanding impacts archeological excavations on variation tomb

Language: Английский

Citations

0
Microclimate, airborne particles, and microbiological monitoring protocol for conservation of rock-art caves: The case of the world-heritage site La Garma cave (Spain) DOI
Tamara Martín-Pozas, Ángel Fernández‐Cortés, Soledad Cuezva

et al.

Journal of Environmental Management, Journal Year: 2023, Volume and Issue: 351, P. 119762 - 119762

Published: Dec. 10, 2023

Language: Английский

Citations

7
Looking for Microbial Biosignatures in All the Right Places: Clues for Identifying Extraterrestrial Life in Lava Tubes DOI Creative Commons
Joseph J. Medley, Jennifer Hathaway, M. Spilde

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(15), P. 6500 - 6500

Published: July 25, 2024

Lava caves are home to a stunning display of secondary mineral speleothems, such as moonmilk and coralloids, well highly visible microbial mats. These features contain diverse under-characterized groups bacteria. The role these bacteria in the formation speleothems is just beginning be investigated. lava Big Island Hawai`i El Malpais National Monument, New Mexico (USA), share many morphologically similar speleothems. This study focused on investigating overlap across wide range two geographically distant sites. Through scanning electron microscopy (SEM) 16S rRNA gene analysis, we found that Hawaiian have greater alpha diversity beta separated by cave speleothem type. Many Actinobacteriota were higher abundance caves, while contained more unclassified at genus species level. Discovering will assist identifying formations may good candidates for finding life extraterrestrial bodies.

Language: Английский

Citations

2
Contributions of DNA Sequencing Technologies to the Integrative Monitoring of Karstic Caves DOI Creative Commons
Zélia Bontemps, Yvan Moënne‐Loccoz, Mylène Hugoni

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(20), P. 9438 - 9438

Published: Oct. 16, 2024

Cave microbiota knowledge has greatly expanded in the past decades, driven by development of molecular techniques, which allow an in-depth characterization diversity and its metabolic potential. This review focuses on contribution DNA sequencing technologies to depict cave microbiome for three domains life (Bacteria, Archaea, Microeukaryotes), assessing their advantages limits. Cultural methods do not provide a representative view microbial but offer, subsequently, possibility genomically characterize strains isolated from caves. Next-generation permits exhaustive description biodiversity caves, using metabarcoding (for taxonomic assessment) or metagenomics functional assessments). It proved useful compare different rooms, substrata (water, soil, bedrock, etc.) within cave, effect disturbance Lascaux elsewhere. The integration next-generation with cultivation physico-chemical characterization, other complementary approaches is important understand global functioning caves key information guide conservation strategies.

Language: Английский

Citations

2
Bacterial communities forming yellow biofilms in different cave types share a common core DOI
Tamara Martín-Pozas, Valme Jurado, Ángel Fernández‐Cortés

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 956, P. 177263 - 177263

Published: Nov. 8, 2024

Language: Английский

Citations

2