Detection of enteroviruses related to hand foot and mouth disease in wastewater of Asian communities DOI
Sadhana Shrestha, Bikash Malla, Jatuwat Sangsanont

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 169375 - 169375

Published: Dec. 16, 2023

Language: Английский

Evaluation of plasmid pBI143 for its optimal concentration methods, seasonal impact, and potential as a normalization parameter in wastewater-based epidemiology DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 965, P. 178661 - 178661

Published: Feb. 1, 2025

Language: Английский

Citations

2
Estimation of Norovirus infections in Japan: An application of wastewater-based epidemiology for enteric disease assessment DOI
Sadhana Shrestha, Bikash Malla, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 169334 - 169334

Published: Dec. 14, 2023

Language: Английский

Citations

21
Optimization of the 5-plex digital PCR workflow for simultaneous monitoring of SARS-CoV-2 and other pathogenic viruses in wastewater DOI
Bikash Malla, Sadhana Shrestha, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 913, P. 169746 - 169746

Published: Dec. 28, 2023

Language: Английский

Citations

19
Beyond COVID-19: Wastewater-based epidemiology for multipathogen surveillance and normalization strategies DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Niva Sthapit

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174419 - 174419

Published: July 2, 2024

Wastewater-based epidemiology (WBE) is a critical tool for monitoring community health. Although much attention has focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative agent of disease 2019 (COVID-19), other pathogens also pose significant health risks. This study quantified the presence SARS-CoV-2, influenza A virus (Inf-A), and noroviruses genogroups I (NoV-GI) II (NoV-GII) in wastewater samples collected weekly (n = 170) from July 2023 to February 2024 five treatment plants (WWTPs) Yamanashi Prefecture, Japan, by quantitative PCR. Inf-A RNA exhibited localized prevalence with positive ratios 59 %–82 % different WWTPs, suggesting regional outbreaks within specific areas. NoV-GI (94 %, 160/170) NoV-GII (100 170/170) were highly prevalent, (6.1 ± 0.8 log10 copies/L) consistently exceeding (5.4 0.7 concentrations. SARS-CoV-2 was detected 100 samples, mean concentrations 5.3 0.5 copies/L WWTP E 5.8 0.4 each WWTPs. Seasonal variability evident, higher all pathogenic viruses during winter. Non-normalized normalized fecal indicator bacteria (Escherichia coli total coliforms), an (pepper mild mottle (PMMoV)), turbidity revealed associations reported cases. + GII showed strong correlations gastroenteritis cases, particularly when E. (Spearman's ρ 0.70–0.81) coliforms (ρ 0.70–0.81), respectively. For non-normalized correlation 0.61, decreasing 0.31 PMMoV, that PMMoV unsuitable. Turbidity normalization yielded suboptimal results. underscored importance selecting suitable parameters tailored accurate trend using WBE, demonstrating its utility beyond COVID-19 surveillance.

Language: Английский

Citations

5
Potential therapeutic substances for hand-foot-and-mouth disease in the interplay of enteroviruses and type I interferon DOI
Xinyu Wang, Ziyuan Wang,

Zhongtian Qi

et al.

International Journal of Antimicrobial Agents, Journal Year: 2025, Volume and Issue: unknown, P. 107464 - 107464

Published: Feb. 1, 2025

Language: Английский

Citations

0
Evaluation of the Enterovirus serotype monitoring approach for wastewater surveillance of hand foot and mouth disease using secondary epidemiological surveillance data DOI Creative Commons
Sadhana Shrestha, Bikash Malla, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 969, P. 178896 - 178896

Published: Feb. 26, 2025

Language: Английский

Citations

0
Optimization of a 6-plex Crystal Digital PCR® assay and its application to simultaneous surveillance of enteric and respiratory viruses in wastewater DOI Creative Commons
Bikash Malla, Sadhana Shrestha, Eiji Haramoto

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 970, P. 178939 - 178939

Published: March 1, 2025

Multiplex digital PCR (dPCR) approaches are commonly employed in wastewater-based epidemiology (WBE) studies. However, optimizing the dPCR workflow is a critical step to ensure its reliability and accuracy before application. In this study, 6-plex Crystal Digital PCR® (cdPCR) was optimized for simultaneous detection of six epidemiologically important pathogens, including three enteric viruses, noroviruses genogroups I II (NoV-GI GII) enteroviruses (EnV), respiratory severe acute syndrome coronavirus 2 (SARS-CoV-2), which causative agent disease 2019 (COVID-19), influenza A virus (InfA), syncytial B (RSVB), wastewater. Four cDNA input ratios (20 %-70 %) two extraction kits were evaluated optimization, with 30 % AllPrep PowerViral DNA/RNA Kit (Qiagen) exhibiting optimal performance. The cdPCR assay applied year-long wastewater surveillance study Japan (n = 52), revealing distinct trends prevalence viruses. NoV-GII detected 96 samples highest mean concentration (6.1 ± 0.6 log10 copies/L), while SARS-CoV-2 InfA 60 50 samples, respectively, reflected circulation these pathogens within community. Notably, RSVB less frequently (25 %), line fewer cases reported during period. concentrations EnV showed significant positive correlations hand foot mouth herpangina cases, respectively. no observed RSV COVID-19, possibly due testing RSVA more prevalent also cluster outbreaks. These findings demonstrated utility detecting provided insights into community trends, representing an advancement WBE.

Language: Английский

Citations

0
Defining alarm thresholds for the load of pathogenic viruses in wastewater for decision making: An application to three French cities DOI
Olivier Schlosser, Sophie Courtois,

Philippe Bryche

et al.

International Journal of Hygiene and Environmental Health, Journal Year: 2025, Volume and Issue: 266, P. 114563 - 114563

Published: March 15, 2025

Language: Английский

Citations

0
Detection of SARS-CoV-2 and Omicron variant RNA in wastewater samples from Manila, Philippines DOI
Jessamine Gail M. Inson, Bikash Malla, Divina Amalin

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170921 - 170921

Published: Feb. 11, 2024

Language: Английский

Citations

3
Prevalence of hepatitis A and E viruses in wastewater in Asian countries DOI Creative Commons

Sunayana Raya,

Sarmila Tandukar,

Hari Prasad Kattel

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175473 - 175473

Published: Aug. 12, 2024

Hepatitis A and E viruses (HAV HEV, respectively) remain a significant global health concern despite advancements in healthcare vaccination programs. Regular monitoring vaccine efficacy of HAV are still lacking different countries. This study aimed to investigate HEV prevalence developed, developing, least-developed Asian countries using wastewater as surveillance tool. total 232 untreated samples were collected from six treatment plants, sewage plant, or an open drainage [Nepal (n = 51), Indonesia 37), Thailand 30), Vietnam 27), the Philippines 17), Japan 70)] between April October 2022. Viruses concentrated by simple centrifugation polyethylene glycol precipitation method, followed viral RNA extraction reverse transcription-quantitative polymerase chain reaction. detected Nepal (51 % for 2 HEV), (3 both viruses), (1 24 HEV). Only was found 11 Indonesia, whereas only Philippines, with positive ratio 15 12 %, respectively. These results highlighted geographic variability prevalence, underscoring need localized public strategies address specific hepatitis challenges each country.

Language: Английский

Citations

3