Investigating the potential of X shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage X-chromosome meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Language: Английский

Toxicology of chemical biocides: Anticoagulant rodenticides – Beyond hemostasis disturbance DOI
Aleksandra Popov Aleksandrov, Dina Tucović, Jelena Kulaš

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 277, P. 109841 - 109841

Published: Jan. 17, 2024

Language: Английский

Citations

4
Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic DOI Creative Commons
Marcela Fraňková, Zuzana Starostová,

Radek Aulický

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 11, 2025

Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast studies on Western European house mice (Mus musculus domesticus), few resistance have focused Eastern (M. musculus), which a western distribution boundary Czech Republic. This study newly analysed VKORC1 gene M. m. field populations from farms grain stores identified nonsynonymous mutation Tyr139Phe. was common throughout Republic present 80.2% 86 individuals sampled. Additionally, all exhibited genotype with three synonymous mutations specific subspecies musculus. The functional (mortality–survival) response Tyr139Phe validated laboratory choice feeding test using bromadiolone-based bait, where resistant homozygous survived, while susceptible died, mean survival 6.9 days.

Language: Английский

Citations

0
More than garden plants: extending the conversation of urban gardens as an important refuge for Australian birds DOI Creative Commons
Rochelle Steven, David Newsome

Biodiversity and Conservation, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Inadequacies in public protected area networks dictates that private land will play an important role the conservation of biodiversity coming decades. Household gardens are a key example lands can serve as refuges for biodiversity, with birds popular flagship garden biodiversity. Discussion has focused heavily on species plant resident might select to attract their garden. In this paper, we describe additional and factors should form part broader conversation specific aim drawing attention at risk localised extinctions modern urban landscapes – adaptors. We present our commentary two themes: (a) mitigating threats environment (b) enhancing habitat value range bird species. provide synthesis research recent years explored its ability support birds, but importantly, extend by bringing together topics have been somewhat lacking discussion date. new approach conversation, paper brings no longer be considered isolation if make significant gains environments were most Australians now reside.

Language: Английский

Citations

0
Maximising Eradication Potential of Rat Gene Drives Using a Two‐Target Homing Rescue Strategy: Spatial Modelling of Empirical Data DOI Creative Commons
Ayşegül Birand, Luke Gierus, Thomas A. A. Prowse

et al.

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

ABSTRACT Gene drives are genetic elements with positively biased transmission and may be useful tools to suppress mammalian pests that threaten biodiversity worldwide. While gene progressing in mice, less is known about their potential for invasive rat control. A recent report has provided the first data on germline conversion rats, demonstrating modest homing rates (up 67%) can achieved females. Here, we apply these empirically derived values investigate of various drive strategies an island population 200,000 using our stochastic, spatially explicit, individual‐based modelling framework. Standard embedded haplosufficient fertility or viability genes failed eradicate, but permanent suppression. In contrast, a two‐target design rescue (HR) haplolethal also targets independent demonstrated considerable suppression potential. Remarkably, HR targeting female showed robust eradication even at relatively low previously rats. Interestingly, rate had influence probability while cutting efficiency was critical. Further, as long latter similar subsequent knockout unlinked gene, then could across range rates. Together, results suggest rates, such have been rats other species, potentially leveraged suppression, offering new opportunities development.

Language: Английский

Citations

0
Diet breadth of a critically endangered owl presents challenges for invasive rodent management: a conservation conundrum DOI Creative Commons
V. Florence Sperring, Andrew R. Weeks,

William David. Webster

et al.

Emu - Austral Ornithology, Journal Year: 2024, Volume and Issue: 124(2), P. 187 - 198

Published: April 2, 2024

Trophic interactions between threatened species complicate management. Similarly, and pest present management challenges, given that control can lead to non-target impacts (e.g. trophic cascades or secondary poisoning). There are records of the critically endangered Norfolk Island Morepork Ninox novaeseelandiae undulata consuming both songbirds invasive rodents subject interventions. Nevertheless, diet morepork remains largely unknown. We visually screened regurgitated pellets using a microscope, alongside environmental DNA (eDNA) screening scats, investigate Morepork. A total 113 19 scats were collected October 2020 June 2021. All moreporks with eDNA metabarcoding had consumed at least one-third samples contained rodents. The owls also found have four five endemic possibly an parrot, most which threatened. Environmental detected more taxa overall, but visual identified greater richness Orthoptera Coleoptera in diet. frequency presents conundrum for conservation managers. Control is considered essential support songbirds, yet this same action places risk poisoning. Urgent investigations needed identify effective methods safe species.

Language: Английский

Citations

3
Public Attitudes towards and Management Strategies for Community Cats in Urban China DOI Creative Commons
Xuan Gu, D. Y. Wu,

Zhang Zi-lin

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(16), P. 2301 - 2301

Published: Aug. 7, 2024

Managing community cats in urban China is a contentious and emerging issue, with debates centering on the most effective humane approaches. This study aimed to investigate public attitudes towards various management strategies. A survey was conducted involving 5382 residents China. Their positive negative roles of areas their support for different methods were examined, including trap-and-kill, taking no action, centralized management, trap-neuter-return (TNR) its variations. Results indicated that 63% participants willing coexist cats, 71% opposed 61% agreed or strongly TNR method Older those higher incomes more likely coexistence cats. In contrast, younger lower-income non-coexistence. Residents first- second-tier cities (e.g., Beijing, Hangzhou, Jinan Cities China) inclined trap-and-kill less than counterparts fourth-tier county-level China). Moreover, lower education supportive action as manage relatively incomes. Those held variations Males females. The implications findings adoption programs are discussed. These novel underscore need targeted educational campaigns promote strategies, addressing concerns cats' welfare. study's insights critical informing policy improving cat

Language: Английский

Citations

2
Broad-scale pesticide screening finds anticoagulant rodenticide and legacy pesticides in Australian frogs DOI Creative Commons
Jodi J. L. Rowley,

Andrew Symons,

Chris Doyle

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 930, P. 172526 - 172526

Published: April 16, 2024

Pesticide contamination poses a significant threat to non-target wildlife, including amphibians, many of which are already highly threatened. This study aims assess the extent pesticide exposure in dead frogs collected during mass mortality event across eastern New South Wales, Australia between July 2021 and March 2022. Liver tissue from 77 individual six species were analysed for >600 legacy contemporary pesticides, rodenticides. More than third (36 %) liver samples contained at least one pesticide, brodifacoum, dieldrin, DDE, heptachlor/heptachlor epoxide, fipronil sulfone, 2-methyl-4-chlorophenoxyacetic acid (MCPA). Brodifacoum, second-generation anticoagulant rodenticide, was found four frog analysed: banjo (Limnodynastes dumerilii), cane toad (Rhinella marina), green tree (Litoria caerulea) Peron's peronii). is first report rodenticide detected wild raising concerns about potential impacts on extending list taxa shown accumulate Dieldrin, banned also two species: striped marsh peronii) caerulea). The toxicological effects these pesticides difficult infer due limited comparable studies; however, low frequency detection presence not considered major contributing factor event. Additional research needed investigate particularly regarding There need continued monitoring improved conservation management strategies mitigation accumulation amphibian populations.

Language: Английский

Citations

1
The missing toxic link: Exposure of non-target native marsupials to second-generation anticoagulant rodenticides (SGARs) suggest a potential route of transfer into apex predators DOI Creative Commons

Kieran Scammell,

Raylene Cooke, Kaori Yokochi

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 933, P. 173191 - 173191

Published: May 12, 2024

Anticoagulant rodenticides (ARs) are used globally to control rodent pests. Second-generation anticoagulant (SGARs) persist in the liver and pose a significant risk of bioaccumulation secondary poisoning predators, including species that do not generally consume rodents. As such, there is clear need understand consumption ARs, particularly SGARs, by non-target consumers determine movement these anticoagulants through ecosystems. We collected analysed livers from deceased common brushtail possums (Trichosurus vulpecula) ringtail (Pseudocheirus peregrinus), native Australian marsupials constitute main diet powerful owl (Ninox strenua), an apex predator significantly exposed SGAR poisoning. ARs were detected 91 % 40 possums. Most detections attributed while first-generation (FGARs) rarely detected. concentrations likely lethal or toxic 42 4 with no effect age, sex, weight either species. There was also landscape type from, suggesting exposure ubiquitous across landscapes. The rate provides insight into pathway which transferred one their key owl. With SGARs entering food-webs species, potential for broader predators greater highlights urgent routine rodenticide testing present as ill found deceased. To limit impact on ecosystem stability use should be regulated governing agencies.

Language: Английский

Citations

1
Investigating the potential of X chromosome shredding for mouse genetic biocontrol DOI Creative Commons
Mark D. Bunting, G.I. Godahewa, Nicole O. McPherson

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: June 12, 2024

Abstract CRISPR-Cas9 technology has facilitated development of strategies that can potentially provide more humane and effective methods to control invasive vertebrate species, such as mice. One promising strategy is X chromosome shredding which aims bias offspring towards males, resulting in a gradual unsustainable decline females. This method been explored insects with encouraging results. Here, we investigated this Mus musculus by targeting repeat DNA sequences on the aim inducing sufficient damage specifically eliminate chromosome-bearing sperm during gametogenesis. We tested three different guide RNAs (gRNAs) repeats chromosome, together male germline-specific promoters for Cas9 expression at stages spermatogenesis. A modest mature Y-bearing was detected some transgenic although did not translate into significant male-biasing offspring. Instead, cleavage meiosis typically resulted spermatogenic block, manifest small testes volume, empty tubules, low concentration, sub/infertility. Our study highlights importance controlling timing activity mammalian spermatogenesis sensitivity spermatocytes disruption.

Language: Английский

Citations

0
Rodenticidas anticoagulantes: una amenaza ignorada para las aves rapaces de Argentina y otros países de Sudamérica DOI Creative Commons
Miguel D. Saggese, Valeria Ojeda, Gala Ortiz

et al.

El Hornero, Journal Year: 2024, Volume and Issue: 39(1), P. 7 - 33

Published: Aug. 15, 2024

En Argentina, el riesgo que los rodenticidas anticoagulantes podrían presentar para las aves rapaces locales fue reconocido inicialmente en la década de 1980. Lamentablemente, 40 años después esta primera señal alarma, siguen siendo ampliamente utilizados país y toda Sudamérica, sin haberse estudiado problema medioambiental estos pueden suponer. Aquí presentamos una revisión práctica e integral sobre intoxicación por rapaces. Discutimos su impacto, tanto individuos como sus poblaciones, también aspectos relacionados al manejo animales intoxicados a necesidad contar con capacidad diagnóstica región. La información aquí recopilada permitirá contenidos relevantes, actualizados accesibles necesarios abordar estudio amenaza conservación Argentina otros países Sudamérica. Al mismo tiempo, esperamos promueva investigaciones tema permitan dar pasos evaluar mitigar uso puede tener otra fauna silvestre

Citations

0