Valorisation of Deinking Paper Sludge for Fertiliser Purposes: New Perspective in Sustainable Agriculture DOI Creative Commons
Halyna Kominko,

Katarzyna Gorazda,

Katarzyna Łoś

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2788 - 2788

Published: Nov. 24, 2024

The growth of the global population, coupled with concomitant economic development, has resulted in generation a substantial quantity waste. transition European Union’s economy towards closed-loop model is prompting comprehensive search for waste management concepts across range industrial sectors. objective this study to valorise deinking paper sludge, which high potential soil formation due its organic matter content. To produce organic–mineral fertiliser, sludge was subjected acid hydrolysis, then neutralised KOH solution and enriched poultry litter ash. final products were characterised terms their nutrient heavy metal bioavailability phosphorus, along forms it occurs fertilisers, determined through implementation five-step fractionation procedure. Furthermore, an eight-week incubation period conducted assess fertilisers’ performance soil. Soil samples tested on weekly basis pH, water-soluble bioavailable phosphorus content using spectroscopic method after previous extraction water Bray’s solution, catalase activity titrimetric method. resulting fertilisers found meet requirements organo-mineral categorised as PK-type total 24.6–39.3%. Fractionation studies demonstrated that contained 20–30% potentially phosphorus. long-term release from confirmed studies. Additionally, observed contribute increase

Language: Английский

Effects of preservatives and drying methods on the nutrient composition and mould counts of hay and pellet processing of Oats DOI Creative Commons
Manlin Wei, Yuxiang Wang, Yufen Zhang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 5, 2025

The aim of this study was to investigate the effects different preservatives and drying methods on nutrient composition mould counts oat hay pellets. Oat pellets were divided into 5 groups: CON (without additives, control), CAP (with 5% calcium propionate), CUR curcumin), SKU Scutellaria baicalensis) KC 2% potassium carbonate). nutrients each group determined after air drying, at 50 °C with forced for 48 h 96 h, respectively. Compared significantly increased dry matter content oat. Under times methods, addition air-drying more effective improving crude protein but not positive In addition, under dried reducing contents neutral detergent fibres acidic fibres. most during in resulted higher CP lower only also which indicates its potential use production.

Language: Английский

Citations

0
Studies on the concerted interaction of microbes in the gastrointestinal tract of ruminants on lignocellulose and its degradation mechanism DOI Creative Commons
Runqi Fu, Han Lin, Qian Li

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 9, 2025

The complex structure of lignocellulose, one the most abundant renewable resources on earth, makes biodegradation challenging. Ruminant gastrointestinal microbiota achieves efficient lignocellulose degradation through a highly synergistic ecosystem, which provides an important research model for sustainable energy development and high value-added chemical production. This review systematically summarizes key mechanisms by ruminant microorganisms, focusing roles rumen hindgut (including cecum, colon, rectum) microorganisms in cellulose, hemicellulose, lignin degradation. study focuses functional differentiation cooperation patterns bacteria, fungi protozoa decomposition, carbohydrate-active enzymes (CAZymes) their new discoveries under histological techniques. In addition, this manuscript explores potential application tract (GIT) microbial improving utilization straw-based feeds. future, revealing mechanism microbe-host synergy integrating multi-omics technologies, ecosystems will provide solutions to promote alleviate global crisis.

Language: Английский

Citations

0
Ensiling Treatment of Rice Straw with Cow Paunch Content to Enhance Bioethanol Production DOI
Dini Dwi Ludfiani, Forita Dyah Arianti, Miranti Dian Pertiwi

et al.

Published: Jan. 1, 2024

Every year, the production of rice straw (RS) and cow paunch contents (PC) is abundant 80% them has not been utilized. This study aimed to determine potency ensiling treatment on in producing bioethanol. The novelty this utilization PC as biomass a source microbial fiber degradation. In study, there were 6 groups treatments. Bioethanol fermentation uses Saccharomyces cerevisiae. Results, significantly affected (p<0.001) changed chemical composition, ethanol was 40.13-102.84 mg/L. Conclude, considered effective ratio for bioethanol production.

Language: Английский

Citations

1
Straw from Different Crop Species Recruits Different Communities of Lignocellulose-Degrading Microorganisms in Black Soil DOI Creative Commons

Chunling Chang,

Yue Guo,

Kuanqiang Tang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 938 - 938

Published: May 5, 2024

The biological degradation of plant residues in the soil or on surface is an integral part natural life cycle annual plants and does not have adverse effects environment. Crop straw characterized by a complex structure exhibits stability resistance to rapid microbial decomposition. In this study, we conducted microcosm experiment investigate dynamic succession community functional characteristics associated with lignocellulose-degrading pathways. Additionally, aimed identify microorganisms from three crop species prevalent Northeast China: soybean (Glycine max Merr.), rice (Oryza sativa L.), maize (Zea mays L.). Our findings revealed that both type time influenced bacterial fungal composition. Metagenome sequencing results demonstrated during degradation, different types assembled carbohydrate-active enzymes (CAZymes) KEGG pathways distinct manners, contributing lignocellulose hemicellulose degradation. Furthermore, isolation microbes yielded 59 14 strains fungi generally exhibiting superior enzyme production compared bacteria. Experiments were assess potential synergistic synthetic communities (SynComs) comprising These SynComs resulted weight loss 42% at 15 days post-inoculation, representing 22% increase conditions without any SynComs. summary, our study provides novel ecological insights into microbes.

Language: Английский

Citations

1
Unlocking the potential of Algerian lignocellulosic biomass: exploring indigenous microbial diversity for enhanced enzyme and sugar production DOI
Samah Chaoua, Sigrid Flahaut, Bertrand Cornu

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(6)

Published: May 25, 2024

Language: Английский

Citations

1
Ensiling treatment of rice straw with cow paunch content to enhance bioethanol production DOI Creative Commons
Dini Dwi Ludfiani, Forita Dyah Arianti, Miranti Dian Pertiwi

et al.

Case Studies in Chemical and Environmental Engineering, Journal Year: 2024, Volume and Issue: 10, P. 100778 - 100778

Published: May 30, 2024

Every year, the production of rice straw and cow paunch content is abundant 80 % them has not been utilized. This study aimed to determine potency ensiling treatment on in producing bioethanol. The novelty this utilization as biomass source microbial fiber degradation. In study, there were six groups treatments. Bioethanol fermentation used Saccharomyces cerevisiae. Results, significantly affected (p < 0.001) changed chemical composition produce ethanol 40.13–102.84 mg/L. Conclude, was considered effective ratio for bioethanol production.

Language: Английский

Citations

1
Development of a Two-Stage Bioprocess for the Production of Bioethanol from the Acid Hydrolysate of Brewer’s Spent Grain DOI Creative Commons

Renata Vičević,

Marko Božinović,

Nikolina Zekić

et al.

Energies, Journal Year: 2024, Volume and Issue: 17(16), P. 3975 - 3975

Published: Aug. 11, 2024

Bioethanol, an alcohol produced by microbial fermentation, is traditionally from sugar-rich plants such as sugar cane, beet and maize. However, there growing interest in the use of lignocellulose, abundant inexpensive renewable energy source, a potential substitute for production biofuels biochemicals. Yeast Saccharomyces cerevisiae, which commonly used ethanol cannot cope with lignocellulose due to lack lignocellulolytic enzymes inefficient functioning pentose phosphate pathway. The aim this research was isolate yeasts that can efficiently produce bioethanol valuable byproducts both glucose xylose two-stage fermentation process using brewer’s spent grains. This approach should maximize utilization improve economic viability while contributing waste valorization sustainability. Kluyveromyces marxianus Candida krusei were identified tested different initial concentrations xylose. results showed but xylose, yielding compounds, 2,3-butanediol glycerol instead. A then carried out weak acidic hydrolysate grain. In first stage, fermented S. cerevisiae bioethanol; second K. C. obtain other products.

Language: Английский

Citations

1
Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting DOI Creative Commons
Jie Li, Juan Li, Ruopeng Yang

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 2107 - 2107

Published: Sept. 16, 2024

The improper treatment of crop straw not only leads to resource wastage but also adversely impacts the ecological environment. However, application microorganisms can accelerate decomposition and improve its utilization. In this study, cellulose-degrading microbial strains were isolated from naturally decayed corn screened using Congo red staining, along with assessing variations in carboxymethyl cellulase (CMCase) activity, filter paper enzyme (FPase) activity β-glucosidase (β-Gase) as well degradation rate. eight strains, namely Neurospora intermedia isolate 29 (A1), Streptomyces FFJC33 (A2), Gibberella moniliformis FKCB-009 (A3), Fusarium fujikuroi EFS3(2) (A4), Fujikuroi FZ04 (A5), Lysine bacillus macroides strain LNHL43 (B1), Bacillus subtilis MPF30 (B2) Paenibacilli lautus ALEB-P1 (C), identified selected for consortium design based on their high activities CMCase, FPase β-Gase. fungi, bacteria actinomycete combined without antagonistic effects decomposition. results showed A2B2 combination had a significantly higher at 55.44 U/mL β-Gase 25.73 than other two combinations (p < 0.05). Additionally, rate was 40.33%, which considerably that strains/consortia. A4B2C superior including CMCase value 35.03 U/mL, 63.59 26.15 different those three Furthermore, seven single construct various consortiums situ composting order evaluate potential. Taken whole, composting, temperature, moisture content, pH, E4/E6 seed germination index, indicated consisting intermediate 29, EFS3(2), FZ04, Lysinibacillus macrolides, sphaericus, Paenibacillus advantageous yielded high-quality compost. flora able effectively degrade straw. This study provides novel solution construction

Language: Английский

Citations

1
Isolation, Characterization, and Optimization of Culture Medium for Local Straw-Degrading Bacteria from Northeastern Black Soils of China DOI Creative Commons
Lei Zhang, Tianyu Liu,

Chunjuan Yan

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2591 - 2591

Published: Nov. 3, 2024

In order to solve the problem of low and poor straw degradation in typical black soil areas Northeast China, present study was carried out screen potential situ strains with cellulose ability from soils China play a role resourceful utilization development sustainable agriculture. The evaluated by combining sodium carboxymethyl plate screening cellulase viability assay; species identification morphology, physiology, biochemistry, molecular biology; basic medium formulation optimized Box–Behnken response surface methodology. Ten cellulose-degrading were identified: ZL-5, ZL-69, ZL-88, ZL-95, ZL-111, ZL-137, ZL-139, ZL-140, ZL-187, ZL-216, which ZL-139 had highest production capacity, secretion 7.8781 U/mL enzyme-producing medium. identified as Bacillus cereus; best glucose—4.284 g/L, yeast extract—1.454 MgSO4—0.417 KH2PO4—0.5 K2HPO4—1.5 NaCl—1.0 g/L. conclusion, strain has good for crop can be candidate straw-rotting agent northeast promising prospects utilization.

Language: Английский

Citations

1
Molecular Cloning, In Silico Analysis, and Characterization of a Novel Cellulose Microfibril Swelling Gene Isolated from Bacillus sp. Strain AY8 DOI Creative Commons
Md. Azizul Haque, Dhirendra Nath Barman, Aminur Rahman

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2857 - 2857

Published: Nov. 24, 2023

A novel cellulose microfibril swelling (Cms) gene of Bacillus sp. AY8 was successfully cloned and sequenced using a set primers designed based on the conserved region from genomic database. The molecular cloning Cms revealed that consisted 679 bp sequences encoding 225 amino acids. Further in silico analysis unveiled contained NlpC/P60 exhibited homology 98% with family proteins found both strains, Burkholderialata Burkholderia vietnamiensis. recombinant enzyme had significant impact reduction crystallinity indices (CrI) various substrates including 3%, 3.97%, 4.66%, substantial 14.07% for filter paper, defatted cotton fiber, avicel, alpha cellulose, respectively. Additionally, notable changes spectral features were observed among treated enzymes compared to untreated control. Specifically, there decrease band intensities within regions 3000-3450 cm-1, 2900 1429 1371 cm-1 Furthermore, maximum activity at pH 7.0 along temperature 40 °C. docking data ligand molecules, such as cellobiose, dextrin, maltose 1-phosphate, feruloyated xyloglucan, effectively bonded active site enzyme. dynamics simulations displayed stable interactions cellobiose dextrin molecules up 100 ns. It is noteworthy mention did not match those bioadditives like expansins swollenin proteins. This study initial report bacterial swellase enzyme, which could potentially serve an additive enhance biofuel production by releasing fermentable sugars cellulose.

Language: Английский

Citations

1