SARS-CoV-2 RNA Detection in Wastewater and Its Effective Correlation with Clinical Data during the Outbreak of COVID-19 in Salamanca DOI Open Access
Ángel Emilio Martínez de Alba, María E. Morán-Diez, Juan C. García-Prieto

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8071 - 8071

Published: July 24, 2024

Wastewater treatment plants (WWTPs) are the final stage of anthropogenic water cycle where a wide range chemical and biological markers human activity can be found. In COVID-19 disease contexts, wastewater surveillance has been used to infer community trends based on viral abundance SARS-CoV-2 RNA variant composition, which served anticipate establish appropriate protocols prevent potential outbreaks. Numerous studies worldwide have provided reliable robust tools detect quantify in wastewater, although due high dilution degradation rate such samples, detection limit pathogen bottleneck for proposed so far. The current work provides comprehensive systematic study different parameters that may affect hinder its quantification. results obtained using synthetic as template allow us consider 10 genome copies per µL is minimum concentration consistent values quantification RNA. RT-qPCR analysis samples collected at WWTP Salamanca (western Spain) six pumping stations city showed below this threshold, positive must confirmed by sequencing identify specific sequence. This allowed find correlations between levels found clinical data reported health authorities. close match environmental from case similar experimental approaches four other cities same region. present methodological approach reinforces usefulness wastewater-based epidemiology (WBE) face future pandemic

Language: Английский

Wastewater Surveillance for Poliovirus in Selected Jurisdictions, United States, 2022–2023 DOI Creative Commons
Erin Whitehouse, Nancy Gerloff,

Randall English

et al.

Emerging infectious diseases, Journal Year: 2024, Volume and Issue: 30(11)

Published: Oct. 23, 2024

Wastewater testing can inform public health action as a component of polio outbreak response. During 2022-2023, total 7 US jurisdictions (5 states and 2 cities) participated in prospective or retrospective wastewater for poliovirus after paralytic case was identified New York state. Two distinct vaccine-derived type viruses were detected from state City during 2022, representing separate importation events. Of those viruses, 1 resulted persistent community transmission multiple counties case. No the other participating (Connecticut, Jersey, Michigan, Illinois Chicago, IL). The value routine surveillance apart an is unclear. However, these results highlight ongoing risk importations into United States need to identify undervaccinated communities increase vaccination coverage prevent polio.

Language: Английский

Citations

4
Wastewater-based analysis of antimicrobial resistance at UK airports: Evaluating the potential opportunities and challenges DOI Creative Commons
Margaret E. Knight, Kata Farkas, Matthew J. Wade

et al.

Environment International, Journal Year: 2025, Volume and Issue: 195, P. 109260 - 109260

Published: Jan. 1, 2025

Language: Английский

Citations

0
Participación Ciudadana para la dinamización de la Gestión de Residuos Sólidos Municipales de Moche, Trujillo DOI Creative Commons
Francisco Alejandro Espinoza Polo, Gaby Esther Chunga Pingo, Carlos Alfredo Cerna Muñóz

et al.

EVSOS, Journal Year: 2024, Volume and Issue: 2(3), P. 151 - 175

Published: March 1, 2024

Este estudio tuvo como propósito determinar el efecto de la colaboración ciudadana en administración los desechos sólidos Municipalidad Moche, Trujillo. El es carácter vinculativo explicativo, con bosquejo básico transeccional descriptivo vinculacional causal y se empleó un enfoque orientación cuantitativa. La población integrada por 31,500 pobladores que residen zona urbana Moche. selección muestral fue conformidad e interés investigadores. proceso acopio datos encuesta, crearon dos herramientas para tal fin, cuales fueron: herramienta mensurar participación gestión residuos sólidos. Los resultados encontrados este han establecido existe nexo enormemente significativo positivo entre sólidos; ya ha anotado índice vinculación Rho Spearman r=0,589**; también primera variable segunda variable. Como conclusión indispensable fortalecer acciones centradas acrecentar

Citations

0
SARS-CoV-2 RNA Detection in Wastewater and Its Effective Correlation with Clinical Data during the Outbreak of COVID-19 in Salamanca DOI Open Access
Ángel Emilio Martínez de Alba, María E. Morán-Diez, Juan C. García-Prieto

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8071 - 8071

Published: July 24, 2024

Wastewater treatment plants (WWTPs) are the final stage of anthropogenic water cycle where a wide range chemical and biological markers human activity can be found. In COVID-19 disease contexts, wastewater surveillance has been used to infer community trends based on viral abundance SARS-CoV-2 RNA variant composition, which served anticipate establish appropriate protocols prevent potential outbreaks. Numerous studies worldwide have provided reliable robust tools detect quantify in wastewater, although due high dilution degradation rate such samples, detection limit pathogen bottleneck for proposed so far. The current work provides comprehensive systematic study different parameters that may affect hinder its quantification. results obtained using synthetic as template allow us consider 10 genome copies per µL is minimum concentration consistent values quantification RNA. RT-qPCR analysis samples collected at WWTP Salamanca (western Spain) six pumping stations city showed below this threshold, positive must confirmed by sequencing identify specific sequence. This allowed find correlations between levels found clinical data reported health authorities. close match environmental from case similar experimental approaches four other cities same region. present methodological approach reinforces usefulness wastewater-based epidemiology (WBE) face future pandemic

Language: Английский

Citations

0