Antimicrobial Resistance in Diverse Ecological Niches—One Health Perspective and Food Safety

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 443 - 443

Published: April 28, 2025

Antimicrobial resistance (AMR) is a multi-sectoral, systemic, and global issue worldwide. use (AMU) key factor in the selection of resistant bacteria within different ecological niches, from agriculture to food-producing animals humans. There question regarding extent which antibiotics livestock production primary food sector influences transmission and/or genes throughout chain thus contributes complexity development AMR Although trends prevalence foodborne pathogens have changed over time, burden niches with genes, primarily commensal microorganisms, concern. The implementation harmonized surveillance AMU would provide comprehensive insights into actual status further interventions leading its reduction. Tracking by applying advanced genome-based techniques developing shared data repositories strengthen One Health concept.

Language: Английский

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Mitigating the transfer risk of antibiotic resistance genes from fertilized soil to cherry radish during the application of insect fertilizer

Environment International, Journal Year: 2025, Volume and Issue: unknown, P. 109510 - 109510

Published: April 1, 2025

Language: Английский

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Antibiotics Uptake from Soil and Translocation in the Plants – Meta-analysis

CHIMIA International Journal for Chemistry, Journal Year: 2024, Volume and Issue: 78(4), P. 209 - 214

Published: April 24, 2024

Antibiotics reach agricultural soils via fertilization with manure and biosolids as well irrigation withwastewater have the potential to be taken up by growing crops. The fate of antibiotics in terms uptakefrom soil plants, translocation from root leaves, is determined a combination antibiotic’sphysio-chemical (e.g. speciation, lipophilicity), organic carbon content, pH) plant (e.g.transpiration rates) characteristics. In this meta-analysis, literature search was executed obtain an overview antibiotic uptake aim identify patterns different classes. Overall, we found that higher tetracyclines leaves observed compared sulfonamides. Differences were also within where roots close equal concentrations, while sulfonamides represented tendency accumulate fraction. antibiotic’s characteristics high influence on their fate, for example, water-solubility uncharged speciation typical pH ranges likely induces tetracycline plant. Despite advances knowledge over past decade, our meta-analysis indicated available research focused limited number analytes Furthermore, fastgrowing species spinach, lettuce, radish) are overly studies crop significance human food sources corn, wheat, potato), requiring more attention future research.

Language: Английский

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Extended-spectrum ß-lactamase (ESBL)- and carbapenemase-producing Enterobacterales isolated from fresh herbs and salads at retail level in Switzerland

Journal of Food Protection, Journal Year: 2024, Volume and Issue: 87(11), P. 100368 - 100368

Published: Oct. 2, 2024

Fresh produce is usually consumed raw or minimally processed, making it a potential vehicle for the transmission of antimicrobial-resistant (AMR) microorganisms to humans. The objective study was assess occurrence extended-spectrum ß-lactamase (ESBL)- and carbapenemase-producing Enterobacterales (ESBL-E CPE), respectively, in 118 fresh herbs 101 bagged salads collected at retail level Switzerland characterize isolates' phenotypic genotypic properties using culture-based methods whole genome sequencing (WGS). Of herbs, 6/118 contained ESBL-E 7/118 yielded CPE. salads, 13/101 1/101 Antimicrobial susceptibility testing (AST) identified 9/29 isolates as multidrug-resistant (MDR). were Escherichia coli (n = 6), Klebsiella pneumoniae 4) Enterobacter chuandaensis 1), Kluyvera spp. 1) carrying (bla) genes belonging cefotaximase-München (bla

Language: Английский

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Optimization of the Method for Isolating Bacterial DNA from the Aboveground Part of Lettuce

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8513 - 8513

Published: Aug. 4, 2024

Developing an effective method for isolating bacterial genetic material from plants is a relatively challenging task and often does not yield adequately prepared further analyses. Previous studies overlook connections, primarily focusing on laboratory investigations. With advancements in high-throughput sequencing techniques, we can now revisit delve deeper into these interactions. Our study focuses the initial phase of investigations: isolation. Extracting DNA aboveground plant parts, known as phyllosphere, poses significant challenge due to plant-derived contaminants. Existing isolation protocols frequently inconsistent results, necessitating continuous refinement optimization. In our study, developed protocol employing mechanical–chemical lysis, sonication, membrane filtration. This approach yielded high-quality at concentration 38.08 ng/µL, suitable advanced applications. results underscore effectiveness necessity methods conducting comprehensive microbiological Furthermore, research only lays groundwork lettuce microbiota, but also highlights potential utilizing investigating other their microbiomes.

Language: Английский

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Unde venis? Bacterial resistance from environmental reservoirs to lettuce: tracking microbiome and resistome over a growth period

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Abstract Fresh produce is suggested to contribute highly shaping the gut resistome. We investigated impact of pig manure and irrigation water quality on microbiome resistome field-grown lettuce over an entire growth period. Lettuce was grown under four regimes, combining soil amendment with (with/without) sprinkler using river upstream wastewater input, disinfected by UV (with/without). leaves, soil, samples were collected weekly analyzed bacterial cultivation, 16S rRNA gene amplicon sequencing, shotgun metagenomics from total community DNA. Cultivation yielded only few clinically relevant antibiotic-resistant bacteria (ARB), but numbers ARB increased time, while no treatment-dependent changes observed. Microbiome analysis confirmed a temporal trend. Antibiotic resistance genes (ARGs) unique included multidrug β-lactam ARGs, whereas uniquely shared mainly glycopeptide tetracycline ARGs. Surface carried (e.g. ESBL-producing Escherichia coli or Serratia fonticola) without affecting overall significantly. Resistance markers including biocide metal in manure, especially young (increased contact). Overall, all environments had their share as sources resistome, main source plants. therefore suggest minimizing soil-vegetable contact minimize fresh produce.

Language: Английский

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Profiling the Enterobacterales community isolated from retail foods in England

Journal of Food Protection, Journal Year: 2024, Volume and Issue: 87(11), P. 100369 - 100369

Published: Oct. 2, 2024

Enterobacterales include foodborne pathogens of importance to public health and are often targeted in food surveillance programs as both safety hygiene indicators. Furthermore, important the context antimicrobial resistance dissemination, also impacting infection treatment efficacy. In this study, prevalence characteristics UK retail foods were examined. From 110 samples, 253 recovered, with 16S rRNA sequencing revealing a diverse species community, including enteropathogens; most common Proteus mirabilis Escherichia coli (18% each). Antimicrobial was common, 160/253 (63%) isolates being resistant at least 1 antimicrobial. Resistance all tested antimicrobials observed. Thirteen percent multidrug resistant, 2 each 8 or 9 tested. Klebsiella possessed relatively higher levels other species. Hafnia, Kluyvera, produced significantly biofilm biomass than (p = 0.038, 0.028, 0.042, respectively) 0.001, 0.008, respectively). Simultaneous curli fimbriae cellulose production noted 7% 37 °C, but not 15 °C. This research demonstrates high diversity within foods, alongside notable phenotypes enteropathogenic species, highlighting need for effective interventions.

Language: Английский

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Prevalence of Antibiotic Resistance Genes in Differently Processed Smoothies and Fresh Produce from Austria

Foods, Journal Year: 2024, Volume and Issue: 14(1), P. 11 - 11

Published: Dec. 25, 2024

Plant-derived foods are potential vehicles for microbial antibiotic resistance genes (ARGs), which can be transferred to the human microbiome if consumed raw or minimally processed. The aim of this study was determine prevalence and amount clinically relevant ARGs mobile genetic elements (MGEs) in differently processed smoothies (freshly prepared, cold-pressed, pasteurized high-pressure processed) fresh produce samples (organically conventionally cultivated) assess health hazards associated with their consumption. MGE ISPps class 1 integron-integrase gene intI1 were detected by probe-based qPCR concentrations up 104 copies/mL all smoothies, lettuce, carrots a single tomato sample. highest total (2.2 × 105 copies/mL) most diverse ARG loads (16/26 targets) observed freshly prepared lowest prevalences (5/26) (4.1 103 high-pressure-processed (HPP) smoothies. BlaCTX-M-1-15 (1.2 c/mL) strB (6.3 abundant, qacEΔ1 (95%), blaTEM1 (85%), ermB sul1 (75%, each) prevalent ARGs. QnrS, vanA, sat-4, blaKPC, blaNDM-1 blaOXA-10 never detected. HPP treatment reduced ca. 5 logs, also destroying extracellular DNA potentially encoding that could otherwise bacterial transformation. microbiome, pathogens, carriers competent bacteria able take identified Illumina 16S rRNA sequencing. To reduce risk AMR spread from our data endorse application DNA-disintegrating processing techniques such as HPP.

Language: Английский

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Bacterial contamination and associated antibiotic resistance in Fresh Produce : A comparative study between retail outlets and local markets

Journal Of Advanced Zoology, Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 29, 2023

The consumption of food is essential for human survival, but the presence bacterial pathogens in fresh produce can pose significant risks to public health by causing severe foodborne and enteric diseases. With this concern mind, our study aimed assess contamination levels specific species, E. coli, Salmonella spp., Staphylococcus aureus, various items (pear, guava, apple, carrot, turnip, cucumber) sourced from both local markets retail outlets. Additionally, we investigated antibiotic resistance these species. Our findings revealed selected S. all samples obtained outlets markets. Notably, among markets, pear exhibited highest load (4.29 × 105 CFU/gm) compared guava (3.16 apple (2.83 CFU/gm). Among vegetables, carrots demonstrated (4.27 turnip (3.38 cucumber (2.45 Similar pattern was also common Pear itself as most contaminated fruit (2.13 CFU/gm), followed guavas (1.43 apples (0.882 maximum (2.31 (1.05 (0.689 Comparing microbes available produce, it observed that coli consistently showed abundance amongst species cultivated Alarmingly, isolated displayed substantial commonly used broad-spectrum antibiotics, emphasizing inherent posed present on raw produce. This underscores potential threat consumer upon such We provide scientific evidence prove may serve a vehicle exposure antimicrobial-resistant bacteria. It's global alarming situation, which needs immediate attentions steps control. Concerted efforts should be made mitigate resistant bacteria at stages chain, production consumption. To achieve goal, good agricultural practices employed regulatory authorities need address problem immediately

Language: Английский

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