iScience, Journal Year: 2024, Volume and Issue: 28(2), P. 111720 - 111720
Published: Dec. 31, 2024
Language: Английский
Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 623 - 623
Published: April 26, 2025
Acinetobacter baumannii and Klebsiella pneumoniae are significant nosocomial pathogens worldwide. In this study, the general characterization of A. K. isolates obtained from blood intensive care unit patients multidisciplinary scientific practical center emergency medicine January to September 2024 was performed. Prophage regions prophage-derived tailspike polysaccharide-depolymerizing or -modifying enzymes within these were identified characterized in detail using a refined workflow. The protocol, encompassing comprehensive survey all predicted bacterial proteins, revealed an average 6.0 prophage per genome, including putatively derived filamentous phages, 4.8 isolate. Analysis putative indicated that most related previously isolated, yet unclassified, temperate phages infecting pneumoniae. However, certain sequences likely originated representing novel groups comparatively distant known phages.
Language: Английский
Citations
0
Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33372 - e33372
Published: June 21, 2024
Acinetobacter baumannii, a notorious opportunistic pathogen, presents formidable challenge in both clinical and environmental fields due to its resilience ability acquire resistance. This study undertook comprehensive analysis of 183 A. baumannii isolates collected between 2019 2022 from intra-hospital infections (IHI), hospital sewages (Hs), wastewater treatment plants (WWTP), adjacent river waters two Southern cities, focusing on their resistome, virulome, mobilome through isolation chromogenic media, identification by MALDI-TOF-MS antibiotic susceptibility testing disk diffusion) followed genotypic characterization [whole-genome sequencing (WGS), 3rd generation the MinION (ONT) platform, pangenome description, respectively horizontal gene transfer conjugation assays]. Our findings reveal significant genomic plasticity prevalence high-risk international clones, underlining potential these act as reservoirs for resistance genes (ARGs) that could be dynamically exchanged settings mobile genetic elements (MGEs) such pMAL1 plasmids critical role WWTPs persistence spread baumannii. Moreover, our first report co-occurrence blaOXA-23 blaOXA-72 ST2 clone. Thus, research underscores necessity integrated surveillance targeted interventions across healthcare sectors mitigate risk posed this adaptable pathogen.
Language: Английский
Citations
3
Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 205, P. 107610 - 107610
Published: April 17, 2025
Language: Английский
Citations
0
Frontiers in Public Health, Journal Year: 2024, Volume and Issue: 12
Published: Oct. 9, 2024
Introduction The extent to which sinks are contaminated by carbapenem-resistant Acinetobacter baumannii (CRAB) in intensive care units (ICUs) and the association between these hospital-acquired CRAB infections during non-cluster period remains largely unknown. Here, we performed a prospective multicenter study 16 ICUs at 11 tertiary hospitals Chengdu, China. Methods We sampled sinks, collected clinical isolates, conducted whole-genome sequencing analysis. Results A total of 789 swabs were from 158 isolates recovered resulting contamination rate 10.16%. Twenty-seven period. majority (97.67%, 42/43) belonged ST2, 36 (83.72%) them had both bla OXA-23 OXA-66 . 43 strains 12 clones. One certain clone caused multiple contaminations seven one GICU. Two clones ST2 -carrying sink likely sources two clusters GICUs, respectively. Five found be common but hospitals. Conclusion handwashing is high some local ICUs, can serve as environmental reservoirs for clusters.
Language: Английский
Citations
0
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177696 - 177696
Published: Nov. 23, 2024
Language: Английский
Citations
0
mSphere, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 31, 2024
ABSTRACT Acinetobacter, particularly the Acinetobacter baumannii group, is a major cause of nosocomial infections, and carbapenem-resistant spp. are important human pathogens. We collected 492 strains from two hospitals in Taiwan classified them using MALDI-TOF MS bla OXA-51-like PCR; 94.5% were A. baumannii, 5.5% non- A . (NAB). confirmed their identity by rpo B gene sequencing 239 randomly selected all 27 NAB strains. Our analysis revealed that alleles OXA51-like-negative matched those species, seifertii nosocomialis , while OXA51-like-positive as per Pasteur MLST scheme database. Among strains, 240 exhibited carbapenem resistance, including 237 (CRAB) three CR-NAB All CRAB positive for ; 72.6% also carried OXA-23-like 22.8% OXA-24-like 3.4% co-carried + 1.27% alone. one NDM-1 OXA-58-like IMP established new multiplex PCR method rapid screening common capsular types (KL), which showed difference between carbapenem-susceptible (CSAB). KL2, KL10, KL22, KL52 accounted 76.6% whereas about half CSAB other KL types. Of remaining KL14 was most predominant type at 10.3%. further conducted typing 262 isolates found carbapenemase genes correlated with either ST or Additionally, correlations types, certain clinical records. Whole-genome -carrying strain plasmid transferable via vitro conjugation, suggesting may be reservoir plasmids. IMPORTANCE Carbapenem-resistant have been identified World Health Organization top priority antibiotic development. KL-typing efficient to enable epidemiological surveillance provide foundation effective infection control. investigation molecular epidemiology group prevalence among CR CS NAB. an carrying Ti-type conjugative operon on small harbored encoding alongside conferring resistance aminoglycosides bleomycin closely resembled sequences detected soli pittii China, respectively, its potential transmitting multidrug contributing spread antimicrobial resistance.
Language: Английский
Citations
0
iScience, Journal Year: 2024, Volume and Issue: 28(2), P. 111720 - 111720
Published: Dec. 31, 2024
Language: Английский
Citations
0