Maximizing viral nucleic acid yield from passive samplers: Evaluating elution and extraction protocols DOI Creative Commons
Madison T. Gouthro, Emalie K. Hayes, Graham A. Gagnon

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177834 - 177834

Published: Nov. 30, 2024

The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their ability recover SARS-CoV-2 bacteriophage MS2 from samplers. Promega Wizard® Enviro Total Nucleic Acid Kit, paired with a Tween®20-based buffer, provided highest virus efficiency sampling. Field-scale applications demonstrated effectiveness samplers capturing SARS-CoV-2, INFA, RSV, MeV using optimized protocols. combination Tween®20 based buffer kit led increased detection frequencies grab samples, which remained lower than underscores importance selecting appropriate TNA maximize samples. By optimizing these protocols, we enhance sensitivity reliability ecosystems.

Language: Английский

From capture to detection: A critical review of passive sampling techniques for pathogen surveillance in water and wastewater DOI Creative Commons
Emalie K. Hayes, Graham A. Gagnon

Water Research, Journal Year: 2024, Volume and Issue: 261, P. 122024 - 122024

Published: June 30, 2024

Water quality, critical for human survival and well-being, necessitates rigorous control to mitigate contamination risks, particularly from pathogens amid expanding urbanization. Consequently, the necessity maintain microbiological safety of water supplies demands effective surveillance strategies, reliant on collection representative samples precise measurement contaminants. This review critically examines advancements passive sampling techniques monitoring in various systems, including wastewater, freshwater, seawater. We explore evolution conventional materials innovative adsorbents pathogen capture shift culture-based molecular detection methods, underscoring adaptation this field global health challenges. The comparison highlights sampling's efficacy over like grab its potential overcome existing challenges through use such as granular activated carbon, thermoplastics, polymer membranes. By evaluating literature, work identifies standardization gaps proposes future research directions augment efficiency, specificity, utility environmental public surveillance.

Language: Английский

Citations

11
State-space modelling using wastewater virus and epidemiological data to estimate reported COVID-19 cases and the potential infection numbers DOI
Syun-suke Kadoya, Yubing Li, Yilei Wang

et al.

Journal of The Royal Society Interface, Journal Year: 2025, Volume and Issue: 22(222)

Published: Jan. 1, 2025

The current situation of COVID-19 measures makes it difficult to accurately assess the prevalence SARS-CoV-2 due a decrease in reporting rates, leading missed initial transmission events and subsequent outbreaks. There is growing recognition that wastewater virus data assist estimating potential infections, including asymptomatic unreported infections. Understanding hidden behind reported cases critical for decision-making when choosing appropriate social intervention measures. However, models implicitly assume homogeneity human behaviour, such as shedding patterns within population, making challenging predict emergence new variants variant-specific or parameters. This can result predictions with considerable uncertainty. In this study, we established state-space model based on viral load both infection numbers. Our using showed high goodness-of-fit case numbers despite dataset waves two distinct variants. Furthermore, successfully provided estimates infection, reflecting superspreading nature transmission. study supports notion surveillance modelling have effectively

Language: Английский

Citations

2
Disinfection effectiveness and mechanism of in-duct microwave radiation on A. versicolor adhere to a SiC composite filter DOI
Yuan Zhang, Tian Yan,

Zhaowei Xu

et al.

International Journal of Ventilation, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: Jan. 28, 2025

Language: Английский

Citations

0
Improved passive sampling methods for wastewater to enable more sensitive detection of SARS-CoV-2 and its variants DOI
Md Alamin,

Pelumi Oladipo,

James Hartrick

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175044 - 175044

Published: July 27, 2024

Language: Английский

Citations

1
Maximizing viral nucleic acid yield from passive samplers: Evaluating elution and extraction protocols DOI Creative Commons
Madison T. Gouthro, Emalie K. Hayes, Graham A. Gagnon

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177834 - 177834

Published: Nov. 30, 2024

The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly non-enteric viruses. Despite advances wastewater monitoring, surveillance of viruses freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive protocols by determining optimal extraction and elution methods enhancing recovery nucleic acids freshwater. Three commercially available total acid (TNA) kits four buffers were assessed their ability recover SARS-CoV-2 bacteriophage MS2 from samplers. Promega Wizard® Enviro Total Nucleic Acid Kit, paired with a Tween®20-based buffer, provided highest virus efficiency sampling. Field-scale applications demonstrated effectiveness samplers capturing SARS-CoV-2, INFA, RSV, MeV using optimized protocols. combination Tween®20 based buffer kit led increased detection frequencies grab samples, which remained lower than underscores importance selecting appropriate TNA maximize samples. By optimizing these protocols, we enhance sensitivity reliability ecosystems.

Language: Английский

Citations

0