Taxonomic variation, plastic degradation, and antibiotic resistance traits of plastisphere communities in the maturation pond of a wastewater treatment plant DOI Creative Commons
Stefan D. M. Maday, Joanne M. Kingsbury,

Louise Weaver

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

ABSTRACT Wastewater treatment facilities can filter out some plastics before they reach the open environment, yet microplastics often persist throughout these systems. As age, in wastewater may both leach and sorb pollutants fragment to provide an increased surface area for bacterial attachment conjugation, possibly impacting antimicrobial resistance (AMR) traits. Despite this, little is known about effects of persistent plastic pollution on microbial functioning. To address this knowledge gap, we deployed five different artificially weathered types a glass control into final maturation pond municipal plant Ōtautahi-Christchurch, Aotearoa/New Zealand. We sampled plastic-associated biofilms (plastisphere) at 2, 6, 26, 52 weeks, along with ambient water, three depths (20, 40, 60 cm from water surface). investigated changes plastisphere diversity functional potential through metagenomic sequencing. Bacterial 16S ribosomal RNA genes composition did not vary among controls ( P = 0.997) but varied sampling times [permutational multivariate analysis variance (PERMANOVA), 0.001] (PERMANOVA, 0.011). Overall, there was no polymer-substrate specificity evident total 0.67), time 0.002) depth were significant factors 0.001). The housed diverse AMR gene families, potentially influenced by biofilm-meditated conjugation. also harbored abundance associated biodegradation nylon, or nylon-associated substances, including nylon oligomer-degrading enzymes hydrolases. IMPORTANCE Plastic pervasive ubiquitous. Occurrences causing entanglement ingestion, leaching toxic additives organic environmental plastics, their consequences marine macrofauna are widely reported. However, Shotgun metagenomics sequencing provides us necessary tools examine broad-scale community functioning further investigate how influence communities. This study insight consequence continued exposure waste comparing prokaryotic [linear low-density polyethylene (LLDPE), nylon-6, terephthalate, polylactic acid, oxygen-degradable LLDPE], glass, over 12 months cm) within tertiary plant.

Language: Английский

Taxonomic variation, plastic degradation, and antibiotic resistance traits of plastisphere communities in the maturation pond of a wastewater treatment plant DOI Creative Commons
Stefan D. M. Maday, Joanne M. Kingsbury,

Louise Weaver

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

ABSTRACT Wastewater treatment facilities can filter out some plastics before they reach the open environment, yet microplastics often persist throughout these systems. As age, in wastewater may both leach and sorb pollutants fragment to provide an increased surface area for bacterial attachment conjugation, possibly impacting antimicrobial resistance (AMR) traits. Despite this, little is known about effects of persistent plastic pollution on microbial functioning. To address this knowledge gap, we deployed five different artificially weathered types a glass control into final maturation pond municipal plant Ōtautahi-Christchurch, Aotearoa/New Zealand. We sampled plastic-associated biofilms (plastisphere) at 2, 6, 26, 52 weeks, along with ambient water, three depths (20, 40, 60 cm from water surface). investigated changes plastisphere diversity functional potential through metagenomic sequencing. Bacterial 16S ribosomal RNA genes composition did not vary among controls ( P = 0.997) but varied sampling times [permutational multivariate analysis variance (PERMANOVA), 0.001] (PERMANOVA, 0.011). Overall, there was no polymer-substrate specificity evident total 0.67), time 0.002) depth were significant factors 0.001). The housed diverse AMR gene families, potentially influenced by biofilm-meditated conjugation. also harbored abundance associated biodegradation nylon, or nylon-associated substances, including nylon oligomer-degrading enzymes hydrolases. IMPORTANCE Plastic pervasive ubiquitous. Occurrences causing entanglement ingestion, leaching toxic additives organic environmental plastics, their consequences marine macrofauna are widely reported. However, Shotgun metagenomics sequencing provides us necessary tools examine broad-scale community functioning further investigate how influence communities. This study insight consequence continued exposure waste comparing prokaryotic [linear low-density polyethylene (LLDPE), nylon-6, terephthalate, polylactic acid, oxygen-degradable LLDPE], glass, over 12 months cm) within tertiary plant.

Language: Английский

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