Surveillance and Characterization of Carbapenem-resistant Enterobacter cloacae Complex from China, 2015-2018 DOI Creative Commons
Kun Ye, Yongqing Zhang, Xuemei Qiu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Abstract Background: The carbapenem-resistant Enterobacter cloacae (CREC) has become a global health concern. However, our understanding of its epidemiological trends and resistance mechanisms still needs to be improved. Methods: 92 non-repetitive clinical CREC isolates were collected from 12 hospitals across China identified using MALDI-TOF MS, rMLST, ANI, MASH, dDDH. Minimal inhibitory concentrations determined via broth microdilution. Resistance genes plasmid replicons ResFinder PlasmidFinder. Carbonyl cyanide 3-chlorophenylhydrazone suppression test OmpC OmpF overexpression conducted on 32 non-carbapenemase-producing (NCP) isolates. A phylogenetic tree was constructed with kSNP4 visualized through iTOL. Results: Among the isolates, E. hormaechei predominate (76 isolates), followed by kobei (five isolates). Within hormaechei, subsp xiangfangensis most widespread subspecies. Sixty produced carbapenemase, blaNDM-1 in 36 blaKPC-2 six isolates, and blaNDM-5 Two harbored both blaKPC-2. Thirty-two NCP primarily due carbonyl suppression. Conclusions: China, dominant carbapenemase-coding gene. For efflux pumps main reason for carbapenem resistance, susceptibility could restored an antibiotic-free environment.

Language: Английский

Clinical Characteristics and Risk Factors for Multidrug-Resistant Enterobacter cloacae Complex Bacteremia in a Chinese Tertiary Hospital: A Decade Review (2013–2022) DOI Creative Commons
Mei Han, Miao‐Miao Hua,

Hui Xie

et al.

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 427 - 440

Published: Jan. 1, 2025

Objective: This study aimed to analyze the antimicrobial resistance profiles, clinical characteristics and risk factors of bacteremia caused by Enterobacter cloacae complex (ECC) strains.Methods: We retrospectively collected data from patients diagnosed with ECC between 2013 2022 in a tertiary hospital Jiangsu.Subgroup analyses were performed based on multidrug (MDR), nosocomial acquisition, polymicrobial bacteremia, mortality.Results: Among 188 strains, highest was ceftriaxone (39.9%), followed ceftazidime (36.7%) aztreonam (31.2%), low carbapenems (<8.6%) amikacin (1.6%).MDR accounted for 30.9% (58/188).Previous antibiotic therapy an independent factor MDR (OR = 3.193, P < 0.020), while appropriate significantly reduced 0.279, 0.001).ICU admission both endoscopy blood transfusion associated mortality.Conclusion: Carbapenems are most effective treatments bacteremia.Previous increases ECC, reduces it.ICU is linked higher mortality.Effective control requires comprehensive strategies, including detection, identification, infection prevention.

Language: Английский

Citations

0
Insertion sequences in mgrB and mutations in two-component system genes confer high polymyxin resistance to carbapenem-resistant Enterobacter cloacae complex strains DOI Creative Commons

Jiming Wu,

Jisheng Zhang,

Jianmin Wang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 17, 2025

Due to the complexity of identifying Enterobacter cloacae complex (ECC) at species level, little is known about distribution carbapenem-resistant ECC (CRECC). Plasmid-mediated mcr family genes are significant contributors polymyxin resistance. The emergence mcr-9 gene has further complicated landscape resistance in CRECC. Our study aimed ascertain prevalence CRECC and gene, elucidate mechanisms underlying high-level B (PB). In this study, we collected 212 non-replicating strains, 38 strains (17.9%, 38/212) hormaechei (71.1%, 27/38) as predominant endemic strains. Among these, 10 (36.3%, 10/38) were found harbor gene. Interestingly, presence did not significantly impact PB or impose a fitness cost. While overexpression can enhance within certain range may incur costs, it does result 17 was notably increased (from 16 128 mg/L), accompanied by mutations phoP/Q mgrB genes. Notably, two novel insertion sequences, IS5D IS1X2, discovered inactivation results loss its negative regulatory effect on two-component system. Protein structure predictions indicated that phoQ primarily affect phosphatase (HAMP) histidine kinase domains. This research expands our comprehension complexities resistance, highlighting multifactorial nature antibiotic mechanisms.

Language: Английский

Citations

0
Impact of Cultivating Different Ocimum Species on Bioaerosol Bacterial Communities and Functional Genome at an Agricultural Site DOI
Anisha Yadav,

Ranu Yadav,

Puja Khare

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 356, P. 124289 - 124289

Published: May 31, 2024

Language: Английский

Citations

2
Carbapenem-Resistant Enterobacter cloacae Complex in Southwest China: Molecular Characteristics and Risk Factors Caused by NDM Producers DOI Creative Commons
Zhangrui Zeng, Yueshuai Wei, Caihong Ye

et al.

Infection and Drug Resistance, Journal Year: 2024, Volume and Issue: Volume 17, P. 1643 - 1652

Published: April 1, 2024

Purpose: The isolation rate of carbapenem-resistant Enterobacter cloacae complex (CREC) is continuously increasing. aims this study were to investigate the molecular characteristics and risk factors associated with CREC infections. Methods: Bacterial species identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Bruker Daltonik GmbH, Bremen, Germany), hsp60 gene was utilized for further typing. Antimicrobial susceptibilities assessed through MicroScan WalkAway 96 Plus system (Siemens, Germany) microbroth dilution method. resistance genes screened polymerase chain reaction (PCR), while homologous relationship multilocus sequence typing (MLST). Conjugation experiments performed verify whether plasmid could be transferred. Additionally, logistic regression model employed analyze Results: 32 strains bacteria isolated during study, yet only 20 retained preservation. While isolates demonstrated majority antibiotics, they exhibited high sensitivity polymyxin B tigecycline. All carried bla NDM gene, including 13 NDM-1 7 NDM-5 isolates. MLST homology analysis revealed presence seven known ST types one new type. confirmed that capable transferring Escherichia coli strain EC600. Single-factor multiple primary infection, but multivariate did not reveal independent factors. Conclusion: This investigates detection in our hospital rising suggested might spread hospital, emphasizing importance implementing effective preventive measures control horizontal transmission plasmid-mediated antimicrobial genes. Keywords: carbapenem-resistant, complex, resistance, characteristics,

Language: Английский

Citations

0
First documentation of a clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring blaNDM-1 and two blaKPC-2 bearing plasmids DOI Creative Commons
Ruyan Chen, Chenyu Li, Hao Xu

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 5, 2024

The increasing prevalence of carbapenem-resistant Enterobacter cloacae complex (CREC) poses great challenges to infection treatment in the clinical setting. In this study, we reported emergence carbapenemase a rare species, chuandaensis, belonging (ECC). We elucidated genetic characteristics isolate FAHZZU5885, co-harboring blaNDM−1 and blaKPC−2. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) average nucleotide identity (ANI) analysis were used identify E. chuandaensis. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) Southern blotting clarify number size plasmids FAHZZU5885. Antimicrobial phenotypes identified by antimicrobial susceptibility testing (AST), strain examined with whole-genome sequencing (WGS). conjugation experiment stability assay conducted verify transferability plasmid carrying carbapenemase-encoding genes. chuandaensis FAHZZU5885 was isolated from perianal swab patient admitted ICU. This simultaneously carried two blaKPC−2 resistant most tested antibiotics except for amikacin, tigecycline, colistin. Two located separately on different plasmids, ~ 120 kb IncFIA-IncFII 80 IncR plasmid. Both shared conserved sequence klcA-korC-ISkpn6-blaKPC−2-ISkpn27-tnpR-tnpA. blaNDM−1-bearing had potential transfer can remain stable after successive passages. addition, IncFII ISAba125-blaNDM−1-ble-trpF-dsbD-cutA-groS-groL. summary, study marks first report multidrug-resistant harboring blaKPC−2-bearing indicating further dissemination genes novel species. findings contribute enhancing our understanding CREC strains, emphasizing need continued comprehensive surveillance

Language: Английский

Citations

0
Surveillance and Characterization of Carbapenem-resistant Enterobacter cloacae Complex from China, 2015-2018 DOI Creative Commons
Kun Ye, Yongqing Zhang, Xuemei Qiu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Abstract Background: The carbapenem-resistant Enterobacter cloacae (CREC) has become a global health concern. However, our understanding of its epidemiological trends and resistance mechanisms still needs to be improved. Methods: 92 non-repetitive clinical CREC isolates were collected from 12 hospitals across China identified using MALDI-TOF MS, rMLST, ANI, MASH, dDDH. Minimal inhibitory concentrations determined via broth microdilution. Resistance genes plasmid replicons ResFinder PlasmidFinder. Carbonyl cyanide 3-chlorophenylhydrazone suppression test OmpC OmpF overexpression conducted on 32 non-carbapenemase-producing (NCP) isolates. A phylogenetic tree was constructed with kSNP4 visualized through iTOL. Results: Among the isolates, E. hormaechei predominate (76 isolates), followed by kobei (five isolates). Within hormaechei, subsp xiangfangensis most widespread subspecies. Sixty produced carbapenemase, blaNDM-1 in 36 blaKPC-2 six isolates, and blaNDM-5 Two harbored both blaKPC-2. Thirty-two NCP primarily due carbonyl suppression. Conclusions: China, dominant carbapenemase-coding gene. For efflux pumps main reason for carbapenem resistance, susceptibility could restored an antibiotic-free environment.

Language: Английский

Citations

0